These rederived G93A-SOD1 transgenic mice carry a lower copy number than the original transgenic strain and may be useful in studying neuromuscular disorders, including Amyotrophic Lateral Sclerosis (ALS or Lou Gehrig's Disease).
Dr. Mark E. Gurney, Tetra Discovery Partners
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice carrying the transgene exhibit paralysis resulting in a reduced life expectancy. They serve as a model for human amyotrophic lateral sclerosis (ALS). The transgene carries a variant of the human Superoxide dismutase-1 gene (glycine to alanine at position 93). Paralysis is due to loss of motor neurons from the spinal cord. The onset of the ALS phenotype is delayed compared to the original high copy number strain (SOD1*G93A)1Gur, Stock No. 002726) because of a reduction in transgenic copy number. This reduction most likely occurred during the importation and establishment of the original high copy number mutant transgenic strain. Mice become paralyzed in one or more limbs beginning around six to seven months of age. Life expectancy is normally four to six weeks beyond onset of symptoms.
This transgenic was made in the laboratory of Dr. Mark E. Gurney at Northwestern University. The transgene is the mutant human SOD1 containing the Gly93 -->Ala (G93A) substitution.
Expressed Gene | SOD1, superoxide dismutase 1, human |
---|---|
Site of Expression |
Allele Name | transgene insertion dl 1, Mark E Gurney |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | G1L; G93A SOD1; mSOD1-Tg; Tg(SOD1-G93A)dl1Gur; TgN(SOD1-G93A)1Gurdl; TgN(SOD1-G93A)1Gurdl; TgN[SOD1-G93A]dl1Gur; TgNSOD1-G93A |
Gene Symbol and Name | Tg(SOD1*G93A)dl1Gur, transgene insertion dl 1, Mark E Gurney |
Gene Synonym(s) | |
Promoter | SOD1, superoxide dismutase 1, human |
Expressed Gene | SOD1, superoxide dismutase 1, human |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | 12 |
General Note | Transgenic mice on a C57BL/6 congenic background exhibit paralysis resulting in a reduced life expectancy, and may serve as a model for human amyotrophic lateral sclerosis (ALS). Paralysis in these mice is due to loss of motor neurons from the spinal cord. The onset of the ALS phenotype in these mice is delayed compared to mice carrying the original high copy number transgene Tg(SOD1*G93A)1Gur. Transgenic mice on a genetic background involving C57BL/6 and SJL become paralyzed in one or more limbs beginning around six to seven months of age. Life expectancy for these mice is normally four to six weeks beyond onset of symptoms. |
Molecular Note | This subline has approximately 30% fewer copies of the transgene construct than the high copy number line, Tg(SOD1*G93A)1Gur. The reduction most likely occurred during the importation and establishment of the high copy number mutant transgenic line. The transgene is composed of a variant of the human superoxide dismutase 1 gene (SOD1) carrying a glycine to alanine substitution at position 93 (G93A). The G93A mutation does not alter the activity of the protein. |
Mutations Made By | Dr. Mark Gurney, Tetra Discovery Partners |
This strain is maintained by mating hemizygous carriers (preferably males) to B6SJLF1 hybrids. We have established an 8 month lifetime maximum for this strain that is compatible with The Jackson Laboratory policy for humane husbandry (i.e. paralysis has affected the mouse to the extent that it can not eat, drink, or move freely). Expected coat color from breeding;Black, White Bellied Agouti, Albino, Light Gray and Tan w/ pink eyes
When using the G93A SOD1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002300 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(SOD1*G93A)<dl>1Gur |
Frozen Mouse Embryo | B6SJL-Tg(SOD1*G93A)<dl>1Gur/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6SJL-Tg(SOD1*G93A)<dl>1Gur/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6SJL-Tg(SOD1*G93A)<dl>1Gur/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6SJL-Tg(SOD1*G93A)<dl>1Gur/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.