JAX Mouse Strain Datasheet - 002549

STOCK Tg^cog/J

Stock No:: 002549 |; congenital goiter

Cryo Recovery

Overview

Also Known As: congenital goiter

These mice carry the spontaneous Tg^cog mutation and are characterized by development of goiters and smaller overall size.

Genetic overview

Genetic Background	Generation

Tg^cog

Allele Type	Gene Symbol	Gene Name
Spontaneous	Tg	thyroglobulin

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Research Applications

Cell Biology Research
Endocrine Deficiency Research
Internal/Organ Research

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

The Tgn^cog mutation causes the development of goiters due to failed processing of thyroglobulin. Homozygotes are smaller in overall size by 15 days of age. They have an increase in growth rate at the time of weaning but generally do not attain comparable size with their wildtype littermates. Increased thyroidal volume is apparent at embryonic day 18 and continues enlarging to an average of 5 fold higher than normal at 8 weeks of age and 20 fold normal at 10 months of age. In addition to decreased serum T3 and T4 levels, homozygotes have increased serum thyroid stimulating hormone levels, reduced levels of serum IGFBP-3, IGFBP-4, and IGFBP-2, mild anemia, and hypomyelination restricted to the cerebrum. Tgn^cog is an outwardly recessive mutation but microdissection reveals a heterozygous phenotype as well. Thyrofollicular cells of heterozygotes have swollen protein-containing vesicles similar to but more moderate than those found in homozygotes. Thyroid extracts from homozygotes have an increased percent of protein and heterozygotes have an intermediate percent of protein relative to wild type siblings. (Beamer et al., 1987; Adkison et al., 1990; Sugisaki et al., 1991, 1992, and 1993; Kim et al., 1996 and1998.)

Development

The congenital goiter mutation arose spontaneously in the AKR/J strain (then at generation F131) at The Jackson Laboratory. The mutation was maintained via sibling mating for 9 generations then backcrossed once to AKR/J then sibling mated for 4 generations before being backcrossed to BALB/cBy. A single male at N1F4 was bred to a BALB/cBy female, their heterozygous offspring sibling mated to generate a homozygous N1F1 female that was backcrossed to a BALB/cBy male. Their heterozygous N2 offspring were sibling mated to generate a homozygous male that was backcrossed to a BALB/cBy female. The backcross-intercross breeding scheme was continued from this point on using female BALB/cBy and male homozygotes in the backcross generations until the congenic strain reached N10. This strain was then maintained via sibling mating and in 1995 homozygous males at generation N10F21 were bred with C57BL/6J females to generate embryos for cryopreservation.

Selected References

Beamer WG; Maltais LJ; DeBaets MH; Eicher EM. 1987. Inherited congenital goiter in mice. Endocrinology 120(2):838-40. PubMed: 3803305 MGI: J:8567

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Genetics

Tg^cog

Allele Symbol: Tg^cog

Allele Name	congenital goiter
Allele Type	Spontaneous
Allele Synonym(s)	Tgn^cog; cog
Gene Symbol and Name	Tg, thyroglobulin
Gene Synonym(s)	AITD3; TGN; Tgn; Tgn; cog; congenital goiter
Strain of Origin	AKR/J
Chromosome	15
Molecular Note	The mutation is a C to T transition yielding a leucine to proline change at residue 2263. This falls within the acetylcholinesterase domain and impacts protein conformation. This conformational mutation is temperature sensitive; there is an increase in the level of TGN secreted from mutant thyrocytes at 31 degrees relative to the level secreted at 37 degrees, which is below the threshold of detection by PAGE. A small amount of functional TGN is processed in homozygous mice and serum triiodothyroinine and tetraiodothyroinine are found, albeit at vastly reduced levels.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Tg^cog related

Cell Biology Research
- Post-translational Processing
- Protein Processing
Endocrine Deficiency Research
- Thyroid Defects
Internal/Organ Research
- Thyroid Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tg^cog/Tg^cog
AKR/J-Tg^cog

endocrine/exocrine gland phenotype

abnormal thyroid follicle morphology
- obliteration of most follicular lumens
- small numbers of lymphocytes in degenerating follicles

abnormal thyroid follicular cell morphology
- hypertrophy and hyperplasia of thyrofollicular cells

enlarged thyroid gland
- reduced when diet supplemented with desiccated thyroid powder

growth/size/body region phenotype

disproportionate dwarf
- evident at day 15 postnatal
- repaired when diet supplemented with desiccated thyroid powder

increased growth rate
- evident between 4 and 7 weeks of age

homeostasis/metabolism phenotype

decreased circulating thyroxine level
- serum thyroxine is significantly reduced

Genotype: Tg^cog/Tg^cog
involves: AKR/J * C57BL/6By

endocrine/exocrine gland phenotype

enlarged thyroid gland
- hypertrophy of thyrofollicular cells

growth/size/body region phenotype

decreased body weight
- evident at day 15 postnatal
- evident by 15 days postnatal
- repaired when diet at weaning was supplemented with desiccated thyroid powder

hematopoietic system phenotype

anemia

decreased hematocrit

homeostasis/metabolism phenotype

decreased circulating thyroxine level

decreased circulating triiodothyronine level

increased circulating thyroid-stimulating hormone level

nervous system phenotype

abnormal myelination
- hypomyelination, restricted to cerebrum

decreased brain weight
- brain stem weight equivalent to littermate controls
- cerebrum and cerebellum weighed significantly less than littermate controls

References

Beamer WG; Maltais LJ; DeBaets MH; Eicher EM. 1987. Inherited congenital goiter in mice. Endocrinology 120(2):838-40. PubMed: 3803305 MGI: J:8567

Additional References

Adkison LR; Taylor S; Beamer WG. 1990. Mutant gene-induced disorders of structure, function and thyroglobulin synthesis in congenital goitre (cog/cog) in mice. J Endocrinol 126(1):51-8. PubMed: 1696305 MGI: J:46324
Mayerhofer A; Amador AG; Beamer WG; Bartke A. 1988. Ultrastructural aspects of the goiter in cog/cog mice. J Hered 79(3):200-3. PubMed: 3392390 MGI: J:24038
Sugisaki T; Beamer WG; Noguchi T. 1992. Microcephalic cerebrum with hypomyelination in the congenital goiter mouse (cog). Neurochem Res 17(10):1037-40. PubMed: 1508304 MGI: J:3079

Additional - Tg^cog related

Beamer WG; Coleman DL. 1982. [Adipose storage deficiency (asd)]. Mouse News Lett 67:21. MGI: J:13923
Kim PS; Hossain SA; Park YN; Lee I; Yoo SE; Arvan P. 1998. A single amino acid change in the acetylcholinesterase-like domain of thyroglobulin causes congenital goiter with hypothyroidism in the cog/cog mouse: a model of human endoplasmic reticulum storage diseases. Proc Natl Acad Sci U S A 95(17):9909-13. PubMed: 9707574 MGI: J:49474
Kim PS; Kwon OY; Arvan P. 1996. An endoplasmic reticulum storage disease causing congenital goiter with hypothyroidism. J Cell Biol 133(3):517-27. PubMed: 8636228 MGI: J:156005
Mayerhofer A; Amador AG; Beamer WG; Bartke A. 1988. Ultrastructural aspects of the goiter in cog/cog mice. J Hered 79(3):200-3. PubMed: 3392390 MGI: J:24038
Sugisaki T; Beamer WG; Noguchi T. 1992. Microcephalic cerebrum with hypomyelination in the congenital goiter mouse (cog). Neurochem Res 17(10):1037-40. PubMed: 1508304 MGI: J:3079

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Carrier for Tg<cog>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Licensing Information

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Also Known As: congenital goiter

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Selected References

Tgcog

Allele Symbol: Tgcog

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Tgcog related

Mammalian Phenotype Terms by Genotype

Genotype: Tgcog/TgcogAKR/J-Tgcog

endocrine/exocrine gland phenotype

growth/size/body region phenotype

homeostasis/metabolism phenotype

Genotype: Tgcog/Tgcoginvolves: AKR/J * C57BL/6By

endocrine/exocrine gland phenotype

growth/size/body region phenotype

hematopoietic system phenotype

homeostasis/metabolism phenotype

nervous system phenotype

References

Additional References

Additional - Tgcog related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Tg^cog

Allele Symbol: Tg^cog

Genotype: Tg^cog related

Genotype: Tg^cog/Tg^cog
AKR/J-Tg^cog

Genotype: Tg^cog/Tg^cog
involves: AKR/J * C57BL/6By

Additional - Tg^cog related