The Smarcad1 gene (formerly Etl-1) was identified in close proximity to a lacZ enhancer trap integration in the mouse genome showing a specific beta-galactosidase staining pattern during development. In situ analysis revealed a widespread but not ubiquitous expression throughout development with particularly high levels in the central nervous system and epithelial cells. The amino acid sequence of the Smarcad1 protein shows strong similarity to the Drosophila brahma protein involved in the regulation of homeotic genes and to the yeast transcriptional activator protein SNF2/SWI2 as well as to the RAD54 protein and the helicase-related yeast proteins STH1 and MOT1. Smarcad1 is implicated in gene regulation and/or influencing of chromatin structure, and may be involved in gene regulating pathways during mouse development.
The 6LSN construct is one of several enhancer trap constructs designed with the lacZ reporter gene downstream of a TATA box and translation initiation codon with an attached neomycin resistance cassette. 6LSN was electroporated into 129S2/SvPas derived D3 ES cells. One of the resulting cell lines, 6028, was injected into CD1 blastocysts and gave rise to the 129S2/SvPas-Smarcad1Gt(6LSN)6028Gos mouse strain. In 1994 homozygous siblings from this strain were mated to generate embryos for cryopreservation at The Jackson Laboratory. (Gossler et al., 1989; Soininen et al., 1992.)
|Allele Name||gene trap 6028, Achim Gossler|
|Allele Type||Gene trapped (Reporter, Null/Knockout)|
|Gene Symbol and Name||Smarcad1, SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Insertion of the vector 6LSN into the gene. A beta-galactosidase fusion protein is expressed from this allele.|