These Wt1 deficient mice are useful for studying cellular development and cell survival.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Wt1 | Wilms tumor 1 homolog |
Mice homozygous for the Wt1tm1Jae targeted mutation die between embryonic days 13 and 15. They fail to develop a kidney or gonads. The hearts of homozygous mutant mice also fail to develop properly. Hearts are smaller than wildtype controls and possess a rounded apex. The right ventricular wall is thin and the left ventricle is reduced in size. There appears to be normal development of the aortic, pulmonary, mitral and tricuspid valves. Development of the diaphragm is also incomplete resulting in incomplete separation of the thoracic and abdominal cavities. Homozygous mutant lungs are also markedly smaller than wild type lungs. Heterozygous mice appear normal and show no tumor development (mice followed until 10 months of age).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
This targeted mutant was made in the Laboratory of Dr. Rudolf Jaenisch at the Whitehead Institute for Biomedical Research. A replacement-type targeting vector was used which resulted in the deletion of the first exon and 0.5 kb of upstream sequence. Targeting was done in J1 ES cells. This strain was produced by backcrossing the Wt1tm1Jae mutation to C57BL/6J. Currently N6 (April, 1997)
Allele Name | targeted mutation 1, Rudolf Jaenisch |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | wt1- |
Gene Symbol and Name | Wt1, Wilms tumor 1 homolog |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 2 |
Molecular Note | Exon 1 and 0.5kb of upstream sequence were replaced with a neomycin selection cassette. |
Mutations Made By | Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology |
The backgound the strain used in the publication was mixed C57BL/6 x 129. The C57BL/6J-Wt1tm1Jae strain is maintained by mating heterozygous carriers to C57BL/6J mice. Expected coat color from breeding:Black
When using the B6.129S4-Wt1tm1Jae/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002719 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Wt1<tm1Jae> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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