Mice homozygous for the Trp53tm1Tyj mutation show no visible phenotype but most develop tumors (principally lymphomas and sarcomas) at 3-6 months of age. Heterozygous mice develop tumors at about 10 months of age. These mice model some of the features of human Li-Fraumeni syndrome, a form of familial breast cancer with mutations in TRP53. Homozygous mice may produce a litter before succumbing to tumors.
The -25; mutant strain was developed in the laboratory of Dr. Tyler Jacks at the Center for Cancer Research at the Massachusetts Institute of Technology. The 129-derived D3 ES cell line was used. The C3H/HeOuJ strain was produced by backcrossing the Trp53tm1Tyj mutation to C3H/HeOuJ mice. As of February 1996 this strain had been backcrossed five times (N5). It will continue to be backcrossed to C3H/HeOuJ mice and will be fixed at N10.
|Allele Name||targeted mutation 1, Tyler Jacks|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||p53-; p53delta; p53null; p53KO; Trp53-; Trp53KO|
|Gene Symbol and Name||Trp53, transformation related protein 53|
|Site of Expression||Normal Trp53 expression is widespread.|
|Strain of Origin||129S2/SvPas|
|General Note||This mutant allele was produced by a targeted neo insertion into the Trp53 locus. Homozygotes show no visible phenotype but develop tumors at 3-6 months of age. Heterozygotes develop tumors at 10 months of age. These mice model some of the features of human Li-Fraumeni syndrome (OMIM 151623), a form of familial breast cancer with mutations in TRP53 (J:16022)(J:16023) A specific human mutation found in hepatocellular carcinomas caused by hepatitis B infection or by aflatoxin exposure has been created in a mouse model, resulting in a similar gene product (J:27363).|
|Molecular Note||A neomycin cassette replaced 40% of the coding sequences beginning with exon 2 (upstream of the translation start site) and extending into exon 6.|
|Mutations Made By|| |
Dr. Tyler Jacks, Massachusetts Institute of Technology
This Trp53tm1Tyj strain is maintained by mating heterozygous mice to normal wildtype siblings. Heterozygous mice and normal wildtype siblings may be ordered.
When using the p53Δ mouse strain in a publication, please cite the originating article(s) and include JAX stock #002547 in your Materials and Methods section.