B6.129S-Fn1^tm1Hyn/J

Stock number: 002270
Product name: FN.null
Research areas: Cardiovascular Research, Developmental Biology Research

Description

These fibronectin 1 ( Fn1) knock-out mice exhibit embryonic lethality with developmental defects appearing after day 8. They are suitable for use in applications related to the study of roles for fibronectin during early development.

Detailed description

Mice homozygous for the Fn1^tm1Hyn targeted mutation die during early embryonic development. Blastocyst development and implantation of homozygotes is normal. Gastrulation is initiated and appears normal, including extensive mesodermal movement. From embryonic day 8 onwards homozygous mutant embryos deteriorate through the 10th and 11th days of gestation. Homozygous mutant embryos have a shortened anterior-posterior axes, fail to develop a notochord or somites, and have abnormal development of the heart, blood vessels, and yolk sac indicating a general deficiency in mesodermally derived tissues. Heterozygous mice are viable for at least 2 years and appear healthy and approximately the same size as wild-type littermates. Plasma levels of fibronectin in heterozygotes are 50% lower than normal wildtype siblings.

Development

A PGK-neomycin resistance cassette replaced 0.8 kb of the gene including the translation initiation site and part of the signaling sequence. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells. The mice were bred to 129S4/SvJae then backcrossed to C57BL/6 for at least 11 generations.

Allele

Symbol: Fn1^tm1Hyn
Name: targeted mutation 1, Richard Hynes
MGI accession ID: MGI:1857170
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Fn1
Marker name: fibronectin 1
Chromosome: 1
Strain of origin: 129S2/SvPas
Molecular note: A PGK-neomycin resistance cassette replaced 0.8 kb of the gene including the translation initiation site and part of the signaling sequence. Plasma concentrations of fibronectin in heterozygotes were one-half those of wild-type littermates. The encoded protein was not detectable in immunoprecipitations from cultures of homozygous mutant E7.5 embryos.