The CD40L- (or CD154-) knockout mutation is located on the X chromosome. Homozygous females and hemizygous males exhibit humoral immune defects and are a model of human X-linked hyper IgM syndrome.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
---|---|
000664 C57BL/6J |
N?+5N2F9
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Cd40lg | CD40 ligand |
The CD40L- (or CD154-) knockout mutation is located on the X chromosome. Homozygous females and hemizygous males are viable and fertile. Percentages of B and T cell subpopulations are normal. Homozygous females and hemizygous males exhibit selective deficiencies in humoral immunity (low basal serum isotype levels and undetectable IgE) as well as abnormal secondary antigen-specific responses to immunization with a thymus-dependent antigen. The phenotype of the mice resembles human X-linked hyper IgM syndrome.
The CD40L- (or CD154-) knockout mutation was designed by Jacques Peschon (Immunex) to replace exons 3 and 4 of the CD40 ligand gene (Cd40lg) on the X chromosome with a PGKneo cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to C57BL/6 female mice. Heterozygous (129/SV x C57BL/6)F1 females were crossed to C57BL/6 males to generate hemizygous males. CD40L- mice were subsequently backcrossed on to the C57BL/6J background for five generations and then donated to The Jackson Laboratory Repository in 1995 as Stock No. 002770. The mice are maintained on a C57BL/6J genetic background.
Allele Name | targeted mutation 1, Immunex |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD154-; CD40L-; CD40LKO; Tnfsf5tm1Imx |
Gene Symbol and Name | Cd40lg, CD40 ligand |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | X |
Molecular Note | Exons 3 and 4 were replaced with a neomycin cassette. Homozygous mutant animals fail to express functional protein on the cell surface. |
Mutations Made By | Dr. Jacques Peschon, Amgen |
The CD40L- (or CD154-) knockout mutation is located on the X chromosome. Homozygous females and hemizygous males are viable and fertile. When maintaining a live colony, homozygous females may be bred to hemizygous males. Sex ratios among offspring are normal. Expected coat colors from breeding is black.
Homozygous females and hemizygous males are somewhat immunodeficient. As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If CD40L-deficient homozygous mice are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) may be needed to increase overall colony health.
When using the CD40 ligand KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002770 in your Materials and Methods section.
Service/Product | Description | Price |
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X linked - Heterozygous Females and Wild-type Males for Cd40lg<tm1Imx> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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