B6.129S2-Bmp4^tm1Blh/J

Stock number: 002612
Research areas: Cancer Research, Dermatology Research, Developmental Biology Research, Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Reproductive Biology Research, Sensorineural Research

Detailed description

Mice homozygous for the Bmp4^tm1Blh targeted mutation mice die early in embryogenesis. Fifteen to twenty percent of heterozygous mice show developmental defects including polydactyly, craniofacial defects, and cystic kidneys. Heterozygotes have also been reported to show eye abnormalities.

Development

This strain was developed in the laboratory of Dr. Brigid Hogan at Vanderbilt Medical School. The 129S2/SvPas-derived D3 ES cell line was used. The mutation was backcrossed to the C57BL/6 background for 11 generations (N11).

Allele

Symbol: Bmp4^tm1Blh
Name: targeted mutation 1, Brigid L Hogan
MGI accession ID: MGI:1857137
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Bmp4
Marker name: bone morphogenetic protein 4
Chromosome: 14
Strain of origin: 129S2/SvPas
Molecular note: A neomycin selection cassette replaced a genomic fragment containing all of the coding sequence of the gene after the seventh codon of exon 1. An oligonucleotide encoding a stop codon in all three reading frames was also inserted into exon 2.