B6.129P2-Ncam1^tm1Cgn/J

Stock number: 002405
Product name: N-CAM KO
Research areas: Cancer Research, Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Neurobiology Research

Description

These Ncam1 knock-out mice exhibit deficits in spatial learning with reduction in brain weight and olfactory bulb size.

Detailed description

Mice homozygous for the Ncam1^tm1Cgn targeted mutation show a 10% reduction in overall brain weight and 36% reduction in olfactory bulb size. Motor end plates were 14% smaller in NCAM-deficient mice compared to controls, and the formation of junctional folds was delayed. They also show deficits in spatial learning and in the amount of protein-bound alpha-(2,8)-linked polysialic acid. Both homozygous and heterozygous mutant mice are reportedly more aggressive than wildtype controls.

Allele

Symbol: Ncam1^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1857222
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ncam1
Marker name: neural cell adhesion molecule 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin resistance cassette replaced most of exons 3 and 4 and the intervening intron. Ribonuclease protection assays did not detect wild-type or read-through transcripts or alternative or cryptic splicing in brain tissue from homozygous mutant mice. Western blot analysis of brain tissue using a monoclonal antibody or a polyclonal rabbit serum did not detect protein in homozygous mutant mice. Similarly, immunostaining did not detect protein in mutant olfactory bulb.