NOR.NOD-(Il1-Ada)/LtJ

Stock number: 002347
Product name: NOR.NOD.IL1
Strain synonyms: NOR.NOD-Chr2
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Detailed description

This NOR/Lt congenic strain is carrying alleles derived from strain NOD/Lt for interleukin 1a (Il1a^NOD), adenosine deaminase (Ada) and insulin dependent diabetes susceptibility 13 (Idd13^NOD) loci. Neither males nor females from this congenic strain when aged to 40 weeks showed diabetes development. This strain is useful for dissecting the role of non-MHC susceptibility genes in autoimmune diabetes.

Development

The congenic stock NOR.NOD-(Il1-Ada)/Lt, carrying a defined segment of Chr 2 that includes the NOD/Lt insulin dependent diabetes susceptibility locus Idd13 (71cM), was produced by mating mice from NOR/Lt to NOD/Lt followed by crossing 20 times to NOR/Lt with selection of NOD Il1 (94cM) genotype at each generation. An additional genome wide scan verified NOR homozygosity at all other chromosomal regions known to differ between NOD/Lt and NOR/Lt. This congenic strain retains NOR/Lt genome from D2Mit277 (69.0cM) through D2Mit395 (66.9cM) . The Il1-Ada region of Chr 2 in NOR/Lt was originally derived from the C57BL/6 contribution to strain C57BLKS/J; thus, this congenic strain retains B6 alleles for the microsatellite markers D2Mit277 through D2Mit395, including the functional gene B2m (69.0cM) (Reifsnyder et al, 2005). In 2006, the T1DR received this strain at N21F28.

Allele

Symbol: Idd13^NOD/ShiLt
Name: NOD/ShiLt
MGI accession ID: MGI:3036815
Generation method: QTL
Marker symbol: Idd13
Marker name: insulin dependent diabetes susceptibility 13
Chromosome: 2
Strain of origin: NOD/ShiLt
Molecular note: This allele confers defective cytokine response (IL-2, IL-4, IFN-gamma, CSF-2) when stimulated with antigens compared to C57BL/6.
General note: B6.NOD-Idd13 congenic animals exhibit defective iNKT cell induced IFN-gamma response. This phenotype resembles that of the NOD parental.

Allele

MGI accession ID: MGI:96536
Marker symbol: Il1
Marker name: interleukin 1 complex
Chromosome: 2

Allele

MGI accession ID: MGI:87916
Marker symbol: Ada
Marker name: adenosine deaminase
Chromosome: 2