STOCK En1^tm1Alj/J

Stock number: 002656
Product name: En1^hd
Research areas: Developmental Biology Research, Neurobiology Research

Description

These En1 knock-out mice exhibit neonatal mortality with multiple developmental defects of the brain and other tissues.

Detailed description

Mice homozygous for the En1^tm1Alj targeted mutation die shortly after birth. They are missing the third and fourth cranial nerves as well as most of the colliculi and cerebellum. The brain phenotype can be less severe depending on the genetic background. There is also a disruption of the dorsal/ventral patterning of the limb paws, a disrupted sterum, and truncation of the 13th ribs. Deletion of mid-hindbrain tissue may be seen as early as embryonic day 9.5.

Allele

Symbol: En1^tm1Alj
Name: targeted mutation 1, Alexandra L Joyner
MGI accession ID: MGI:1857163
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: En1
Marker name: engrailed 1
Chromosome: 1
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A neomycin selection cassette replaced 0.85 kb of exon 2, which encodes part of the homeobox domain.