This strain is deficient of tumor necrosis factor receptor superfamily, member 1b (Tnfrsf1b), and are consequently resistant to TNF-induced cell death.
Dr. Mark W. Moore, Deltagen
Genetic Background | Generation |
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N10F59
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tnfrsf1b | tumor necrosis factor receptor superfamily, member 1b |
Mice homozygous for a Tnfrsf1btm1Mwm targeted mutation (formerly Tnfr2tm1Mwm, p75 deficient) are viable and fertile. Homozygous mutant mice show normal T-cell development and activity, but are resistant to TNF-induced cell death. Subcutaneous injections of TNF into homozygotes elicit much less tissue necrosis.
This strain was developed by using homologous recombination techniques to target the Tnfr2 gene. The inserted construct contained a neomycin resistance gene under the control of the Pgk promoter and was inserted into the second exon of the Tnfr2 gene.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 1, Mark Moore |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | p75-; p75TNFR2-; TNFR II -; TNF-R2-; TNFR20; Tnfr2tm1Mwm; TNFRp75- |
Gene Symbol and Name | Tnfrsf1b, tumor necrosis factor receptor superfamily, member 1b |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 4 |
Molecular Note | Exon 2, which codes for the signal peptide region, was disrupted by the insertion of a neomycin cassette. To confirm that the gene was correctly targeted, mice were challenged with lipopolysaccharide (LPS) which results in shedding of the Tnfrsf1b extracellular domian in wild-type mice. No soluble Tnfrsf1b was detected in the serum of homozygous mutant animals. |
Mutations Made By | Dr. Mark Moore, Deltagen |
This strain was generated on a mixed 129 and C57BL/6J background. It has been backcrossed to C57BL/6 six times. Homozygotes are viable and fertile. The strain is maintained by mating homozygous siblings. Expected coat color from breeding:Black
When using the TNF-R2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002620 in your Materials and Methods section.
Service/Product | Description | Price |
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Homozygous for Tnfrsf1b<tm1Mwm>, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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