Mice homozygous for the Gfaptm1Me targeted mutation are viable and fertile. Homozygous mutant mice show subtle changes in astrocyte morphology, CNS physiology, and enhanced LTP of both population spike amplitude and EPSP slope compared to control mice. Homozygotes are also hypersensitive to cervical spinal cord injury. These mice may be useful in studies dealing with transplantation, injury, or astrocyte biology.
This strain was developed in the laboratory of Dr. Albee Messing at the University of Wisconsin School of Veterinary Medicine. This stain is on a mixed C57BL/6 and 129 genetic background.
|Allele Name||targeted mutation 1, Albee Messing|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Gfap, glial fibrillary acidic protein|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|Molecular Note||A neomycin cassette replaced most of exon 1 and all of exons 2-4. Northern blot analysis revealed that there was no mRNA produced from this allele in whole brain RNA samples derived from homozygous mice. Immunohistochemical studies detected no GFAP protein present in sections of hippocampus in homozygous mice.|
|Mutations Made By|| |
Dr. Albee Messing, University of Wisconsin-Madison
This strain may be maintained by mating homozygous siblings. The expected coat color is black.
When using the GFAP KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002642 in your Materials and Methods section.