These Cd4 knock-out mice exhibit a loss of helper T-cell activity and of other class-II restricted T-cell responses.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Cd4 | CD4 antigen |
Mice homozygous for the Cd4tm1 targeted mutation have a significant block in CD4+ T cell development; 90% of their circulating T cells are CD8+. Homozygous mutant mice also show a Class II restricted deficit in helper T cell activity and other T cell responses.
A targeting vector was designed to insert neomycin resistance gene into exon 5. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric male progeny were then crossed with C57BL/6 females. These mice were backcrossed to C57BL/10 mice for at least 6 generations.
Allele Name | targeted mutation 1, Dan R Littman |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD4-; Cd4tm1Drl |
Gene Symbol and Name | Cd4, CD4 antigen |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 6 |
Molecular Note | A neomycin resistance gene was inserted into exon 5. No cell surface expression of the encoded protein was detected in T cells and thymocytes derived from homozygous mice (data not shown). |
Mutations Made By | Dr. Dan Littman, New York University Medical Center |
While maintaining a live colony, these mice are maintained by mating homozygote siblings.
When using the CD4- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002447 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Cd4<tm1Litt>, 1 pair minimum |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.