Compared to wild-type mice, these Gabra6 knock-out mice exhibit an absence of a dramatic alteration in behavioral response to neuroactive compounds.
Gregg E Homanics, University of Pittsburgh
Mice homozygous for the Gabra6tm1Geh mutation are viable and fertile with no gross abnormalities. There were no differences detected in behavioral response to ethanol comparing homozygotes to wildtypes from the two background strains, C57BL/6 and 129/SvJ. These findings suggest that the alpha6 subunit of the gamma-aminobutyric acid type A receptor exerts little if any influence on acute functional tolerance, withdrawal hyperexcitability, or protracted tolerance. The only defects discovered thus far are pharmacologic.
Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).
This strain was developed in the laboratory of Dr. Gregg Homanics at the University of Pittsburgh. The vector containing Pgk-neo was inserted by homologous recombination into exon 8 of the Gabra6 gene. The 129X1/SvJ x 129S1/Sv-derived R1 ES cell line was used.
|Allele Name||targeted mutation 1, Gregg E Homanics|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Gabra6, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A neomycin selection cassette was inserted into exon 8. Northern blot analysis on RNA derived from cerebellum of homozygous mice demonstrated that aberrant transcripts were expressed from this allele. Hoever, none of these are predicted to be able to code for a functional protein (J:40242). Later studies showed that this mutation also affected the expression of the Gabra1 and Gabrb2 genes (J:63792).|
|Mutations Made By|| |
Gregg Homanics, University of Pittsburgh
This strain can be maintained on a mixed C57BL/6J x 129 genetic background through homozygous sibling matings. The expected coat colors are black and white-bellied agouti.
When using the B6;129-Gabra6tm1Geh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002710 in your Materials and Methods section.