| Allele Name | transgene insertion 1, Lothar Hennighausen |
| Allele Type | Transgenic (Transactivator) |
| Allele Synonym(s) | MMTV-tTA |
| Gene Symbol and Name | Tg(MMTVtTA)1Mam, transgene insertion 1, Lothar Hennighausen |
| Gene Synonym(s) | |
| Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV |
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| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
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| Site of Expression | Expresses tTA in the epithelial cells of secretory organs and skin. When mated to a tetop-lacz reporter mouse, nearly uniform expression of lacZ was found in seminal vesicle, salivary gland, and Leydig cells. More heterogeneous patterns of lacZ expression were observed in mammary epithelial cells of the epidermis. |
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| Strain of Origin | C57BL/6 and SJL |
| Chromosome | UN |
| General Note | Additional lines were generated and showed comparable tissue expression patterns. When transgenic mice were mated to mice transgenic for either lacZ or luciferase reporter genes coupled to tetracycline-responsive promoter elements (TRE), nearly uniform expression of the reporter was found in seminal vesicle, salivary gland, and Leydig cells of the double transgenic offspring. More heterogeneous patterns of reporter expression were observed in mammary epithelial cells and basal cells of the epidermis. Lower levels of reporter gene expression were also observed in a broad range of tissues. Transcriptional activation mediated by tTA was up to several hundredfold and was abrogated after the administration of tetracycline. In combination with Tg(BCR/ABL1)2Dgt, when tetracycline administration is stopped, bitransgenic mice are models for B cell acute lymphoblastic leukemia (ALL). (J:72377) |
| Molecular Note | The MMTV LTR was used to target expression of tetracycline-controlled transactivator protein (tTA) to the epithelial cells of secretory organs and skin. The tetracycline resistance gene (TetR) was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). |
| Mutations Made By | Dr. Lothar Hennighausen, National Institutes of Health |
