FVB/N-Tg(UcpDta)1Kz/J

Stock number: 002384
Product name: UCP-DTA
Research areas: Diabetes and Obesity Research, Metabolism Research

Description

These UCP-DTA mice express a transgene containing the diphtheria toxin-A-chain (Dta) resulting in a deficiency of brown adipose tissue and an increase of obesity.

Detailed description

Transgenic mice fed a "Western diet" developed marked obesity, insulin resistance, hyperglycemia, and hyperlipidemia. Mice hemizygous for the TgN(UcpDta)1Kz transgene develop early obesity in the absence of hyperphagia indicating an increased metabolic efficiency. Hyperphagia does follow and is present in mice over 7 weeks of age.

It has been the experience of The Jackson Laboratory that hemizygous males have extremely poor reproductive performance and approximately 25% of hemizygous females are successful breeders.

Development

Brown adipose tissue was genetically ablated by incorpation of a toxic transgene, dipththeria toxin A-chain, coupled to regulatory elements of the gene for the brown adipose tissue specific uncoupling protein, UCP.

Allele

Symbol: Tg(UcpDta)1Kz
Name: transgene insertion 1, Leslie P Kozak
MGI accession ID: MGI:2183746
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(UcpDta)1Kz
Marker name: transgene insertion 1, Leslie P Kozak
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: Dta,Diphtheria toxin A chain,
Molecular note: Incorporation of a toxic transgene, diphtheria toxin-A-chain (Dta), coupled to regulatory elements of the gene for brown adipose tissue specific uncoupling protein (Ucp) resulted in the ablation of brown adipose tissue (BAT). Specifically, Dta was inserted into the first exon of Ucp1 at position +120 to create the vector.