STOCK Ghrhr^lit Prkdc^scid/Bm

Stock number: 002577
Research areas: Research Tools

Description

This strain has been discontinued, please refer to Stock No. 010816

Detailed description

Mice homozygous for the severe combined immune deficiency spontaneous mutation (Prkdc^scid, commonly referred to as scid) are characterized by an absence of functional T cells and B cells, lymphopenia, hypogammaglobulinemia, and a normal hematopoietic microenvironment. Normal antigen-presenting cell, myeloid and NK cell functions are strain dependent. scid mice carry a DNA repair defect and a defect in the rearrangement of genes that code for antigen-specific receptors on lymphocytes. Most homozygotes have no detectable IgM, IgG1, IgG2a, IgG2b, IgG3, or IgA. Thymus, lymph nodes, and splenic follicles are virtually devoid of lymphocytes. scid mice accept allogeneic and xenogeneic grafts making them an ideal model for cell transfer experiments. Some scid mice will spontaneously develop partial immune reactivity. scid mice that have serum Ig levels greater than 1 ug/ml are considered "leaky." scid leakiness is highly strain dependent, increases with age, and is higher in mice housed under non SPF conditions. In general, scid leakiness is high on the C57BL/6J and BALB/cBy genetic backgrounds, low on the C3H/HeJ background, and even lower on the NOD/LtSz background. In addition to the effects of the scid mutation, Ghrhr^litPrkdc^scid /Bm mice are deficient in growth hormone and IGF1 while Ghrh^hpgPrkdc^scid/Bm mice (Stock No. 002038) are deficient in gonadotropins and gonodal steroids. These two double mutant strains are useful for determining endocrine dependence of grafted cells and tissues.

Development

C57BL/6J-Ghrhr^lit/Ghrhr^lit x C.B-17-Prkdc^scid/Prkdc^scid offspring were sibling mated. The strain was made homozygous for Prkdc^scid and kept segregating for Ghrhr^lit and the sibling mating continued to the present.