Overview

Also Known As: FN.null

These fibronectin 1 ( Fn1) knock-out mice exhibit embryonic lethality with developmental defects appearing after day 8. They are suitable for use in applications related to the study of roles for fibronectin during early development.

Donating Investigator

Dr. Richard Hynes, Massachusetts Institute of Technology

Genetic overview

Genetic Background	Generation
000664 C57BL/6J

Fn1^tm1Hyn

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Fn1	fibronectin 1

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Research Applications

Cardiovascular Research
Developmental Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for the Fn1^tm1Hyn targeted mutation die during early embryonic development. Blastocyst development and implantation of homozygotes is normal. Gastrulation is initiated and appears normal, including extensive mesodermal movement. From embryonic day 8 onwards homozygous mutant embryos deteriorate through the 10th and 11th days of gestation. Homozygous mutant embryos have a shortened anterior-posterior axes, fail to develop a notochord or somites, and have abnormal development of the heart, blood vessels, and yolk sac indicating a general deficiency in mesodermally derived tissues. Heterozygous mice are viable for at least 2 years and appear healthy and approximately the same size as wild-type littermates. Plasma levels of fibronectin in heterozygotes are 50% lower than normal wildtype siblings.

Development

A PGK-neomycin resistance cassette replaced 0.8 kb of the gene including the translation initiation site and part of the signaling sequence. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells. The mice were bred to 129S4/SvJae then backcrossed to C57BL/6 for at least 11 generations.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

George EL; Georges-Labouesse EN; Patel-King RS; Rayburn H; Hynes RO. 1993. Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin. Development 119(4):1079-91PubMed: 8306876 MGI: J:16247

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Genetics

Fn1^tm1Hyn

Allele Symbol: Fn1^tm1Hyn

Allele Name	targeted mutation 1, Richard Hynes
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Fn1^tm1Hyn; targeted mutation 1, Richard Hynes
Gene Symbol and Name	Fn1, fibronectin 1
Gene Synonym(s)	GFND2; FINC; ED-B; FN; Fn-1; FNZ; MSF; Fn-1; LETS; CIG; GFND; FIBNEC; fn-1; Fn; SMDCF
Strain of Origin	129S2/SvPas
Chromosome	1
Molecular Note	A PGK-neomycin resistance cassette replaced 0.8 kb of the gene including the translation initiation site and part of the signaling sequence. Plasma concentrations of fibronectin in heterozygotes were one-half those of wild-type littermates. The encoded protein was not detectable in immunoprecipitations from cultures of homozygous mutant E7.5 embryos.
Mutations Made By	Dr. Richard Hynes, Massachusetts Institute of Technology

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Fn1^tm1Hyn related

Cardiovascular Research
- Heart Abnormalities
- Vascular Defects
Developmental Biology Research
- Defects in Extracellular Matrix Molecules
- Internal/Organ Defects
  - heart: vasculature
  - heart

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Fn1^tm1Hyn/Fn1^tm1Hyn
involves: 129S2/SvPas * C57BL/6J

cardiovascular system phenotype

abnormal cardiac jelly morphology
- at E8.5, cardiac jelly is deficient

abnormal dorsal aorta morphology
- at E8.5, severely affected embryos show absence of dorsal aortae while less severely affected embryos exhibit distended dorsal aortae containing only a few blood cells

abnormal endocardium morphology
- at E8.5, the mutant endocardium is either indistinguishable from the thickened myocardium or absent

abnormal heart development
- at E8.5, 13 of 20 mutant embryos have a visible primitive heart whereas the remaining seven do not
- in severely affected embryos, heart primordia fail to fuse and are positioned laterally

abnormal vasculogenesis
- at E8.5, homozygotes display defective vasculogenesis in the yolk sac
- mutant embryonic vessels appear variable and deformed while extraembryonic vasculature is also defective

absent vitelline blood vessels
- at E8.5, blood vessels are detected in the exocoelomic cavity rather than in the yolk sac

thick myocardium
- at E8.5, homozygotes display a thickened myocardium

embryo phenotype

abnormal amnion morphology
- at E7.5, mutant embryos possess three germ layers and normal extraembryonic membranes except for a concave amnion
- at E8.0, the mutant amnion is undersized and closely apposed to the embryo, displaying a pressure deficit in the amniotic cavity

abnormal developmental patterning

abnormal embryonic neuroepithelium morphology
- at E8.0, homozygotes display multiple bends and distortions in the neural ectoderm

abnormal embryonic tissue morphology

abnormal extraembryonic tissue morphology

abnormal lateral plate mesoderm morphology
- by E8.5, lateral mesoderm flanking the neural tube is reduced

abnormal mesoderm development
- at E8.0, homozygotes exhibit a deficit in the trunk and headfold mesoderm
- by E8.5, mutant headfolds appear small and misshapen, with a deficit in underlying mesoderm and many pyknotic cells

abnormal neural tube morphology

abnormal visceral yolk sac morphology
- at E8.5, the mesodermal and endodermal layers of the mutant yolk sac appear to split apart

absent notochord
- at E8.5, homozygotes lack an organized notochord; instead, the endodermal lining of the future midgut is juxtaposed to the neural tube

absent somites
- at E8.5, mutant embryos lack somites whereas wild-type embryos contain 8 to 12 pairs of somites; however, condensations of cells suggestive of incipient somites are detected in 3 of 20 mutants

absent visceral yolk sac blood islands

absent vitelline blood vessels
- at E8.5, blood vessels are detected in the exocoelomic cavity rather than in the yolk sac

decreased embryo size
- at E7.5, mutant embryos appear relatively normal, though slightly smaller than wild-type embryos

embryonic growth retardation
- starting at E8.0, homozygotes appear developmentally retarded and abnormal

failure of chorioallantoic fusion
- at E8.5, the allantois has yet not fused with the chorion

failure of initiation of embryo turning
- at E8.5, none of the mutant embryos have initiated turning

kinked neural tube
- at E8.5, homozygotes display a kinked neural tube

short rostral-caudal axis
- at E8.0, homozygotes display a shortened anterior-posterior axis

growth/size/body region phenotype

decreased embryo size
- at E7.5, mutant embryos appear relatively normal, though slightly smaller than wild-type embryos

embryonic growth retardation
- starting at E8.0, homozygotes appear developmentally retarded and abnormal

mortality/aging

embryonic lethality during organogenesis, complete penetrance
- homozygotes develop severe embryonic abnormalities from E8.0 onwards and undergo degeneration during E10 and E11

muscle phenotype

thick myocardium
- at E8.5, homozygotes display a thickened myocardium

nervous system phenotype

abnormal embryonic neuroepithelium morphology
- at E8.0, homozygotes display multiple bends and distortions in the neural ectoderm

abnormal neural tube morphology

kinked neural tube
- at E8.5, homozygotes display a kinked neural tube

References

George EL; Georges-Labouesse EN; Patel-King RS; Rayburn H; Hynes RO. 1993. Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin. Development 119(4):1079-91PubMed: 8306876 MGI: J:16247

Additional References

Georges-Labouesse EN; George EL; Rayburn H; Hynes RO. 1996. Mesodermal development in mouse embryos mutant for fibronectin. Dev Dyn 207(2):145-56PubMed: 8906418 MGI: J:36210
George EL; Baldwin HS; Hynes RO. 1997. Fibronectins are essential for heart and blood vessel morphogenesis but are dispensable for initial specification of precursor cells. Blood 90(8):3073-81PubMed: 9376588 MGI: J:43434

Additional - Fn1^tm1Hyn related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Fn1^tm1Hyn
- Genotyping resources and troubleshooting
Breeding Considerations

Heterozygotes are viable and fertile. Homozygotes die during early embryonic development.
- Additional Breeding and Husbandry Support
Citation
When using the FN.null mouse strain in a publication, please cite the originating article(s) and include JAX stock #002270 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Fn1<tm1Hyn>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: FN.null

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Fn1tm1Hyn

Allele Symbol: Fn1tm1Hyn

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Fn1tm1Hyn related

Mammalian Phenotype Terms by Genotype

Genotype: Fn1tm1Hyn/Fn1tm1Hyninvolves: 129S2/SvPas * C57BL/6J

cardiovascular system phenotype

embryo phenotype

growth/size/body region phenotype

mortality/aging

muscle phenotype

nervous system phenotype

References

Additional References

Additional - Fn1tm1Hyn related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Fn1^tm1Hyn

Allele Symbol: Fn1^tm1Hyn

Genotype: Fn1^tm1Hyn related

Genotype: Fn1^tm1Hyn/Fn1^tm1Hyn
involves: 129S2/SvPas * C57BL/6J

Additional - Fn1^tm1Hyn related