These Bdnf knock-out mice exhibit severe deficiencies in coordination and balance, associated with excessive degeneration in several sensory ganglia including the vestibular ganglion.
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Bdnf | brain derived neurotrophic factor |
Mice heterozygous for the Bdnftm1Jae mutation show about 1/2 normal levels of Bdnf mRNA, but appear normal. Mice homozygous for the Bdnftm1Jae mutation are smaller than normal siblings and most die within the second postnatal week. They have defective coordination of movements and balance. They also exhibit head bobbing and spinning during periods of hyperactivity. There are rare survivors to adulthood. There is excessive degeneration in all sensory ganglia examined, including the vestibular ganglion. Motor neurons are not affected. BDNF can prevent death of central motor neurons and other neurons in vitro, but does not affect these in vivo.
The Bdnftm1Jae mutant strain was developed in the laboratory of Dr. Rudolf Jaenisch at the Whitehead Institute for Biomedical Research at the Massachusetts Institute of Technology. A part of exon 5 of the gene was deleted and replaced by a neomycin cassette. This deletion left 49 and 30 amino acids in the N and C termini, respectively. The 129-derived J1 ES cell line was used.
Allele Name | targeted mutation 1, Rudolf Jaenisch |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | BDNF-; BDNF-KO |
Gene Symbol and Name | Bdnf, brain derived neurotrophic factor |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 2 |
Molecular Note | A part of exon 5 of the gene was deleted and replaced by a neomycin cassette. This deletion left 49 and 30 amino acids in the N and C termini, respectively. Northern blot analysis on RNA derived from cerebral cortex showed that no detectable transcript is expressed from this allele. |
Mutations Made By | Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology |
The Bdnftm1Jae strain is maintained by mating heterozygous mice to normal wildtype siblings. Heterozygous mice and normal wildtype siblings may be ordered. Expected coat colors from breeding:Black, White Bellied Agouti, Albino
When using the BDNF- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002267 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Bdnf<tm1Jae> |
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