These Ncam1 knock-out mice exhibit deficits in spatial learning with reduction in brain weight and olfactory bulb size.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ncam1 | neural cell adhesion molecule 1 |
Mice homozygous for the Ncam1tm1Cgn targeted mutation show a 10% reduction in overall brain weight and 36% reduction in olfactory bulb size. Motor end plates were 14% smaller in NCAM-deficient mice compared to controls, and the formation of junctional folds was delayed. They also show deficits in spatial learning and in the amount of protein-bound alpha-(2,8)-linked polysialic acid. Both homozygous and heterozygous mutant mice are reportedly more aggressive than wildtype controls.
Allele Name | targeted mutation 1, University of Cologne |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | N-CAM-; Ncam-; NCAM-; N-CAM-1- |
Gene Symbol and Name | Ncam1, neural cell adhesion molecule 1 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | A neomycin resistance cassette replaced most of exons 3 and 4 and the intervening intron. Ribonuclease protection assays did not detect wild-type or read-through transcripts or alternative or cryptic splicing in brain tissue from homozygous mutant mice. Western blot analysis of brain tissue using a monoclonal antibody or a polyclonal rabbit serum did not detect protein in homozygous mutant mice. Similarly, immunostaining did not detect protein in mutant olfactory bulb. |
Mutations Made By | Harold Cremer, Developmental Biology Inst. of Marseille |
This strain is difficult to breed homozygously. The suggested breeding scheme is heterozygote x C57BL/6. The expected coat color from breeding is black.
When using the N-CAM KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002405 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Ncam1<tm1Cgn> |
Frozen Mouse Embryo | B6.129P2-Ncam1<tm1Cgn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Ncam1<tm1Cgn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Ncam1<tm1Cgn>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Ncam1<tm1Cgn>/J Frozen Embryo | $3373.50 |
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