FVB.129S2-Serpine1^tm1Mlg/J

Stock number: 002324
Product name: PAI-1^-
Research areas: Cancer Research, Internal/Organ Research

Description

These Serpine1 knock-out mice exhibit an increase of pulmonary clot lysis. They are suitable for use in applications related to the study of the fibrinolytic system.

Detailed description

Mice homozygous for this mutation develop normally and are viable and fertile. Compared to wild type mice, pulmonary clot lysis is increased in the heterozygote and further increased in the homozygote. Endotoxin induced venous thrombosis is decreased compared to wild type mice. Thus, disruption of the Serpine1 gene induces a mild hyperfibrinolytic state. Hemostasis is normal in homozygous mutants.

Allele

Symbol: Serpine1^tm1Mlg
Name: targeted mutation 1, Richard C Mulligan
MGI accession ID: MGI:1857287
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: PAI-1^-; Planh1^tm1; Serpine1^tm1Mg
Marker symbol: Serpine1
Marker name: serine (or cysteine) peptidase inhibitor, clade E, member 1
Marker synonyms: PAI; Pai1; PAI-1; PAI1A; Pai1aa; Planh; PLANH1; RATPAI1A
Chromosome: 5
Strain of origin: 129S2/SvPas
Molecular note: A PGK-neomycin resistance cassette replaced all of the Serpine1 coding sequence and part of the promoter region including the transcription initiation site. Northern blot analysis did not detect Serpine1 mRNA in endotoxin-treated homozygous mutant liver. Neither untreated nor endotoxin-treated homozygous mutant kidney produced detectable SERPINE1 immunoreactivity. A monoclonal antibody based immunofunctional assay did not detect protein levels in plasma of endotoxin-treated homozygous mutant mice.