These En1 knock-out mice exhibit neonatal mortality with multiple developmental defects of the brain and other tissues.
Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center
Mice homozygous for the En1tm1Alj targeted mutation die shortly after birth. They are missing the third and fourth cranial nerves as well as most of the colliculi and cerebellum. The brain phenotype can be less severe depending on the genetic background. There is also a disruption of the dorsal/ventral patterning of the limb paws, a disrupted sterum, and truncation of the 13th ribs. Deletion of mid-hindbrain tissue may be seen as early as embryonic day 9.5.
|Allele Name||targeted mutation 1, Alexandra L Joyner|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||En1, engrailed 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A neomycin selection cassette replaced 0.85 kb of exon 2, which encodes part of the homeobox domain.|
|Mutations Made By|| |
Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center
Homozygotes die shortly after birth. When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. The expected coat color from breeding is black.
When using the En1hd mouse strain in a publication, please cite the originating article(s) and include JAX stock #002656 in your Materials and Methods section.