B6;129S2-Cga^tm1Sac/J

Stock number: 002494
Product name: α-subunit-
Research areas: Developmental Biology Research, Endocrine Deficiency Research, Internal/Organ Research, Reproductive Biology Research

Description

These Cga knock-out mice exhibit severe hypothyroidism resulting in dwarfism. They are suitable for use in applications related to the study of the role of pituitary thyrotropin (TSH) and gonadotropins (LH and FSH) in thyroid and gonadal development

Detailed description

Mice homozygous for the Cga^tm1Sac targeted mutation are viable but both sexes are infertile. They lack TSH, LH, and FSH. Homozygous mutant mice are hypogonadal and exhibit severe hypothyroidism resulting in dwarfism. Development of the thyroid gland was arrested in late gestation. However, gonadotropin releasing hormone (GNRH) neuron migration, development of secondary sex organs, and fetal and neonatal gonadal development are normal. Mice heterozygous for the Cga^tm1Sac targeted mutation appear normal.

Allele

Symbol: Cga^tm1Sac
Name: targeted mutation 1, Sally A Camper
MGI accession ID: MGI:1857152
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cga
Marker name: glycoprotein hormones, alpha subunit
Chromosome: 4
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance gene was inserted into the third exon. In situ hybridization and RT-PCR experiments confirmed that the transcript was not present in pituitary of homozygous mice (data not shown).