These B2m knock-out mice exhibit little if any MHC class I protein expression on the cell surface with deficiency in CD8+ cytotoxic T-cells, NK1+ T cells, and decreased levels of serum Ig.
Dr. Derry Roopenian, The Jackson Laboratory
Mice homozygous for the B2mtm1Unc targeted mutation have little if any MHC class I protein expression on the cell surface. There are few CD8+ cytotoxic T-cells and under some circumstances a compensatory increase in CD4+ cytotoxic T-cells. Immune responses involving CD8+ T-cells are severely deficient providing a model to assess the role of CD8+ cells and class I MHC in various experimental systems. Other traits of B2M deficient mice include: NK cell deficiency, NK1+ T cells deficiency and decreased levels of serum Ig. Some forms of lupus autoimmunity are severely reduced. (Christianson, J Immunol 156:4932-9, 1996). Hemachromatosis has been noted in certain genetic backgrounds (Rothenberg BE, Voland JR, Proc Natl Acad Sci USA 93:1529-34, 1996). The B2mtm1Un mutation backcrossed to the NOD/Lt strain serves as a MHC class I-negative, CD8+ deficient diabetes resistant stock for comparison to the NOD/LtJ strain (Stock No. 001976). The elimination of cell surface MHC class I expression blocks both insulitis and autoimmune diabetes in NOD/Lt mice. A disadvantage of this strain is that CD4+ T cells are not tolerant to MHC class I positive syngeneic cells. These mice provide an excellent source for insulitis-free, MHC class I-bare NOD islets for transplantation studies.
The B2mtm1Unc mutant strain was developed in the laboratory of Dr. Beverly Koller and Dr. Oliver Smithies at the University of North Carolina at Chapel Hill. It was generated by a targeted disruption of the B2m gene. The 129-derived E14TG2a ES cell line was used. The NOD/LtJ strain was produced in the laboratory of Dr. Derry Roopenian at The Jackson Laboratory by backcrossing the B2mtm1Unc mutation 10 times to NOD/LtJ inbred mice.
|Allele Name||targeted mutation 1, University of North Carolina|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||I0; MHC-I-; b2mnull; beta2-m-KO; beta2M-; beta2MKO; beta2m0; beta2mnull; beta2mo; beta2mtm1Unc|
|Gene Symbol and Name||B2m, beta-2 microglobulin|
|Gene Synonym(s)||beta 2 microglobulin; beta2-m; Ly-m11; lymphocyte antigen m11; Ly-m11; IMD43|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Insertion of a neomycin-resistance gene into the second exon.|
|Mutations Made By|| |
Dr. Oliver Smithies, University of North Carolina at Chapel Hill
This B2mtm1Unc strain is maintained by mating heterozygous and homozygous mice. These mice are immunodeficient and need to be maintained in a high barrier environment with sterile food and water. Expected coat color from breeding is Albino.
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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