Overview

Mice homozygous for the Bmp4^tm1Blh targeted mutation mice die early in embryogenesis. Fifteen to twenty percent of heterozygous mice show developmental defects including polydactyly, craniofacial defects, and cystic kidneys. Heterozygotes have also been reported to show eye abnormalities.

Donating Investigator

Brigid LM Hogan, Duke University Medical Center

Genetic overview

Genetic Background	Generation

Bmp4^tm1Blh

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Bmp4	bone morphogenetic protein 4

View Genetics

Research Applications

Internal/Organ Research
Sensorineural Research
Developmental Biology Research
Reproductive Biology Research
Endocrine Deficiency Research
Immunology, Inflammation and Autoimmunity Research
Cancer Research
Dermatology Research

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Details

Detailed Description

Mice homozygous for the Bmp4^tm1Blh targeted mutation mice die early in embryogenesis. Fifteen to twenty percent of heterozygous mice show developmental defects including polydactyly, craniofacial defects, and cystic kidneys. Heterozygotes have also been reported to show eye abnormalities.

Development

This strain was developed in the laboratory of Dr. Brigid Hogan at Vanderbilt Medical School. The 129S2/SvPas-derived D3 ES cell line was used. The mutation was backcrossed to the C57BL/6 background for 11 generations (N11).

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Winnier G; Blessing M; Labosky PA; Hogan BL. 1995. Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse. Genes Dev 9(17):2105-16PubMed: 7657163 MGI: J:28717

View All References

Genetics

Bmp4^tm1Blh

Allele Symbol: Bmp4^tm1Blh

Allele Name	targeted mutation 1, Brigid L Hogan
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Bmp4^-; Bmp4^delta; Bmp4^tm1; Bmp-4^tm1blh
Gene Symbol and Name	Bmp4, bone morphogenetic protein 4
Gene Synonym(s)
Strain of Origin	129S2/SvPas
Chromosome	14
Molecular Note	A neomycin selection cassette replaced a genomic fragment containing all of the coding sequence of the gene after the seventh codon of exon 1. An oligonucleotide encoding a stop codon in all three reading frames was also inserted into exon 2.
Mutations Made By	Brigid Hogan, Duke University Medical Center

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

Axenfeld-Rieger syndrome type 3

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Bmp4^tm1Blh related

Internal/Organ Research
- Kidney Defects
Sensorineural Research
- Eye Defects
Developmental Biology Research
- Internal/Organ Defects
  - kidney
- Growth Defects
- Skeletal Defects
- Craniofacial and Palate Defects
- Embryonic Lethality (Homozygous)
- Eye Defects
Reproductive Biology Research
- Developmental Defects Affecting Gonads
- Fertility Defects
Endocrine Deficiency Research
- Bone/Bone Marrow Defects
- Gonad Defects
- Kidney Defects
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines
Cancer Research
- Genes Regulating Growth and Proliferation
- Growth Factors/Receptors/Cytokines
Dermatology Research
- Skin and Hair Texture Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss

cardiovascular system phenotype

abnormal heart looping
- at the 9 to 10 somite stage in about 50% of embryos the heart tube lies centrally along the midline and shows no signs of looping

embryo phenotype

abnormal mesoderm development
- a mass of mesodermal cells extending to the apical surface of the ectoderm fills much of the posterior amniotic cavity

abnormal primitive node morphology
- one embryo had large endoderm-like cells within the node
- node is either flat or slightly convex with an irregular periphery in embryos from the headfold to 6 somite stage unlike in controls where it is concave

abnormal lateral plate mesoderm morphology
- at the 2 to 8 somite stage, over 75% of embryos show patch or reduced Nodal expression and lack expression in the left lateral plate mesoderm
- in about 65% of embryos at the 3 to 4 somite stage Lefty2 expression is absent from the lateral plate mesoderm

abnormal amniotic cavity morphology
- much of the posterior amniotic cavity is filled up by a mass of mesodermal cells that extends to the apical surface of the ectoderm

abnormal posterior primitive streak morphology
- an abnormal posterior bulge is present from the headfold to 6 somite stage

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * C57BL/6

embryo phenotype

abnormal extraembryonic mesoderm development
- absent extraembryonic mesoderm

absent visceral yolk sac blood islands
- embryos that develop past the egg cylinder stage have a paucity of blood islands

embryonic growth retardation
- embryos are grossly retarded but have undergone turning, have a beating heart and forelimb buds

abnormal visceral yolk sac mesenchyme morphology
- paucity of extraembryonic mesoderm underlying the endoderm layer

abnormal visceral yolk sac morphology
- visceral yolk sac often have a "blebby" appearance attributable to the paucity of extraembryonic mesoderm and blood islands underlying the endoderm layer

abnormal rostral-caudal axis patterning
- posterior patterning abnormalities

growth/size/body region phenotype

embryonic growth retardation
- embryos are grossly retarded but have undergone turning, have a beating heart and forelimb buds

mortality/aging

embryonic lethality, complete penetrance
- animals die between E7.5 and E10.5; most are arrested at the egg cylinder stage

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * BALB/cJ

vision/eye phenotype

persistence of hyaloid vascular system
- Background Sensitivity - only 1 out of 15 mice on a BALB/cJ background exhibit eye abnormalities (persistent vitreous vessels) compared to multiple and variable eye abnormalities seen on a C57BL/6J background

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * C57BL/6 * CBA

behavior/neurological phenotype

circling
- in 22 of 60 of mice with bilateral lateral semicircular canal defects

cellular phenotype

decreased primordial germ cell number
- fewer PGCs due to a reduced founder population rather than impaired expansion
- fewer Dppa3+ primordial germ cells than in wild-type mice
- estimated 62% reduction in PGC founder population of mice on the C57BL/6, CBA mixed background
- PGCs were absent in 9% of mutant mice on the C57BL/6, CBA mixed background

hearing/vestibular/ear phenotype

abnormal lateral semicircular canal morphology
- Background Sensitivity - only 9% of mice exhibit abnormal semicircular canal on a mixed 129S2/SvPas, C57BL/6, and CBA background compared to 70% of females and 62% of males on a mixed 129S2/SvPas and C57BL/6 background

reproductive system phenotype

decreased primordial germ cell number
- estimated 62% reduction in PGC founder population of mice on the C57BL/6, CBA mixed background
- fewer PGCs due to a reduced founder population rather than impaired expansion
- fewer Dppa3+ primordial germ cells than in wild-type mice
- PGCs were absent in 9% of mutant mice on the C57BL/6, CBA mixed background

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * 129S6/SvEvTac

cellular phenotype

decreased primordial germ cell number
- estimated 55% reduction in PGC founder population of mice on the 129S/SvEv, Black Swiss mixed background
- fewer primordial germ cells due to a reduced founder population rather than impaired expansion

reproductive system phenotype

decreased primordial germ cell number
- estimated 55% reduction in PGC founder population of mice on the 129S/SvEv, Black Swiss mixed background
- fewer primordial germ cells due to a reduced founder population rather than impaired expansion

vision/eye phenotype

abnormal eye morphology
- Background Sensitivity - incidence of eye abnormalities is much lower on the 129S6/SvEvTac background than on a C57BL/6J background, with only 1 of 12 mice showing persistent vitreous vessels and abnormal pupil/iris

abnormal iris morphology
- 1 of 12 mice shows persistent vitreous vessels

persistence of hyaloid vascular system
- 1 of 12 mice shows abnormal pupil/iris

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * Black Swiss

cellular phenotype

absent primordial germ cells

reproductive system phenotype

absent primordial germ cells

vision/eye phenotype

abnormal lens induction
- failure of lens placode induction

embryo phenotype

embryonic growth retardation
- at E10.5, homozygotes have 20-27 somites, while littermates have greater than 35

absent allantois
- all embryos lacked an allantois

endocrine/exocrine gland phenotype

absent Rathke's pouch
- no sign of Rathke's pouch formation (either ectodermal thickening or invagination) is noted at E9.5-E9.75

growth/size/body region phenotype

embryonic growth retardation
- at E10.5, homozygotes have 20-27 somites, while littermates have greater than 35

mortality/aging

embryonic lethality during organogenesis, complete penetrance
- at E10.5

nervous system phenotype

absent Rathke's pouch
- no sign of Rathke's pouch formation (either ectodermal thickening or invagination) is noted at E9.5-E9.75

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * C57BL/6 * CBA

cellular phenotype

absent primordial germ cells
- absent primordial germ cells

embryo phenotype

absent allantois
- all embryos lacked an allantois

embryonic growth retardation

growth/size/body region phenotype

embryonic growth retardation

mortality/aging

prenatal lethality, complete penetrance

reproductive system phenotype

absent primordial germ cells
- absent primordial germ cells

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
129S2/SvPas-Bmp4

embryo phenotype

abnormal extraembryonic mesoderm development
- hypoplastic extraembryonic mesoderm: small amount can be distinguished

abnormal visceral yolk sac morphology

absent visceral yolk sac blood islands

failure of primitive streak formation
- absence of organized primitive streak

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance
- none survive beyond the egg cylinder stage

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * ICR

embryo phenotype

embryonic growth retardation
- are grossly retarded but have undergone turning, have a beating heart and forelimb buds

growth/size/body region phenotype

embryonic growth retardation
- are grossly retarded but have undergone turning, have a beating heart and forelimb buds

mortality/aging

embryonic lethality during organogenesis, complete penetrance
- embryos die between E9.5-E11.5

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * C57BL/6

behavior/neurological phenotype

circling
- in 37% of females and 24% of males strongly associated with bilateral defects in the lateral semicircular canal

renal/urinary system phenotype

abnormal renal glomerulus morphology
- mice show attenuation of mesangial expansion compare to diabetic (STZ-treated) wild-type

growth/size/body region phenotype

decreased body weight
- diabetic mutants (generated by treatment with streptozotocin, STZ) exhibit decreased body weight compared to untreated controls

hearing/vestibular/ear phenotype

abnormal lateral semicircular canal morphology
- Background Sensitivity - 70% of females and 62% of males on a mixed 129S2/SvPas and C57BL/6 background exhibit abnormal semicircular canal compared to only 9% of mice on a mixed 129S2/SvPas, C57BL/6, and CBA background
- higher in the left ear than the right ear

abnormal ear development
- at E12, mice exhibit small or absent lateral plate epithelium compared with wild-type mice with the central part least affected of all the parts

absent lateral semicircular canal
- the lateral duct and cartilage lining are absent in some mice

decreased lateral semicircular canal size
- partially truncated, constricted, or absent in some mice

homeostasis/metabolism phenotype

increased circulating glucose level
- diabetic mutants (generated by treatment with streptozotocin, STZ) have increased blood glucose similar to STZ-treated wild-type mice

mammalian phenotype

hearing/vestibular/ear phenotype
- Normal - mice exhibit normal hearing

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * C3Hf/HeA * C57BL/LiA

vision/eye phenotype

mydriasis
- 5 of 13 mutants exhibit enlarged pupils

abnormal anterior eye segment morphology
- Background Sensitivity - on a C3Hf/HeA and C57BL/LiA background, fewer mutants exhibit anterior segment abnormalities, with only half showing defects compared to 2/3 of mutants on a C57BL/6J background

abnormal iridocorneal angle

buphthalmos

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss

mammalian phenotype

behavior/neurological phenotype
- Background Sensitivity - mice do not display circling behavior on Black Swiss background but a small percentage do on a C57BL/6 background

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * CAST/Ei

vision/eye phenotype

cataract
- Background Sensitivity - on a CAST/Ei background, only one of seven mice develops a cataract compared to multiple and variable eye abnormalities in mice on a C57BL/6J background

Genotype: Bmp4^tm1Blh/Bmp4^tm4Blh
involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss * C57BL/6 * SJL

hearing/vestibular/ear phenotype

abnormal semicircular canal morphology
- percentage of mice display lateral canal truncation

The following phenotype relates to a compound genotype created using this strain

Genotype: Bmp4^tm1Blh/Bmp4⁺
B6.129S2-Bmp4

behavior/neurological phenotype

abnormal vestibulocollic reflex
- circling heterozygotes display low gain ratios with yaw axis rotation in the vestibulo-collic reflex, indicating poorer head stability relative to wild-type mice; poor response is consistent with horizontal semicircular canal dysfunction
- in the pitch axis, circling heterozygotes display as good or better head stability than wild-type mice, with gain ratios being greater or equal to 1
- Normal - in contrast, non-circling heterozygotes have gain ratios of greater or equal to 1 in both the yaw and pitch axes, indicating normal VCR function

circling
- the second circler spent 65% of the time circling in a clockwise direction, 1% in a counterclockwise direction and 34% not circling
- 1 of 2 circlers spent 50% of the time circling in a clockwise direction, 17% in a counterclockwise direction and 33% not circling
- by 2 weeks of age, 10% of heterozygous pups display circling behavior

endocrine/exocrine gland phenotype

abnormal prostate gland anterior lobe morphology
- sometimes cystic
- sometimes with abnormal lobulation

abnormal seminiferous tubule morphology
- sometimes cystic

hearing/vestibular/ear phenotype

decreased vestibular hair cell stereocilia number
- 4 of 6 circling heterozygotes display a significantly reduced number of stereocilia in their ampullae relative to wild-type; the other two circlers show a patchy decrease in the number of stereocilia
- Normal - in contrast, circling heterozygotes show normal stereocilia in the organ of Corti, saccule and utricle
- Normal - non-circling heterozygotes have normal-appearing stereocilia in both auditory and vestibular tissues

impaired hearing
- both circling and non-circling heterozygotes display a partial hearing loss, as assessed by ABR testing

increased or absent threshold for auditory brainstem response
- non-circlers display elevated ABR thresholds similar to those of circlers
- most circling heterozygotes exhibit elevated ABR thresholds across all test frequencies (7 out of 11 mice at 6 kHz and 12 kHz; 8 out of 11 mice at 24 kHz)

abnormal organ of Corti morphology
- Normal - in contrast, the saccule, utricle and ampullae of heterozygotes show normal numbers of neuronal processes
- 2 of 4 circler and 2 of 4 non-circler heterozygotes show reduced neuronal processes in the organ of Corti

abnormal vestibulocollic reflex
- in the pitch axis, circling heterozygotes display as good or better head stability than wild-type mice, with gain ratios being greater or equal to 1
- circling heterozygotes display low gain ratios with yaw axis rotation in the vestibulo-collic reflex, indicating poorer head stability relative to wild-type mice; poor response is consistent with horizontal semicircular canal dysfunction
- Normal - in contrast, non-circling heterozygotes have gain ratios of greater or equal to 1 in both the yaw and pitch axes, indicating normal VCR function

limbs/digits/tail phenotype

polydactyly
- 12% with unilateral anterior polydactyly involving the right hind limb only

mortality/aging

perinatal lethality, incomplete penetrance
- less than half as many heterozygotes are born as expected

nervous system phenotype

absent optic nerve
- the optic nerve is sometimes absent

abnormal optic nerve morphology
- optic nerve abnormalities range from normal to absent, and are most often severely abnormal consisting of loose connective tissue, with absence of neural tissue

decreased vestibular hair cell stereocilia number
- 4 of 6 circling heterozygotes display a significantly reduced number of stereocilia in their ampullae relative to wild-type; the other two circlers show a patchy decrease in the number of stereocilia
- Normal - in contrast, circling heterozygotes show normal stereocilia in the organ of Corti, saccule and utricle
- Normal - non-circling heterozygotes have normal-appearing stereocilia in both auditory and vestibular tissues

abnormal optic disk morphology
- abnormalities of the optic nerve head are frequently observed

abnormal sensory neuron innervation pattern
- neuronal processes innervating the cochlea of both circling and non-circling heterozygotes mice are reduced in number

renal/urinary system phenotype

single kidney
- single cystic kidney in about 12% of heterozygotes

kidney atrophy
- cortex of remaining kidney is atrophic

kidney cysts
- single cystic kidney in about 12% of heterozygotes
- multiple cysts involving both tubules and glomeruli

hydronephrosis
- marked hydronephrosis in 12% of heterozygotes but ureter is undilated

reproductive system phenotype

abnormal seminiferous tubule morphology
- sometimes cystic

abnormal male reproductive system morphology

abnormal prostate gland anterior lobe morphology
- sometimes cystic
- sometimes with abnormal lobulation

decreased litter size
- average litter from mating a wild-type C57BL/6 mouse with a C57BL/6 heterozygote yields only 1 heterozygous pup

reduced female fertility
- female heterozygotes are poorer breeders relative to wild-type females

abnormal fertility/fecundity

skeleton phenotype

short nasal bone
- in about 12% of individuals

short frontal bone
- in about 12% of individuals

vision/eye phenotype

abnormal anterior eye segment morphology
- variable anterior segment abnormalities in mutants 3-5 months of age

abnormal canal of Schlemm morphology
- small or absent

abnormal cornea morphology
- extracellular matrix (ECM) abnormalities are seen in the peripheral cornea, showing irregularly arranged collagen bundles

abnormal eye morphology
- Background Sensitivity - mutants on a C57BL/6J background exhibit variable anterior and posterior segment abnormalities that are reduced on a mixed C3Hf/HeA and C57BL/LiA background and are rarely seen in mutants on CAST/Ei, 129S6/SvEvTac, or BALB/cJ background

abnormal line of Schwalbe morphology
- displaced Schwalbe's line

abnormal retina morphology

corneal opacity
- some eyes exhibit scleralization (opacity) of the the peripheral cornea or diffuse corneal haze

iris hypoplasia
- iris is generally normal, however in some eyes, the iris is hypoplastic and malformed; occasionally the malformation is extensive involving both iris and ciliary body

abnormal retinal photoreceptor layer morphology
- photoreceptor layer typically appears normal, however there are foci of retinal dysplasia, characterized by rosette formation

anterior iris synechia
- abnormal iridocorneal attachments (anterior synechiae) of variable extent

thin retinal ganglion layer
- retinal ganglion cell layer on average contains about 50% the normal number of cells

thin retinal inner nuclear layer

abnormal iridocorneal angle
- abnormal in most eyes

anterior cortical cataract
- anterior subcapsular and cortical contaracts occur in most mutants

cataract

absent Schlemm's canal
- small or absent

opacity of vitreous body
- irregular white patches in the vitreous and dense vitreous haze in the majority of eyes

abnormal optic disk morphology
- abnormalities of the optic nerve head are frequently observed

abnormal placement of pupils
- pupils are often eccentrically located

abnormal vitreous body morphology
- dense network of small tortuous vessels throughout the vitreous that leak fluorescein, indicating compromised integrity of the vessels

absent optic nerve
- the optic nerve is sometimes absent

anterior subcapsular cataract
- anterior subcapsular and cortical contaracts occur in most mutants

decreased cornea thickness
- peripheral cornea is often thinner with neovascularization

microphthalmia
- frequency increased 3 fold

abnormal eye electrophysiology
- in some eyes, both a- and b-wave amplitude are reduced, due in part to poor pupillary dilation, with preferential loss of b-wave relative to a-wave

abnormal eye size

abnormal retinal blood vessel pattern
- the main retinal vessels branch close to the optic nerve and are irregularly arranged compared to wild-type

absent trabecular meshwork
- hypoplastic or absent trabecular meshwork that appears compressed and stalled in development

anophthalmia
- frequency increased 3 fold

hypoplastic trabecular meshwork
- hypoplastic or absent trabecular meshwork that appears compressed and stalled in development

irregularly shaped pupil

retinal detachment
- about 25% of heterozygotes exhibit retinal detachment as early as P30, with increased incidence as mice age

abnormal optic nerve morphology
- optic nerve abnormalities range from normal to absent, and are most often severely abnormal consisting of loose connective tissue, with absence of neural tissue

abnormal posterior eye segment morphology
- variable posterior eye segment abnormalities

ocular hypertension
- mutants with severe drainage structure abnormalities over 80% or more of their angle's extent have elevated intraocular pressure

persistence of hyaloid vascular system
- abnormal persistence of the anterior hyaloid vessels
- posterior hyaloid vessels persist throughout the 17 month period studied and increase in number and size beyond that normally seen at birth

cardiovascular system phenotype

abnormal retinal blood vessel pattern
- the main retinal vessels branch close to the optic nerve and are irregularly arranged compared to wild-type

craniofacial phenotype

short nasal bone
- in about 12% of individuals

short frontal bone
- in about 12% of individuals

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
B6.129S2-Bmp4

embryo phenotype

abnormal extraembryonic mesoderm development
- hypoplastic extraembryonic mesoderm: small amount can be distinguished

abnormal visceral yolk sac morphology

absent visceral yolk sac blood islands
- there is a relative paucity of blood islands containing red blood cells in the visceral yolk sac

failure of primitive streak formation
- absence of organized primitive streak

hematopoietic system phenotype

decreased erythrocyte cell number
- few red blood cells are found in the heart, dorsal aorta, and vessels of an E8.5 embryo

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance
- none survive beyond the egg cylinder stage

References

Winnier G; Blessing M; Labosky PA; Hogan BL. 1995. Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse. Genes Dev 9(17):2105-16PubMed: 7657163 MGI: J:28717

Additional References

Dunn NR; Winnier GE; Hargett LK; Schrick JJ; Fogo AB; Hogan BL. 1997. Haploinsufficient phenotypes in Bmp4 heterozygous null mice and modification by mutations in Gli3 and Alx4. Dev Biol 188(2):235-47PubMed: 9268572 MGI: J:42445
Paine-Saunders S; Viviano BL; Zupicich J; Skarnes WC; Saunders S. 2000. glypican-3 controls cellular responses to Bmp4 in limb patterning and skeletal development. Dev Biol 225(1):179-87PubMed: 10964473 MGI: J:64330

Additional - Bmp4^tm1Blh related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Bmp4
- Genotyping resources and troubleshooting
Breeding Considerations

The strain is maintained by breeding heterozygous mice to normal wildtype siblings or C57BL/6 inbred mice. Heterozygous breeder pairs are supplied. Female heterozygous mice appear to be poorer breeders than wildtype females and often have an inner ear defect that may cause the mice to spin. Heterozygous breeders are selected for the least severe expression of the inner ear defect. Expected coat color from breeding:Black
- Additional Breeding and Husbandry Support
Appearance
- black
  Related Genotype: a/a
Citation
When using the B6.129S2-Bmp4^tm1Blh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002612 in your Materials and Methods section.

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Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

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Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Bmp4<tm1Blh>

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Additional Information

Selected References

Bmp4tm1Blh

Allele Symbol: Bmp4tm1Blh

Disease Terms

Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Bmp4tm1Blh related

Mammalian Phenotype Terms by Genotype

Genotype: Bmp4tm1Blh/Bmp4tm1Blhinvolves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss

cardiovascular system phenotype

embryo phenotype

Genotype: Bmp4tm1Blh/Bmp4tm1Blhinvolves: 129S2/SvPas * C57BL/6

embryo phenotype

growth/size/body region phenotype

mortality/aging

Genotype: Bmp4tm1Blh/Bmp4+involves: 129S2/SvPas * BALB/cJ

vision/eye phenotype

Genotype: Bmp4tm1Blh/Bmp4+involves: 129S2/SvPas * C57BL/6 * CBA

behavior/neurological phenotype

cellular phenotype

hearing/vestibular/ear phenotype

reproductive system phenotype

Genotype: Bmp4tm1Blh/Bmp4+involves: 129S2/SvPas * 129S6/SvEvTac

cellular phenotype

reproductive system phenotype

vision/eye phenotype

Genotype: Bmp4tm1Blh/Bmp4tm1Blhinvolves: 129S2/SvPas * Black Swiss

cellular phenotype

reproductive system phenotype

vision/eye phenotype

embryo phenotype

endocrine/exocrine gland phenotype

growth/size/body region phenotype

mortality/aging

nervous system phenotype

Genotype: Bmp4tm1Blh/Bmp4tm1Blhinvolves: 129S2/SvPas * C57BL/6 * CBA

cellular phenotype

embryo phenotype

growth/size/body region phenotype

mortality/aging

reproductive system phenotype

Genotype: Bmp4tm1Blh/Bmp4tm1Blh129S2/SvPas-Bmp4

embryo phenotype

mortality/aging

Genotype: Bmp4tm1Blh/Bmp4tm1Blhinvolves: 129S2/SvPas * ICR

embryo phenotype

growth/size/body region phenotype

mortality/aging

Genotype: Bmp4tm1Blh/Bmp4+involves: 129S2/SvPas * C57BL/6

behavior/neurological phenotype

renal/urinary system phenotype

growth/size/body region phenotype

hearing/vestibular/ear phenotype

homeostasis/metabolism phenotype

mammalian phenotype

Genotype: Bmp4tm1Blh/Bmp4+involves: 129S2/SvPas * C3Hf/HeA * C57BL/LiA

vision/eye phenotype

Genotype: Bmp4tm1Blh/Bmp4+involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss

mammalian phenotype

Genotype: Bmp4tm1Blh/Bmp4+involves: 129S2/SvPas * CAST/Ei

vision/eye phenotype

Genotype: Bmp4tm1Blh/Bmp4tm4Blhinvolves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss * C57BL/6 * SJL

hearing/vestibular/ear phenotype

Genotype: Bmp4tm1Blh/Bmp4+B6.129S2-Bmp4

behavior/neurological phenotype

endocrine/exocrine gland phenotype

hearing/vestibular/ear phenotype

limbs/digits/tail phenotype

mortality/aging

nervous system phenotype

renal/urinary system phenotype

Bmp4^tm1Blh

Allele Symbol: Bmp4^tm1Blh

Genotype: Bmp4^tm1Blh related

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * C57BL/6

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * BALB/cJ

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * C57BL/6 * CBA

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * 129S6/SvEvTac

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * Black Swiss

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * C57BL/6 * CBA

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
129S2/SvPas-Bmp4

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
involves: 129S2/SvPas * ICR

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * C57BL/6

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * C3Hf/HeA * C57BL/LiA

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss

Genotype: Bmp4^tm1Blh/Bmp4⁺
involves: 129S2/SvPas * CAST/Ei

Genotype: Bmp4^tm1Blh/Bmp4^tm4Blh
involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss * C57BL/6 * SJL

Genotype: Bmp4^tm1Blh/Bmp4⁺
B6.129S2-Bmp4

Genotype: Bmp4^tm1Blh/Bmp4^tm1Blh
B6.129S2-Bmp4

Additional - Bmp4^tm1Blh related