These H2-DMa knock-out mice exhibit defects in immunological responses.
Luc van Kaer, Vanderbilt University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | H2-DMa | histocompatibility 2, class II, locus DMa |
Mice homozygous for the H2-DMatm1Luc targeted mutation are viable and fertile but generally smaller than wildtype siblings. Defects in immunological responses in homozygous mutant mice include defects in loading of peptides into the MHC class II molecules, presentation of antigen in a MHC class II restricted manner, and intrathymic T-cell positive and negative selection. This strain may be useful as a model for examination of MHC class II restricted antigen presentation, tissue graft rejection, and T-cell repertoire selection.
This strain was developed in the laboratory of Dr. Luc Van Kaer at Vanderbilt University School of Medicine. The 129-derived J1 ES cell line was used. A 1.5kb piece of the H2-DMa gene from all of exon 1 and most of exon 2 was replaced with a vector containing the neomycin resistance gene.
Allele Name | targeted mutation 1, Luc van Kaer |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | H2-DM-; H-2M-; H2-M-; H2-M0; H2-Malpha- |
Gene Symbol and Name | H2-DMa, histocompatibility 2, class II, locus DMa |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 17 |
Molecular Note | A neomycin resistance cassette replaced a 1.5 kb fragment of the gene, encompassing the first and most of the second exon. |
Mutations Made By | Dr. Luc Van Kaer, Vanderbilt University School of Medicine |
Mice homozygous for the H2-Matm1Luc targeted mutation are viable and fertile but generally smaller than wildtype siblings. When maintaining a live colony, homozygous mice may be bred together. The expected coat color from breeding is black.
When using the H2-Mα- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002643 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for DMa<tm1Luc> |
Frozen Mouse Embryo | B6;129S4-H2-DMa<tm1Luc>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S4-H2-DMa<tm1Luc>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S4-H2-DMa<tm1Luc>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S4-H2-DMa<tm1Luc>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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