These plasminogen activator, tissue knock-out (Plattm1Mlg, also commonly referred to as tPA-) mice may be useful in fibrosis, neurovascular and other studies involving the widespread physiological effects of plasminogen activator.
Dr. Peter Carmeliet, University of Leuven
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Plat | plasminogen activator, tissue |
Homozygotes develop normally, are fertile and have a normal life span. There are no histological abnormalities. Pulmonary clot lysis is 21% that of normal wildtype siblings. Endotoxin induced venous thrombosis is increased over that seen in normal wildtype siblings. Fibrin dissolution by PLAT-deficient macrophages is unaffected.
This targeted mutant was made in the laboratories of Dr. Richard Mulligan of the Whitehead Institute for Biomedical Sciences, Cambridge Massachusetts and of Dr. Peter Carmellet at the Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium. A targeting vector was used which resulted in the neomycin-resistant gene (neo) replacing genomic sequences encoding most of the kringle-2 domain and part of the proteinase domain, including the essential histidine-326 residue. Targeting was done in 129S2/SvPas derived D3 ES cells. The mice were backcrossed to C57BL/6 for 8 generations.
Allele Name | targeted mutation 1, Richard C Mulligan |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Plattm1; t-PA-; tPA- |
Gene Symbol and Name | Plat, plasminogen activator, tissue |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 8 |
Molecular Note | The gene was disrupted using neomycin resistance cassette. The vector replaced genomic sequences encoding most of the kringle-2 domain and part of the proteinase domain, including the catalytically essential histidine residue at position 326. Targeting was confirmed by the absence of gene specific mRNA and immunoreactivity. |
Mutations Made By | Dr. Peter Carmeliet, University of Leuven |
When maintaining a live colony, these mice can be bred as homozygotes. the expected coat color from breeding is black.
When using the t-PA- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002508 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Plat<tm1Mlg> |
Frozen Mouse Embryo | B6.129S2-Plat<tm1Mlg>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Plat<tm1Mlg>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Plat<tm1Mlg>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S2-Plat<tm1Mlg>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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