129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.
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Historically, the 129 inbred mice are known for the high incidence of spontaneous testicular teratomas, though the incidence differs between substrains.(1-3% in 129 parental substrains; 30% in teratoma substrains.) More recently, 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. There is major genetic variation within the 129 "family", which has led to an update of the nomenclature and a division of the substrains into three major groups: parental substrains (129P), steel substrains (129S) and "teratoma" substrains (129T). Investigators using 129 substrains for targeted mutagenesis should be careful in the selection of the appropriate 129 substrain to match the embryonic stem cell line. For a complete history of the numerous 129 substrains, see Simpson, et al., 1997.
In 2019-2020, researchers at The Jackson Laboratory discovered this inbred strain contains the Trem2S148E allele - a naturally occurring variant at position 48351151-48351152 on Chr 17 (rs108080490 and rs107649577; Ensembl GRCm38.p6). This TC to GA transition results in a serine to glutamic acid substitution at amino acid 148 (S148E).
129S1/SvImJ was developed to serve as a control inbred strain for many of the steel-derived ES cell lines (e.g. W9.5 and CJ7). SSLP marker analysis indicates that 129S1/SvImJ is identical to 129S1/Sv +Oca2-p +Tyr-c KitlSl-J/+ except for the region surrounding the Kitl gene on Chr 10. 129S1/SvImJ was derived from 129S1/Sv-+Oca2-p +Tyr-c KitlSl-J/+ (Stock No.
000090). The steel-Jackson mutation (KitlSl-J, formerly MgfSl-J), is segregating in Stock No. 000090. KitlSl-J was removed in 1995, at F26, by selective breeding to produce 129/Sv-+Oca2-p +Tyr-c +Kitl-SlJ (Stock No. 002448, see Simpson et al., 1997). This name was later shortened to simply 129/SvImJ. Subsequently designated 129S3/SvImJ (Festing et al., 1999), this strain was renamed 129S1/SvImJ in February, 2001 to emphasize its relationship to Stock No. 000090.
Allele Name | deletion |
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Allele Type | Spontaneous |
Allele Synonym(s) | Disc1129S6; Disc1delta6 |
Gene Symbol and Name | Disc1, disrupted in schizophrenia 1 |
Gene Synonym(s) | |
Strain of Origin | various |
Chromosome | 8 |
General Note | This deletion appears in multiple strains of the 129 superfamily, 101/RI, BTBR T+ tf/J, LP/J, FVB/NJ, SJL/J, SWR/J and DDY/JclSidSeyFrkJ (J:111837, J:195189). |
Molecular Note | A 25 bp deletion in exon 6 causes a frame shift in the reading frame, resulting in 13 novel amino acids and a premature stop codon in exon 7. |
Allele Name | long |
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Allele Type | Not Applicable (Not Specified) |
Allele Synonym(s) | SCAF1113 |
Gene Symbol and Name | Cox7a2l, cytochrome c oxidase subunit 7A2 like |
Gene Synonym(s) | |
Strain of Origin | multiple strains |
Chromosome | 17 |
General Note | Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T+ Itpr3tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ. |
Molecular Note | This allele encodes the long isoform with 113 amino acids. It is found in 129S2/SvPasCrl, CBA/CaOlaHsd, Hsd:ICR, and NZB/OlaHsd. |
When using the 129S1 mouse strain in a publication, please include JAX stock #002448 in your Materials and Methods section.
Service/Product | Description | Price |
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Inbred, 1 pair minimum will be supplied |
Mouse ES Cells | 129S1/SvImJ-AC677/GrsJ cells | $995.00 |
Mouse ES Cells | 129S1/SvImJ-AC677/GrsJ cells | $995.00 |
Mouse ES Cells | 129S1/SvImJ-AC677/GrsJ cells | $995.00 |
Mouse ES Cells | 129S1/SvImJ-AC677/GrsJ cells | $995.00 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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