These Serpine1 knock-out mice exhibit an increase of pulmonary clot lysis. They are suitable for use in applications related to the study of the fibrinolytic system.
Dr. Peter Carmeliet, University of Leuven
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Serpine1 | serine (or cysteine) peptidase inhibitor, clade E, member 1 |
Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.
Allele Name | targeted mutation 1, Richard C Mulligan |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | PAI-1-; Planh1tm1; Serpine1tm1Mg |
Gene Symbol and Name | Serpine1, serine (or cysteine) peptidase inhibitor, clade E, member 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 5 |
Molecular Note | A PGK-neomycin resistance cassette replaced all of the Serpine1 coding sequence and part of the promoter region including the transcription initiation site. Northern blot analysis did not detect Serpine1 mRNA in endotoxin-treated homozygous mutant liver. Neither untreated nor endotoxin-treated homozygous mutant kidney produced detectable SERPINE1 immunoreactivity. A monoclonal antibody based immunofunctional assay did not detect protein levels in plasma of endotoxin-treated homozygous mutant mice. |
Mutations Made By | Dr. Peter Carmeliet, University of Leuven |
When using the PAI-1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002324 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Serpine1<tm1Mlg> |
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