FVB/N-Tg(Trp53R172L)4491Jmr/J

Stock number: 002660
Product name: 172^Arg→Leu
Research areas: Cancer Research

Description

These 172^Arg→Leu mice express a transgene containing the mouse Trp53 minigene that carries a transversion in codon 172 (CGC→CTC; "Arg172Leu") with altered lobuloalveolar development and increased apoptosis during late pregnancy.

Detailed description

The FVB/N-TgN(Trp53R172L)4491Jmr express TRP53 with pseudo-wildtype properties capable of inducing p21 and mdm-2 expression. Lobuloalveolar development is altered and apoptosis is increased during late pregnancy. The few normal lobules observed during early lactation did not express the Trp53 transgene suggesting that they arose by clonal expansion of cells not expressing the transgene during mid-pregnancy. Transgenic mice fail to lactate. There is no apparent alteration in ductal development. Mice expressing a dominant negative 172Arg-His mutation do not exhibit any detectable alterations in mammary gland development and have a very low incidence of spontaneous mammary tumors. Mice bearing the 172Arg-Leu transgene and a pituitary isograft displayed a marked increase in apoptosis and a significant delay in DMBA-induced tumorigenesis, while those bearing the 172Arg-His dominant negative p53 transgene were more susceptible to DMBA-induced tumorigenesis.

Development

0.95 kb of the 5' flanking sequence of the rat WAP (-949/+33) gene fused to a murine p53 minigene as described in Lozano, G. and Levine, A.J. Mol. Carcinog.4:3-9, 1991. Removal of the ts mutation in the original p53 minigene at codon 135(Ala-Val) and introduction of either an Arg-Leu or an Arg-His mutation at aa 172 was accomplished using recombinant PCR mutagenesis. Transgenic mice were generated by microinjection into FVB mouse embryos. (Biol. Mammary Gland, WWW)

Allele

Symbol: Tg(Trp53R172L)4491Jmr
Name: transgene insertion 4491, Jeffrey Rosen
MGI accession ID: MGI:1929643
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Trp53R172L)4491Jmr
Marker name: transgene insertion 4491, Jeffrey Rosen
Chromosome: UN
Strain of origin: FVB
Expressed genes: Trp53,transformation related protein 53,mouse, laboratory
Molecular note: This transgene contains the rat whey acidic protein promoter (0.95 kb of 5' sequence) fused to a mouse Trp53 minigene that carries a transversion in codon 172 (CGC->CTC; "Arg172Leu"). Removal of the ts mutation in the original Trp53 minigene at codon 135 (Ala-Val) and introduction of the Arg-Leu mutation at amino acid 172 were accomplished using recombinant PCR mutagenesis.