B10.129S2(B6)-Cd4^tm1Litt/J

Stock number: 002447
Product name: CD4-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These Cd4 knock-out mice exhibit a loss of helper T-cell activity and of other class-II restricted T-cell responses.

Detailed description

Mice homozygous for the Cd4^tm1 targeted mutation have a significant block in CD4⁺ T cell development; 90% of their circulating T cells are CD8⁺. Homozygous mutant mice also show a Class II restricted deficit in helper T cell activity and other T cell responses.

Development

A targeting vector was designed to insert neomycin resistance gene into exon 5. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric male progeny were then crossed with C57BL/6 females. These mice were backcrossed to C57BL/10 mice for at least 6 generations.

Allele

Symbol: Cd4^tm1Litt
Name: targeted mutation 1, Dan R Littman
MGI accession ID: MGI:1857281
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd4
Marker name: CD4 antigen
Chromosome: 6
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance gene was inserted into exon 5. No cell surface expression of the encoded protein was detected in T cells and thymocytes derived from homozygous mice (data not shown).