B6;129S4-H2-DMa^tm1Luc/J

Stock number: 002643
Product name: H2-Mα-
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These H2-DMa knock-out mice exhibit defects in immunological responses.

Detailed description

Mice homozygous for the H2-DMa^tm1Luc targeted mutation are viable and fertile but generally smaller than wildtype siblings. Defects in immunological responses in homozygous mutant mice include defects in loading of peptides into the MHC class II molecules, presentation of antigen in a MHC class II restricted manner, and intrathymic T-cell positive and negative selection. This strain may be useful as a model for examination of MHC class II restricted antigen presentation, tissue graft rejection, and T-cell repertoire selection.

Development

This strain was developed in the laboratory of Dr. Luc Van Kaer at Vanderbilt University School of Medicine. The 129-derived J1 ES cell line was used. A 1.5kb piece of the H2-DMa gene from all of exon 1 and most of exon 2 was replaced with a vector containing the neomycin resistance gene.

Allele

Symbol: H2-DMa^tm1Luc
Name: targeted mutation 1, Luc van Kaer
MGI accession ID: MGI:1861933
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: H2-DMa
Marker name: histocompatibility 2, class II, locus DMa
Chromosome: 17
Strain of origin: 129S4/SvJae
Molecular note: A neomycin resistance cassette replaced a 1.5 kb fragment of the gene, encompassing the first and most of the second exon.