These Cd4 knock-out mice exhibit a loss of helper T-cell activity and of other class-II restricted T-cell responses.
Dr. Dan R. Littman, New York University Medical Center
Mice homozygous for the Cd4tm1 targeted mutation have a significant block in CD4+ T cell development; 90% of their circulating T cells are CD8+. Homozygous mutant mice also show a Class II restricted deficit in helper T cell activity and other T cell responses.
A targeting vector was designed to insert neomycin resistance gene into exon 5. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric male progeny were then crossed with C57BL/6 females. These mice were backcrossed to C57BL/10 mice for at least 6 generations.
|Allele Name||targeted mutation 1, Dan R Littman|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD4-; Cd4tm1Drl|
|Gene Symbol and Name||Cd4, CD4 antigen|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin resistance gene was inserted into exon 5. No cell surface expression of the encoded protein was detected in T cells and thymocytes derived from homozygous mice (data not shown).|
|Mutations Made By|| |
Dr. Dan Littman, New York University Medical Center
While maintaining a live colony, these mice are maintained by mating homozygote siblings.
When using the CD4- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002447 in your Materials and Methods section.