Mice homozygous for the Bmp4tm1Blh targeted mutation mice die early in embryogenesis. Fifteen to twenty percent of heterozygous mice show developmental defects including polydactyly, craniofacial defects, and cystic kidneys. Heterozygotes have also been reported to show eye abnormalities.
Brigid LM Hogan, Duke University Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Bmp4 | bone morphogenetic protein 4 |
Mice homozygous for the Bmp4tm1Blh targeted mutation mice die early in embryogenesis. Fifteen to twenty percent of heterozygous mice show developmental defects including polydactyly, craniofacial defects, and cystic kidneys. Heterozygotes have also been reported to show eye abnormalities.
This strain was developed in the laboratory of Dr. Brigid Hogan at Vanderbilt Medical School. The 129S2/SvPas-derived D3 ES cell line was used. The mutation was backcrossed to the C57BL/6 background for 11 generations (N11).
Allele Name | targeted mutation 1, Brigid L Hogan |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Bmp4-; Bmp4delta; Bmp4tm1; Bmp-4tm1blh |
Gene Symbol and Name | Bmp4, bone morphogenetic protein 4 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 14 |
Molecular Note | A neomycin selection cassette replaced a genomic fragment containing all of the coding sequence of the gene after the seventh codon of exon 1. An oligonucleotide encoding a stop codon in all three reading frames was also inserted into exon 2. |
Mutations Made By | Brigid Hogan, Duke University Medical Center |
The strain is maintained by breeding heterozygous mice to normal wildtype siblings or C57BL/6 inbred mice. Heterozygous breeder pairs are supplied. Female heterozygous mice appear to be poorer breeders than wildtype females and often have an inner ear defect that may cause the mice to spin. Heterozygous breeders are selected for the least severe expression of the inner ear defect. Expected coat color from breeding:Black
When using the B6.129S2-Bmp4tm1Blh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002612 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Bmp4<tm1Blh> |
Frozen Mouse Embryo | B6.129S2-Bmp4<tm1Blh>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Bmp4<tm1Blh>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Bmp4<tm1Blh>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S2-Bmp4<tm1Blh>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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