Mice homozygous for the Oxttm1Wsy targeted mutation are viable and fertile. Females are able to successfully mate, deliver and produce milk however milk injection is impaired. Pups do not successfully suckle and must be fostered to survive. Oxytocin injection restores milk injection in response to suckling. Homozygotes displayed reduced aggressive behavior relative to heterozygotes and wild-type mice.
This strain was developed in the laboratory of Dr. W. Scott Young at the National Institute of Mental Health. Exons 2 and 3 of the oxytocin gene were replaced with a vector containing the neomycin resistance gene.
|Allele Name||targeted mutation 1, W Scott Young III|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||OT-; OTKO -|
|Gene Symbol and Name||Oxt, oxytocin|
|Strain of Origin||129S/SvEv-Gpi1c|
|Molecular Note||The gene was disrupted using neomycin resistance cassette. The vector replaced sequences encoding the last two exons. Hybridization histochemistry and immunocytochemistry were used to demonstrate the lack of transcript and protein in homozygous mutant animals.|
|Mutations Made By|| |
Dr. W. Scott Young III, National Institute of Mental Health
This strain is maintained by heterozygous sibling matings. Expected coat color from breeding:Black,White Bellied Agouti
When using the OT- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002713 in your Materials and Methods section.