These 172Arg→Leu mice express a transgene containing the mouse Trp53 minigene that carries a transversion in codon 172 (CGC→CTC; "Arg172Leu") with altered lobuloalveolar development and increased apoptosis during late pregnancy.
Dr. Jeffrey Rosen, Baylor College of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
The FVB/N-TgN(Trp53R172L)4491Jmr express TRP53 with pseudo-wildtype properties capable of inducing p21 and mdm-2 expression. Lobuloalveolar development is altered and apoptosis is increased during late pregnancy. The few normal lobules observed during early lactation did not express the Trp53 transgene suggesting that they arose by clonal expansion of cells not expressing the transgene during mid-pregnancy. Transgenic mice fail to lactate. There is no apparent alteration in ductal development. Mice expressing a dominant negative 172Arg-His mutation do not exhibit any detectable alterations in mammary gland development and have a very low incidence of spontaneous mammary tumors. Mice bearing the 172Arg-Leu transgene and a pituitary isograft displayed a marked increase in apoptosis and a significant delay in DMBA-induced tumorigenesis, while those bearing the 172Arg-His dominant negative p53 transgene were more susceptible to DMBA-induced tumorigenesis.
0.95 kb of the 5' flanking sequence of the rat WAP (-949/+33) gene fused to a murine p53 minigene as described in Lozano, G. and Levine, A.J. Mol. Carcinog.4:3-9, 1991. Removal of the ts mutation in the original p53 minigene at codon 135(Ala-Val) and introduction of either an Arg-Leu or an Arg-His mutation at aa 172 was accomplished using recombinant PCR mutagenesis. Transgenic mice were generated by microinjection into FVB mouse embryos. (Biol. Mammary Gland, WWW)
Expressed Gene | Trp53, transformation related protein 53, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 4491, Jeffrey Rosen |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Trp53R172L)4491Jmr, transgene insertion 4491, Jeffrey Rosen |
Gene Synonym(s) | |
Promoter | Wap, whey acidic protein, rat |
Expressed Gene | Trp53, transformation related protein 53, mouse, laboratory |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | This transgene contains the rat whey acidic protein promoter (0.95 kb of 5' sequence) fused to a mouse Trp53 minigene that carries a transversion in codon 172 (CGC->CTC; "Arg172Leu"). Removal of the ts mutation in the original Trp53 minigene at codon 135 (Ala-Val) and introduction of the Arg-Leu mutation at amino acid 172 were accomplished using recombinant PCR mutagenesis. |
Mutations Made By | Dr. Jeffrey Rosen, Baylor College of Medicine |
This strain can be maintained through FVB x hemizygous crosses. The expected coat color from breeding is albino.
When using the 172Arg→Leu mouse strain in a publication, please cite the originating article(s) and include JAX stock #002660 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Trp53R172L)4491Jmr |
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