These fibronectin 1 ( Fn1) knock-out mice exhibit embryonic lethality with developmental defects appearing after day 8. They are suitable for use in applications related to the study of roles for fibronectin during early development.
Dr. Richard Hynes, Massachusetts Institute of Technology
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fn1 | fibronectin 1 |
Mice homozygous for the Fn1tm1Hyn targeted mutation die during early embryonic development. Blastocyst development and implantation of homozygotes is normal. Gastrulation is initiated and appears normal, including extensive mesodermal movement. From embryonic day 8 onwards homozygous mutant embryos deteriorate through the 10th and 11th days of gestation. Homozygous mutant embryos have a shortened anterior-posterior axes, fail to develop a notochord or somites, and have abnormal development of the heart, blood vessels, and yolk sac indicating a general deficiency in mesodermally derived tissues. Heterozygous mice are viable for at least 2 years and appear healthy and approximately the same size as wild-type littermates. Plasma levels of fibronectin in heterozygotes are 50% lower than normal wildtype siblings.
A PGK-neomycin resistance cassette replaced 0.8 kb of the gene including the translation initiation site and part of the signaling sequence. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells. The mice were bred to 129S4/SvJae then backcrossed to C57BL/6 for at least 11 generations.
Allele Name | targeted mutation 1, Richard Hynes |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | FN.null; Fn-; FNnull |
Gene Symbol and Name | Fn1, fibronectin 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | A PGK-neomycin resistance cassette replaced 0.8 kb of the gene including the translation initiation site and part of the signaling sequence. Plasma concentrations of fibronectin in heterozygotes were one-half those of wild-type littermates. The encoded protein was not detectable in immunoprecipitations from cultures of homozygous mutant E7.5 embryos. |
Mutations Made By | Dr. Richard Hynes, Massachusetts Institute of Technology |
Heterozygotes are viable and fertile. Homozygotes die during early embryonic development.
When using the FN.null mouse strain in a publication, please cite the originating article(s) and include JAX stock #002270 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Fn1<tm1Hyn> |
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