129S6/SvEv-Mos^tm1Ev/J

Stock number: 002722
Product name: mos-
Research areas: Cancer Research, Reproductive Biology Research

Description

Female Mos knock-out mice exhibit reduced fertility.

Detailed description

Mice homozygous for the Mos^tm1Ev targeted mutation are viable. Homozygous males are fertile; the littersize of homozygous females is markedly lower than that of wild type or heterozygous mice. Eggs lacking Mos undergo spontaneous parthenogenetic activation (extrusion of the second polar body and pronucleus formation without fertilization). Ovarian cysts develop in homozygous females as young as one month. Some of the ovarian cysts consist of several tissue types, including possible thyroid tissue, similar to about 10% of all benign cystic teratomas in human beings.

Development

The Mos-deficient strain was developed in the laboratory of Dr. Martin J. Evans at the University of Cambridge, UK. The targeting vector (containing the neo gene) was inserted into the middle of the single exon and introduced an amber stop codon, terminating translation of sequences essential for kinase activity. The construct was electroporated into 129S6/SvEv-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric males were bred to generate heterozygotes. Heterozygotes were crossed to generate homozygotes.

Allele

Symbol: Mos^tm1Ev
Name: targeted mutation 1, Martin L Evans
MGI accession ID: MGI:1857218
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Mos
Marker name: Moloney sarcoma oncogene
Chromosome: 4
Strain of origin: 129S/SvEv-Gpi1^c
Molecular note: A neomycin resistance cassette was inserted into the middle of the single coding exon of the gene, introducing an amber stop codon into the reading frame and terminating translation upstream of sequences essential for kinase activity.