Female Inhbb knock-out mice exhibit impaired reproductive ability and all mice exhibit eye lesions. They are suitable for use in applications related to the study of mesoderm formation and patterning.
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Inhbb | inhibin beta-B |
Homozygous mice are deficient in activin B (betaB:betaB), activin AB (betaA:betaB) and inhibin B (alpha:betaB). Heterozygotes appear normal. Homozygous mutants complete embryonic development and are viable. They are born, however, with open eyes which leads to the development of eye lesions. Homozygous males breed normally but mutant females exhibit a profoundly impaired reproductive ability, characterized by perinatal lethality of their offspring. Mutant females have an increased gestation time and decreased nursing ability that may be the result of impaired milk let-down. There is an upregulation of beta-A activin which may contribute to the phenotype.
Allele Name | targeted mutation 1, Rudolf Jaenisch |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | bb1; bb2; Inhbb- |
Gene Symbol and Name | Inhbb, inhibin beta-B |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 1 |
Molecular Note | A neomycin resistance cassette replaced a 1.3 kb region of the gene, which spanned the translational start codon, the signal peptide coding region, and most of exon 1 of the gene. In the construct designated bb1, the neomycin cassette was inserted in the same transcriptional orientation as the gene; in the construct designated bb2, the neomycin cassette was inserted in the reverse orientation. |
Mutations Made By | Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology |
When maintaining a live colony, homozygous males may be bred to wildtype female siblings.
Homozygous females exhibit a profoundly impaired reproductive ability, characterized by perinatal lethality of their offspring.
When using the βB-1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002323 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Inhbb<tm1Jae> |
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