These Bcl2 knock-out mice exhibit growth retardation and renal failure as a result of polycystic kidney disease.
Dr. Stanley J. Korsmeyer, Dana-Farber Cancer Institute
Mice homozygous for the Bcl2tm1Sjk targeted mutation appear normal at birth; however, growth retardation is evident by one week of age with considerable heterogeneity in weight compared to normal wildtype siblings (30% to 90%). There is some pre-weaning loss. Homozygotes succumb to renal failure as a result of polycystic kidney disease. Hematopoiesis, including lymphocyte differentiation is initially normal but the thymus and spleen decrease in size due to increased apoptosis. The coat color of homozygotes turns grey with the second hair follicle cycle.
|Allele Name||targeted mutation 1, Stanley J Korsmeyer|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Bcl-2-; BclIItm1Sjk|
|Gene Symbol and Name||Bcl2, B cell leukemia/lymphoma 2|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A 1.1 kb genomic fragment containing all of the coding region of exon 3 was replaced with a neomycin selection cassette. The authors state that no full length or truncated protein was detected in vitro or in vivo (data not shown).|
|Mutations Made By|| |
Dr. Stanley Korsmeyer, Dana-Farber Cancer Institute
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).Some homozygotes due due to growth retardation and renal failure.
When using the bcl-2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002265 in your Materials and Methods section.