Mice homozygous for the Ntf3tm1Jae targeted mutation are smaller than their normal littermates and most die shortly after birth. Survivors die prior to weaning. They display limb ataxia, an inability to position the limbs properly when attempting to move, and there is a tendency for all four limbs to intermittently stiffen in an extensor posture. Autopsy showed all peripheral ganglia markedly smaller in the mutant. Spinal proprioceptive afferents and their peripheral sense organs are completely absent. Heterozygous mice appear normal; however, the number of muscle spindles in heterozygotes is half that of normal wildtype siblings. There is approximately a 50% reduction in the sympathetic superior cervical ganglion (SCG) neurons caused by excessive apoptosis of sympathetic neuroblasts during neurogenesis.
|Allele Name||targeted mutation 1, Rudolf Jaenisch|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||NT-3-; NT3-|
|Gene Symbol and Name||Ntf3, neurotrophin 3|
|Strain of Origin||129S4/SvJae|
|Molecular Note||The entire coding region for the Ntf3 gene was replaced by a neomycin cassette.|
|Mutations Made By|| |
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation die shortly after birth.
When using the NT-3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002275 in your Materials and Methods section.