This strain is homozygous for the retinal degeneration allele Pde6brd1.
Transgenic mice fed a "Western diet" developed marked obesity, insulin resistance, hyperglycemia, and hyperlipidemia. Mice hemizygous for the TgN(UcpDta)1Kz transgene develop early obesity in the absence of hyperphagia indicating an increased metabolic efficiency. Hyperphagia does follow and is present in mice over 7 weeks of age.
It has been the experience of The Jackson Laboratory that hemizygous males have extremely poor reproductive performance and approximately 25% of hemizygous females are successful breeders.
Brown adipose tissue was genetically ablated by incorpation of a toxic transgene, dipththeria toxin A-chain, coupled to regulatory elements of the gene for the brown adipose tissue specific uncoupling protein, UCP.
|Expressed Gene||Dta, Diphtheria toxin A chain,|
|Site of Expression|
|Allele Name||transgene insertion 1, Leslie P Kozak|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Allele Synonym(s)||BATless; UCP-DTA|
|Gene Symbol and Name||Tg(UcpDta)1Kz, transgene insertion 1, Leslie P Kozak|
|Promoter||Ucp, mitochondrial uncoupling protein,|
|Expressed Gene||Dta, Diphtheria toxin A chain,|
|Strain of Origin||FVB/N|
|Molecular Note||Incorporation of a toxic transgene, diphtheria toxin-A-chain (Dta), coupled to regulatory elements of the gene for brown adipose tissue specific uncoupling protein (Ucp) resulted in the ablation of brown adipose tissue (BAT). Specifically, Dta was inserted into the first exon of Ucp1 at position +120 to create the vector.|
Transgenic mice have micropthalamia and cataracts related to integration site and can be identified at the age of 16 days.
It has been the experience of The Jackson Laboratory that hemizygous males have extremely poor reproductive performance and approximately 25% of hemizygous females are successful breeders. Recommended breeding scheme is hemizygous female x non-carrier or FVB/NJ (stock 1800) male. Expected coat color from breeding:Albino
When using the UCP-DTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #002384 in your Materials and Methods section.
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