Mice homozygous for the Bdnf knock-out show degeneration in sensory ganglia, and may be useful in studies of neurotrophic factor defects, hearing defects, and nociception.
IMR Colony, The Jackson Laboratory
Mice heterozygous for the Bdnftm1Jae mutation show about half normal levels of Bdnf mRNA, but appear normal. Mice homozygous for the Bdnftm1Jae mutation are smaller than normal siblings and most die within the second postnatal week. They have defective coordination of movements and balance. They also exhibit head bobbing and spinning during periods of hyperactivity. There are rare survivors to adulthood. There is excessive degeneration in all sensory ganglia examined, including the vestibular ganglion. Motor neurons are not affected. BDNF can prevent death of central motor neurons and other neurons in vitro, but does not affect these in vivo.
|Allele Name||targeted mutation 1, Rudolf Jaenisch|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||BDNF-; BDNF-KO|
|Gene Symbol and Name||Bdnf, brain derived neurotrophic factor|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A part of exon 5 of the gene was deleted and replaced by a neomycin cassette. This deletion left 49 and 30 amino acids in the N and C termini, respectively. Northern blot analysis on RNA derived from cerebral cortex showed that no detectable transcript is expressed from this allele.|
|Mutations Made By|| |
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
The Bdnftm1Jae strain is maintained by mating heterozygous mice to normal wildtype siblings. Heterozygous x wildtype breeder pairs are supplied. Expected coat color from breeding:Black
When using the BDNF- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002266 in your Materials and Methods section.