Overview

Also Known As:ragged Jackson

These mice carry a spontaneous re-mutation at the Sox18 locus with a similar phenotype to the Sox18^Ra mutation. Heterozygotes have aberrant hair follicles and abnormal development of the coat. Homozygotes are nearly bald, lack vibrissae, and usually die before weaning.

Genetic overview

Genetic Background	Generation

Sox18^Ra-J

Allele Type	Gene Symbol	Gene Name
Spontaneous	Sox18	SRY (sex determining region Y)-box 18

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Research Applications

Dermatology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The Sox18^Ra and Sox18^Ra-J alleles cause a less severe phenotype than the Sox18^Ra-Op allele. The Sox18^Ra and Sox18^Ra-J alleles are similar mutations and give a very similar phenotype. The Sox18^Ra allele has been more broadly described in the literature and will be covered here. Heterozygotes are viable and fertile. Heterozygotes have developmentally retarded sinus hair growth apparent at embryonic day 16.5 and retarded development of pelage follicles apparent by embryonic day 17.5. Thus, heterozygotes have slightly shorter vibrissae evident at birth, and can be distinguished at three days of age by their pink skin which, due to the abnormally sparse development of the coat, fails to darken like that of wildtype siblings. A paucity of fur is apparent by nine days of age and persists throughout life. Compared with the wild type pelage, Sox18^Ra/+ coats have longer guard hairs, shorter awls and zigzags, an increased number of guard hairs and awls, fewer zigzags, and no auchenes. There are mild morphological abnormalities in the hairs. There is no decrease in the number of hair follicles, but many of the follicles fail to grow hair. There is decreased yellow pigment in the hair causing the thin coat that develops to be darker than normal particularly in the dorsal midline. Subsequent to the first wave, hair growth is asynchronous and the normal cyclic fluctuations in skin thickness are not found. The adipose layer of the skin is thinner than normal. Despite this asynchrony of adjacent hair follicles, hair cycles do occur across the pelage, but are more diffuse than normal. The hair follicles have an aberrant shape and orientation. This aberrancy is more pronounced in homozygotes. The impact of the Sox18^Ra mutation on hair is more pronounced in the anterior regions than in the posterior regions. Approximately one in ten heterozygous pups displays chylous ascites, and the most severely affected do not survive. This trait is seen in males more than in females and is modified by genetic background. (Carter and Phillips, 1954; Slee, 1956 and 1957; Mann, 1963; Herbertson and Wallace, 1964; Wallace, 1979.)

Homozygotes are nearly bald, lack vibrissae, and usually die before weaning. They have generalized edema and weigh more at birth than wildtype littermates. It has been estimated that 40% of homozygotes die as embryos. The homozygotes that survive are often 5-10% shorter in body length. There are fewer hair follicles than normal and the few hairs that do grow have abnormal morphology. There is pigment in the tail and ear pinnae, and theear pinnae are thinner than normal and are often wrinkled. (Carter and Phillips, 1954; Slee, 1956 and 1957; Mann 1963.)

Genetics

Sox18^Ra-J

Allele Symbol: Sox18^Ra-J

Allele Name	ragged Jackson
Allele Type	Spontaneous
Allele Synonym(s)	Ra^J
Gene Symbol and Name	Sox18, SRY (sex determining region Y)-box 18
Gene Synonym(s)
Strain of Origin	C3H/HeSnJ
Chromosome	2
Molecular Note	A deletion of a guanine residue introduced a frameshift mutation affecting amino acids downstream of 313. Translation was prematurely stopped at codon 435. The deleted nucleotide was reported as nucleotide 959 in J:61488, 970 in J:74211, and nucleotide 937 in J:83731.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Sox18^Ra-J related

Dermatology Research
- Skin and Hair Texture Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Sox18^Ra-J/Sox18⁺
involves: C3H/HeSnJ

integument phenotype

darkened coat color
- agouti pattern is modified, the entire coat being unusually dark

retarded hair growth
- the first coat develops a little more slowly than normal

short vibrissae
- at birth and in juvenile mice vibrissae are often shorter than seen in littermate controls; there is no noteable difference in adult mice

sparse hair
- the first hair coat is thin, ragged in appearance

pigmentation phenotype

darkened coat color
- agouti pattern is modified, the entire coat being unusually dark

Genotype: Sox18^Ra-J/Sox18^Ra-J
involves: C3H/HeSnJ

growth/size/body region phenotype

decreased body size
- infrequent survivors are runted

integument phenotype

abnormal vibrissa number
- few if any vibrissae are seen

hairless

mortality/aging

neonatal lethality, incomplete penetrance
- the majority of mice die shortly after birth

References

Additional References

Dunn TL; Mynett-Johnson L; Wright EM; Hosking BM; Koopman PA; Muscat GE. 1995. Sequence and expression of Sox-18 encoding a new HMG-box transcription factor. Gene 161(2):223-5PubMed: 7665083 MGI: J:28369
Hosking BM; Wyeth JR; Pennisi DJ; Wang SC; Koopman P; Muscat GE. 2001. Cloning and functional analysis of the Sry-related HMG box gene, Sox18. Gene 262(1-2):239-47PubMed: 11179689 MGI: J:67559
James K; Hosking B; Gardner J; Muscat GE; Koopman P. 2003. Sox18 mutations in the ragged mouse alleles ragged-like and opossum. Genesis 36(1):1-6PubMed: 12748961 MGI: J:83731
Pennisi D; Bowles J; Nagy A; Muscat G; Koopman P. 2000. Mice null for sox18 are viable and display a mild coat defect Mol Cell Biol 20(24):9331-6PubMed: 11094083 MGI: J:66010
Pennisi D; Gardner J; Chambers D; Hosking B; Peters J; Muscat G; Abbott C; Koopman P. 2000. Mutations in Sox18 underlie cardiovascular and hair follicle defects in ragged mice. Nat Genet 24(4):434-7PubMed: 10742113 MGI: J:61488
Wallace ME. 1979. Analysis of genetic control of chylous ascites in ragged mice. Heredity (Edinb) 43(1):9-18PubMed: 291594 MGI: J:6220

Additional - Sox18^Ra-J related

Technical Support

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Genotyping Protocols
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Citation
When using the ragged Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #002261 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Wild-type for Sox18<Ra-J>

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:ragged Jackson

Genetic overview

Research Applications

Base Price

Detailed Description

Sox18Ra-J

Allele Symbol: Sox18Ra-J

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Sox18Ra-J related

Mammalian Phenotype Terms by Genotype

Genotype: Sox18Ra-J/Sox18+involves: C3H/HeSnJ

integument phenotype

pigmentation phenotype

Genotype: Sox18Ra-J/Sox18Ra-Jinvolves: C3H/HeSnJ

growth/size/body region phenotype

integument phenotype

mortality/aging

References

Additional References

Additional - Sox18Ra-J related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Sox18^Ra-J

Allele Symbol: Sox18^Ra-J

Genotype: Sox18^Ra-J related

Genotype: Sox18^Ra-J/Sox18⁺
involves: C3H/HeSnJ

Genotype: Sox18^Ra-J/Sox18^Ra-J
involves: C3H/HeSnJ

Additional - Sox18^Ra-J related