Mice homozygous for the Mostm1Ev targeted mutation are viable. Homozygous males are fertile; the littersize of homozygous females is markedly lower than that of wild type or heterozygous mice. Eggs lacking Mos undergo spontaneous parthenogenetic activation (extrusion of the second polar body and pronucleus formation without fertilization). Ovarian cysts develop in homozygous females as young as one month. Some of the ovarian cysts consist of several tissue types, including possible thyroid tissue, similar to about 10% of all benign cystic teratomas in human beings.
The Mos-deficient strain was developed in the laboratory of Dr. Martin J. Evans at the University of Cambridge, UK. The targeting vector (containing the neo gene) was inserted into the middle of the single exon and introduced an amber stop codon, terminating translation of sequences essential for kinase activity. The construct was electroporated into 129S6/SvEv-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric males were bred to generate heterozygotes. Heterozygotes were crossed to generate homozygotes.
|Allele Name||targeted mutation 1, Martin L Evans|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Mos, Moloney sarcoma oncogene|
|Strain of Origin||129S/SvEv-Gpi1c|
|Molecular Note||A neomycin resistance cassette was inserted into the middle of the single coding exon of the gene, introducing an amber stop codon into the reading frame and terminating translation upstream of sequences essential for kinase activity.|
|Mutations Made By|| |
Dr. M.J. Evans, University of Cambridge
Homozygous males are fertile while homozygous females have reduced fertility. When maintaining a live colony, this strain is maintained by mating heterozygous siblings. Alternatively, heterozygous females can be bred with homozygous males.
When using the mos- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002722 in your Materials and Methods section.