B6;129-Adra2b^tm1Gsb/J

Stock number: 002511
Product name: Adra2b KO
Research areas: Cardiovascular Research

Detailed description

Homozygotes are viable and fertile. They exhibit an absence of alpha2-mediated peripheral vasoconstriction.

Development

This strain is maintained by mating homozygotes.

Allele

Symbol: Adra2b^tm1Gsb
Name: targeted mutation 1, Gregory S Barsh
MGI accession ID: MGI:1857118
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Adra2b
Marker name: adrenergic receptor, alpha 2b
Chromosome: 2
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A neomycin selection cassette was inserted into the coding sequences, placing a premature stop codon before the sequences encoding the fifth transmembrane domain. Western blot analysis on kidney membrane extracts confirmed that the protein was not detectable in homozygous mice.