These Cga knock-out mice exhibit severe hypothyroidism resulting in dwarfism. They are suitable for use in applications related to the study of the role of pituitary thyrotropin (TSH) and gonadotropins (LH and FSH) in thyroid and gonadal development
Dr. Sally A. Camper, University of Michigan Medical School
Mice homozygous for the Cgatm1Sac targeted mutation are viable but both sexes are infertile. They lack TSH, LH, and FSH. Homozygous mutant mice are hypogonadal and exhibit severe hypothyroidism resulting in dwarfism. Development of the thyroid gland was arrested in late gestation. However, gonadotropin releasing hormone (GNRH) neuron migration, development of secondary sex organs, and fetal and neonatal gonadal development are normal. Mice heterozygous for the Cgatm1Sac targeted mutation appear normal.
|Allele Name||targeted mutation 1, Sally A Camper|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||TSHalpha -|
|Gene Symbol and Name||Cga, glycoprotein hormones, alpha subunit|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin resistance gene was inserted into the third exon. In situ hybridization and RT-PCR experiments confirmed that the transcript was not present in pituitary of homozygous mice (data not shown).|
|Mutations Made By|| |
Dr. Sally Camper, University of Michigan Medical School
When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. Homozygous mice are viable but both sexes are infertile. The expected coat color from breeding is black.
When using the α-subunit- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002494 in your Materials and Methods section.