These Plau knock-out mice exhibit occasional fibrin deposition and excessive fibrin deposition when combined with the Plat knock-out. They are suitable for use in applications related to the study of the fibrinolytic system.
Dr. Peter Carmeliet, University of Leuven
Homozygotes develop normally, are fertile and have a normal life span. Rectal prolapse of a non-infectious origin develops in 9% of homozygotes and/or extensive non-healing ulcerations occur at the eyelids and around the face. Small, focal fibrin deposits are occasionally seen in the intestines and in the sinusoids of the liver, and excessive fibrin deposits are seen in ulcerated skin or prolapsed rectum. Pulmonary clot lysis is comparable to that seen in normal wildtype siblings. Endotoxin induced venous thrombosis is increased over normal wildtype siblings. Fibrin dissolution by PLAU-deficient macrophages is greatly reduced but macrophage invasion into the peritoneal cavity after thioglycollate injection is unaffected. Homozygous knockout mice have increased levels of Abeta42 and Abeta40 in plasma. Brain Abeta levels are not significantly different than controls. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
|Allele Name||targeted mutation 1, Richard C Mulligan|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Plautm1Mlg; targeted mutation 1, Richard C Mulligan|
|Gene Symbol and Name||Plau, plasminogen activator, urokinase|
|Gene Synonym(s)||BDPLT5; ATF; URK; urokinase-type plasminogen activator; UPA; UPAM; u-PA; QPD; uPA|
|Strain of Origin||129S2/SvPas|
|Molecular Note||The gene was disrupted using neomycin resistance cassette. The vector replaced genomic sequences encompassing all but 23 amino acids of the coding sequence. Targeting was confirmed by the absence of gene specific mRNA and immunoreactivity.|
|Mutations Made By|| |
Dr. Peter Carmeliet, University of Leuven
This strain is maintained by homozygous sibling matings.
When using the u-PA- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002328 in your Materials and Methods section.
|Heterozygous for Plau<tm1Mlg>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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