These mice carry the spontaneous mutation patchwork (pwk) characterized by hairs that are either totally white or totally pigmented due to the presence of or absence of melanotyes in the hair follicles.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | pwk | patchwork |
Homozygous patchwork mice have hairs that are either totally white or totally pigmented, but no diluted coloration of the hair. On a nonagouti background this produces a salt-and-pepper appearance from white hairs juxtaposed with black hairs. This occurs throughout the coat and does not vary by anatomic region. The absence of pigment in the white hairs is due to an absence of melanocytes in the hair follicles of the white hairs; functioning melanocytes are present in the hair follicles of the black hairs. The absence of melanotyes in the follicles of the white hairs results from premature death of melanoblasts during development. TUNEL staining indicates that this melanoblast death is apoptotic and begins around embryonic day 18.5. This suggests that patchwork melanoblasts can survive and function if enough survive, but fail to survive when adequately reduced in number. Analysis of aggregation chimeras between patchwork and albino donors revealed gray hairs at the boundaries between the white and black hairs indicating that the albino melanoblasts could enhance the survival of patchwork melanoblasts. Thus, the patchwork defect is not strictly melanoblast autonomous. (Aubin-Houzelstein et al., 1998.)
pwk is a spontaneous mutation that was identified in an outbred stock referred to as Pm stock. Pm stock was homozygous for at and segregating for Tyrp1b, Tyrc, Ednrbs, Foxq1sa, p, Myo5ad, and Fgf5go. pwk was backcrossed 4 times via backcross-intercross from this Pm stock onto C57BL/6. Then, in 1993 Dr. Karen Moore sent N4F1 males homozygous for pwk to Dr. Eva Eicher at The Jackson Laboratory. pwk was then backcrossed onto the C57BL/6JEi background, and in 1996 C57BL/6J females were bred with N9F2 B6Ei.Cg-pwk homozygotes to generate embryos for cryopreservation.
When using the patchwork mouse strain in a publication, please cite the originating article(s) and include JAX stock #002622 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for pwk |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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