These Cga knock-out mice exhibit severe hypothyroidism resulting in dwarfism. They are suitable for use in applications related to the study of the role of pituitary thyrotropin (TSH) and gonadotropins (LH and FSH) in thyroid and gonadal development
Dr. Sally A. Camper, University of Michigan Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cga | glycoprotein hormones, alpha subunit |
Mice homozygous for the Cgatm1Sac targeted mutation are viable but both sexes are infertile. They lack TSH, LH, and FSH. Homozygous mutant mice are hypogonadal and exhibit severe hypothyroidism resulting in dwarfism. Development of the thyroid gland was arrested in late gestation. However, gonadotropin releasing hormone (GNRH) neuron migration, development of secondary sex organs, and fetal and neonatal gonadal development are normal. Mice heterozygous for the Cgatm1Sac targeted mutation appear normal.
Allele Name | targeted mutation 1, Sally A Camper |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | TSHalpha - |
Gene Symbol and Name | Cga, glycoprotein hormones, alpha subunit |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 4 |
Molecular Note | A neomycin resistance gene was inserted into the third exon. In situ hybridization and RT-PCR experiments confirmed that the transcript was not present in pituitary of homozygous mice (data not shown). |
Mutations Made By | Dr. Sally Camper, University of Michigan Medical School |
When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. Homozygous mice are viable but both sexes are infertile. The expected coat color from breeding is black.
When using the α-subunit- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002494 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Cga<tm1Sac> |
Frozen Mouse Embryo | B6;129S2-Cga<tm1Sac>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Cga<tm1Sac>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Cga<tm1Sac>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S2-Cga<tm1Sac>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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