Overview

Also Known As:129S1

129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.

Genetic overview

Genetic Background	Generation
	Contact Technical Support (2019-04-23 00:00:00)

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Research Applications

Reproductive Biology Research
Cancer Research
Research Tools
Neurobiology Research

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Base Price

Starting at:

$44.09 Domestic price for female 3-week

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Details

Detailed Description

Historically, the 129 inbred mice are known for the high incidence of spontaneous testicular teratomas, though the incidence differs between substrains (1-3% in 129 parental substrains; 30% in teratoma substrains.) More recently, 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. There is major genetic variation within the 129 "family", which has led to an update of the nomenclature and a division of the substrains into three major groups: parental substrains (129P), steel substrains (129S) and "teratoma" substrains (129T). Investigators using 129 substrains for targeted mutagenesis should be careful in the selection of the appropriate 129 substrain to match the embryonic stem cell line. For a complete history of the numerous 129 substrains, see Simpson, et al., 1997.

In 2019-2020, researchers at The Jackson Laboratory discovered this inbred strain contains the Trem2^S148E allele - a naturally occurring variant at position 48351151-48351152 on Chr 17 (rs108080490 and rs107649577; Ensembl GRCm38.p6). This TC to GA transition results in a serine to glutamic acid substitution at amino acid 148 (S148E).

Development

129S1/SvImJ was developed to serve as a control inbred strain for many of the steel-derived ES cell lines (e.g. W9.5 and CJ7). SSLP marker analysis indicates that 129S1/SvImJ is identical to 129S1/Sv +^Oca2-p +^Tyr-c Kitl^Sl-J/+ except for the region surrounding the Kitl gene on Chr 10. 129S1/SvImJ was derived from 129S1/Sv-+^Oca2-p +^Tyr-c Kitl^Sl-J/+ (Stock No.
000090). The steel-Jackson mutation (Kitl^Sl-J, formerly Mgf^Sl-J), is segregating in Stock No. 000090. Kitl^Sl-J was removed in 1995, at F26, by selective breeding to produce 129/Sv-+^Oca2-p +^Tyr-c +^Kitl-SlJ (Stock No. 002448, see Simpson et al., 1997). This name was later shortened to simply 129/SvImJ. Subsequently designated 129S3/SvImJ (Festing et al., 1999), this strain was renamed 129S1/SvImJ in February, 2001 to emphasize its relationship to Stock No. 000090.

Selected References

Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298
Timmermans S; Van Montagu M; Libert C. 2017. Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains. Proc Natl Acad Sci U S A 114(34):9158-9163PubMed: 28784771 MGI: J:244295

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Genetics

Disc1^del

Allele Symbol: Disc1^del

Allele Name	deletion
Allele Type	Spontaneous
Allele Synonym(s)	Disc1^129S6; Disc1^delta6
Gene Symbol and Name	Disc1, disrupted in schizophrenia 1
Gene Synonym(s)
Strain of Origin	various
Chromosome	8
General Note	This deletion appears in multiple strains of the 129 superfamily, 101/RI, BTBR T⁺ tf/J, LP/J, FVB/NJ, SJL/J, SWR/J and DDY/JclSidSeyFrkJ (J:111837, J:195189).
Molecular Note	A 25 bp deletion in exon 6 causes a frame shift in the reading frame, resulting in 13 novel amino acids and a premature stop codon in exon 7.

Cox7a2l^l

Allele Symbol: Cox7a2l^l

Allele Name	long
Allele Type	Not Applicable (Not Specified)
Allele Synonym(s)	SCAF1¹¹³
Gene Symbol and Name	Cox7a2l, cytochrome c oxidase subunit 7A2 like
Gene Synonym(s)
Strain of Origin	multiple strains
Chromosome	17
General Note	Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T⁺ Itpr3^tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ.
Molecular Note	This allele encodes the long isoform with 113 amino acids. It is found in 129S2/SvPasCrl, CBA/CaOlaHsd, Hsd:ICR, and NZB/OlaHsd.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Reproductive Biology Research
- Gonadal Tumors
  - testicular teratomas
Cancer Research
- Increased Tumor Incidence
  - Gonadal Tumors: testicular teratomas
  - Gonadal Tumors
Research Tools
- General Purpose
- Genetics Research
  - Mutagenesis and Transgenesis: Production of Targeted Mutations (Knockouts)
  - Mutagenesis and Transgenesis
- Infectious Disease
  - Anthrax
Neurobiology Research
- Neurodevelopmental Defects
  - callosal agenesis, incomplete penetrance

Mammalian Phenotype Terms by Genotype

Phenotype Information

References

Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11PubMed: 15081119 MGI: J:89298
Timmermans S; Van Montagu M; Libert C. 2017. Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains. Proc Natl Acad Sci U S A 114(34):9158-9163PubMed: 28784771 MGI: J:244295

Additional References

Clapcote SJ; Roder JC. 2006. Deletion polymorphism of disc1 is common to all 129 mouse substrains: implications for gene-targeting studies of brain function. Genetics 173(4):2407-10PubMed: 16751659 MGI: J:111837
Festing MF; Simpson EM; Davisson MT; Mobraaten LE. 1999. Revised nomenclature for strain 129 mice. Mamm Genome 10(8):836PubMed: 10430671 MGI: J:56500
Keane TM; Goodstadt L; Danecek P; White MA; Wong K; Yalcin B; Heger A; Agam A; Slater G; Goodson M; Furlotte NA; Eskin E; Nellaker C; Whitley H; Cleak J; Janowitz D; Hernandez-Pliego P; Edwards A; Belgard TG; Oliver PL; McIntyre RE; Bhomra A; Nicod J; Gan X; Yuan W; van der Weyden L; Steward CA; Bala S; Stalker J; Mott R; Durbin R; Jackson IJ; Czechanski A; Guerra-Assuncao JA; Donahue LR; Reinholdt LG; Payseur BA; Ponting CP; Birney E; Flint J; Adams DJ. 2011. Mouse genomic variation and its effect on phenotypes and gene regulation. Nature 477(7364):289-94PubMed: 21921910 MGI: J:177037
Moy SS; Nadler JJ; Young NB; Perez A; Holloway LP; Barbaro RP; Barbaro JR; Wilson LM; Threadgill DW; Lauder JM; Magnuson TR; Crawley JN. 2007. Mouse behavioral tasks relevant to autism: phenotypes of 10 inbred strains. Behav Brain Res 176(1):4-20PubMed: 16971002 MGI: J:138682
Rohan RM; Fernandez A; Udagawa T; Yuan J; D'Amato RJ. 2000. Genetic heterogeneity of angiogenesis in mice. FASEB J 14(7):871-6PubMed: 10783140 MGI: J:61808
Simpson EM; Linder CC; Sargent EE; Davisson MT; Mobraaten LE; Sharp JJ. 1997. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat Genet 16(1):19-27PubMed: 9140391 MGI: J:40222
Stevens LC. 1973. A new inbred subline of mice (129-terSv) with a high incidence of spontaneous congenital testicular teratomas. J Natl Cancer Inst 50(1):235-42PubMed: 4692863 MGI: J:29502
Threadgill DW; Yee D; Matin A; Nadeau JH; Magnuson T. 1997. Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain. Mamm Genome 8(6):390-3PubMed: 9166580 MGI: J:40661
Wahlsten D; Metten P; Crabbe JC. 2003. Survey of 21 inbred mouse strains in two laboratories reveals that BTBR T/+ tf/tf has severely reduced hippocampal commissure and absent corpus callosum. Brain Res 971(1):47-54PubMed: 12691836 MGI: J:83159
Whitehead GS; Walker JK; Berman KG; Foster WM; Schwartz DA. 2003. Allergen-induced airway disease is mouse strain dependent. Am J Physiol Lung Cell Mol Physiol 285(1):L32-42PubMed: 12626335 MGI: J:84265
Zechel JL; MacLennan GT; Heaney JD; Nadeau JH. 2011. Spontaneous metastasis in mouse models of testicular germ-cell tumours. Int J Androl 34(4 Pt 2):e278-87PubMed: 21651572 MGI: J:235639

Additional - Cox7a2l^l related

Additional - Disc1^del related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Inbred mouse strains are maintained through sibling (sister x brother) matings; no genotyping required.
Breeding Considerations

This strain is a good breeder.

Wean-aged mice may show signs of barbering that include whisker picking. Affected mice typically regrow whiskers within two weeks after weaning.
- Additional Breeding and Husbandry Support
Mating System
- Sibling x Sibling
Appearance
- white-bellied agouti
  Related Genotype: A^w/A^w
Citation
When using the 129S1 mouse strain in a publication, please include JAX stock #002448 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

MP14 (Maximum)

AX30 (Maximum)

Pricing & Availability

Readily Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Inbred, 1 pair minimum will be supplied

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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