These Ncam1 knock-out mice exhibit deficits in spatial learning with reduction in brain weight and olfactory bulb size.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Mice homozygous for the Ncam1tm1Cgn targeted mutation show a 10% reduction in overall brain weight and 36% reduction in olfactory bulb size. Motor end plates were 14% smaller in NCAM-deficient mice compared to controls, and the formation of junctional folds was delayed. They also show deficits in spatial learning and in the amount of protein-bound alpha-(2,8)-linked polysialic acid. Both homozygous and heterozygous mutant mice are reportedly more aggressive than wildtype controls.
|Allele Name||targeted mutation 1, University of Cologne|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||N-CAM-; Ncam-; NCAM-; N-CAM-1-|
|Gene Symbol and Name||Ncam1, neural cell adhesion molecule 1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A neomycin resistance cassette replaced most of exons 3 and 4 and the intervening intron. Ribonuclease protection assays did not detect wild-type or read-through transcripts or alternative or cryptic splicing in brain tissue from homozygous mutant mice. Western blot analysis of brain tissue using a monoclonal antibody or a polyclonal rabbit serum did not detect protein in homozygous mutant mice. Similarly, immunostaining did not detect protein in mutant olfactory bulb.|
|Mutations Made By|| |
Harold Cremer, Developmental Biology Inst. of Marseille
This strain is difficult to breed homozygously. The suggested breeding scheme is heterozygote x C57BL/6. The expected coat color from breeding is black.
When using the N-CAM KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002405 in your Materials and Methods section.