129S4/SvJae-Inhbb^tm1Jae/J

Stock number: 002323
Product name: βB-1 KO
Research areas: Cancer Research, Developmental Biology Research, Reproductive Biology Research, Sensorineural Research

Description

Female Inhbb knock-out mice exhibit impaired reproductive ability and all mice exhibit eye lesions. They are suitable for use in applications related to the study of mesoderm formation and patterning.

Detailed description

Homozygous mice are deficient in activin B (betaB:betaB), activin AB (betaA:betaB) and inhibin B (alpha:betaB). Heterozygotes appear normal. Homozygous mutants complete embryonic development and are viable. They are born, however, with open eyes which leads to the development of eye lesions. Homozygous males breed normally but mutant females exhibit a profoundly impaired reproductive ability, characterized by perinatal lethality of their offspring. Mutant females have an increased gestation time and decreased nursing ability that may be the result of impaired milk let-down. There is an upregulation of beta-A activin which may contribute to the phenotype.

Allele

Symbol: Inhbb^tm1Jae
Name: targeted mutation 1, Rudolf Jaenisch
MGI accession ID: MGI:1857203
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Inhbb
Marker name: inhibin beta-B
Chromosome: 1
Strain of origin: 129S4/SvJae
Molecular note: A neomycin resistance cassette replaced a 1.3 kb region of the gene, which spanned the translational start codon, the signal peptide coding region, and most of exon 1 of the gene. In the construct designated bb1, the neomycin cassette was inserted in the same transcriptional orientation as the gene; in the construct designated bb2, the neomycin cassette was inserted in the reverse orientation.