Mice homozygous for the Mostm1Ev targeted mutation are viable. Homozygous males are fertile; the littersize of homozygous females is markedly lower than that of wild type or heterozygous mice. Eggs lacking Mos undergo spontaneous parthenogenetic activation (extrusion of the second polar body and pronucleus formation without fertilization). Ovarian cysts develop in homozygous females as young as one month. Some of the ovarian cysts consist of several tissue types, including possible thyroid tissue, similar to about 10% of all benign cystic teratomas in human beings.
|Allele Name||targeted mutation 1, Martin L Evans|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Mos, Moloney sarcoma oncogene|
|Strain of Origin||129S/SvEv-Gpi1c|
|Molecular Note||A neomycin resistance cassette was inserted into the middle of the single coding exon of the gene, introducing an amber stop codon into the reading frame and terminating translation upstream of sequences essential for kinase activity.|
|Mutations Made By|| |
Dr. M.J. Evans, University of Cambridge
Homozygous males are fertile and viable while homozygous females have reduced fertility and other reproductive abnormalities (see strain phenotype description). When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. The expected coat color from breeding is black.
When using the mos- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002723 in your Materials and Methods section.