Homozygotes have major defects in both pattern and myotomal formation.
This strain was developed in the lab of Dr. Barbara Wold at the California Institute of Technology. Coding sequence of the myogenic factor 6 (Myf6) gene was replaced with a neomycin resistance cassette. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ7 embryonic Stem cells (ES cells). Correctly targeted ES cells was injected into blastocysts, and the resulting mice were bred to C57BL/6J inbred mice (Stock No. 000664).
|Allele Name||targeted mutation 1, Barbara Wold|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Myf6, myogenic factor 6|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|General Note||Studies have shown that Myf6 may regulate Myf5 expression by a cis-acting mechanism and that the skeletal abnormalities seen in Myf6 homozygotes may result from a decrease or lack of Myf5 expression (J:29279, J:42453). This allele (Myf6tm1Wb) exhibits normal Myf5 expression at E8 but by E10, expression is reduced (J:29279).|
|Molecular Note||Replacement of DNA sequences encoding codons 40-173 with a neomycin cassette. This mutation eliminates the helix-loop-helix domain of the protein. Expression of Myf5 is seen at E8, however by E10, it is lower and remains lower.|
|Mutations Made By|| |
Dr. Barbara Wold, California Institute of Technology
The strain is maintained by mating heterozygotes and normal wild type siblings. 5/27/97 keb: We are outcrossing to B6 each generation since homozygous mice are lethal at birth.
When using the B6.129S1-Myf6tm1Wb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002475 in your Materials and Methods section.