129S2/SvPas-Smarcad1^{Gt(6LSN)6028Gos}/J

Stock number: 002303
Product name: Etl-1 line 6028
Research areas: Cell Biology Research, Developmental Biology Research, Research Tools

Description

These mice harbor the Smarcad1^{Gt(6LSN)6028Gos} gene trap allele showing lacZ expression particularly in the central nervous system and epithelial cells.

Detailed description

The Smarcad1 gene (formerly Etl-1) was identified in close proximity to a lacZ enhancer trap integration in the mouse genome showing a specific beta-galactosidase staining pattern during development. In situ analysis revealed a widespread but not ubiquitous expression throughout development with particularly high levels in the central nervous system and epithelial cells. The amino acid sequence of the Smarcad1 protein shows strong similarity to the Drosophila brahma protein involved in the regulation of homeotic genes and to the yeast transcriptional activator protein SNF2/SWI2 as well as to the RAD54 protein and the helicase-related yeast proteins STH1 and MOT1. Smarcad1 is implicated in gene regulation and/or influencing of chromatin structure, and may be involved in gene regulating pathways during mouse development.

Development

The 6LSN construct is one of several enhancer trap constructs designed with the lacZ reporter gene downstream of a TATA box and translation initiation codon with an attached neomycin resistance cassette. 6LSN was electroporated into 129S2/SvPas derived D3 ES cells. One of the resulting cell lines, 6028, was injected into CD1 blastocysts and gave rise to the 129S2/SvPas-Smarcad1^{Gt(6LSN)6028Gos} mouse strain. In 1994 homozygous siblings from this strain were mated to generate embryos for cryopreservation at The Jackson Laboratory. (Gossler et al., 1989; Soininen et al., 1992.)

Allele

Symbol: Smarcad1^{Gt(6LSN)6028Gos}
Name: gene trap 6028, Achim Gossler
MGI accession ID: MGI:2177962
Generation method: Gene trapped
Attributes: Reporter; Null/Knockout
Marker symbol: Smarcad1
Marker name: SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1
Chromosome: 6
Strain of origin: 129S2/SvPas
Molecular note: Insertion of the vector 6LSN into the gene. A beta-galactosidase fusion protein is expressed from this allele.