These int-3 mice express a transgene containing the MMTV envelope and 3' LTR sequences and a truncated portion of the mouse Notch4 gene. Transgenic mice develop focal adenomas of the mammary and salivary glands and females fail to lactate.
Dr. Robert Callahan, National Institutes of Health
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Male mice transgenic for the Notch4 gene (previously called Int3) are sterile, and females fail to lactate. Mammary tissue of females does not develop completely, exhibiting dramatic inhibition of alveolar-lobular development and reduced penetration of the mammary fat pad by ductal epithelium. Glandular epithelia of tissues expressing the activated form of Notch4 generally display severe ductal hyperplasia. Salivary glands fail to differentiate completely. Male transgenic mice exhibit severe epididymal hyperplasia, which is thought to be the cause of their sterility. Both male and female mice develop focal adenomas of the mammary and salivary glands.
Transgenic mice were generated using a 7.5 kb genomic DNA fragment containing the 3' portion of the mouse Notch4 gene under the influence of the Czech II MMTV LTR. FVB/N embryos were microinjected. The FF1 line was established by superovulating and mating the female founder to a nontransgenic FVB male, isolating single-cell embryos, and transferring them to a pseudopregnant CD1 mouse. Thereafter, mice newly born to subsequent FF1 Notch4 transgenic mice were routinely moved to foster mothers.
Expressed Gene | Notch4, notch 4, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 3, Robert Callahan |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | FF1 int-3; FVB-3; MMTV LTR-INT3; MMTV-int-3; TgN(MMTVInt3)3Rnc |
Gene Symbol and Name | Tg(MMTV-Notch4)3Rnc, transgene insertion 3, Robert Callahan |
Gene Synonym(s) | |
Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV |
Expressed Gene | Notch4, notch 4, mouse, laboratory |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | Six founder mice with similar phenotypes noted. One line established. |
Molecular Note | A 7.5 kb genomic fragment derived from a Czech II mammary tumor was used to generate transgenic mice. The fragment contained MMTV envelope and 3' LTR sequences and a truncated portion of the mouse Notch4 gene. |
Hemizygous males are sterile, females can develop tumors as soon as six weeks. Hemizygous females do not lactate. Mating scheme:Tg/+ females to a +/+ males and foster every litter to a mother that lactates. Expected coat color from breeding:Albino
When using the int-3 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002437 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Tg(MMTV-Notch4)3Rnc |
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