This strain is deficient of tumor necrosis factor receptor superfamily, member 1b (Tnfrsf1b), and are consequently resistant to TNF-induced cell death.
Dr. Mark W. Moore, Deltagen
Mice homozygous for a Tnfrsf1btm1Mwm targeted mutation (formerly Tnfr2tm1Mwm, p75 deficient) are viable and fertile. Homozygous mutant mice show normal T-cell development and activity, but are resistant to TNF-induced cell death. Subcutaneous injections of TNF into homozygotes elicit much less tissue necrosis.
This strain was developed by using homologous recombination techniques to target the Tnfr2 gene. The inserted construct contained a neomycin resistance gene under the control of the Pgk promoter and was inserted into the second exon of the Tnfr2 gene.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Mark Moore|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||p75-; p75TNFR2-; TNFR II -; TNF-R2-; TNFR20; Tnfr2tm1Mwm; TNFRp75-|
|Gene Symbol and Name||Tnfrsf1b, tumor necrosis factor receptor superfamily, member 1b|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Exon 2, which codes for the signal peptide region, was disrupted by the insertion of a neomycin cassette. To confirm that the gene was correctly targeted, mice were challenged with lipopolysaccharide (LPS) which results in shedding of the Tnfrsf1b extracellular domian in wild-type mice. No soluble Tnfrsf1b was detected in the serum of homozygous mutant animals.|
|Mutations Made By|| |
Dr. Mark Moore, Deltagen
This strain was generated on a mixed 129 and C57BL/6J background. It has been backcrossed to C57BL/6 six times. Homozygotes are viable and fertile. The strain is maintained by mating homozygous siblings. Expected coat color from breeding:Black
When using the TNF-R2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002620 in your Materials and Methods section.