JAX
Mouse Strain Datasheet - 002228
C3.129P2(B6)-Il2tm1Hor/J
Also Known As: IL-2-
Homozygous mutant mice for the Il2tm1Hor targeted mutation show no apparent deficit in thymocyte differentiation or selection, types and numbers of T cells from spleens and lymph nodes are comparable to those of wildtype. They do have an impaired response to polyclonal T cell activators in the absence of additional IL2, deficits in their helper function and a reduction in natural killer cell activity. There is significant pre-weaning and post-weaning loss of homozygotes on the C3H/HeJCrlBR and C57BL/6J genetic backgrounds. In addition, homozygous mice develop inflammatory bowel disease between 6 and 15 weeks of age and reportedly die within 10-25 weeks under conventional housing conditions. Homozygotes on the C57BL/6J genetic background show an atrophied pancreas with apparently intact islets. Homozygotes on the BALB/c genetic background do not develop inflammatory bowel disease symptoms but rather die 3-5 weeks postnatally of a form of hemolytic anemia. For a more de...
Donating Investigator
Dr. Ivan Horak, Forschungs institut fur Molekulare
Genetic overview
| Genetic Background | Generation |
|---|---|
|
000659 C3H/HeJ |
|
Il2tm1Hor
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Targeted (Null/Knockout) | Il2 | interleukin 2 |
Research Applications
- Immunology, Inflammation and Autoimmunity Research
- Cancer Research
- Hematological Research
Detailed Description
Homozygous mutant mice for the Il2tm1Hor targeted mutation show no apparent deficit in thymocyte differentiation or selection, types and numbers of T cells from spleens and lymph nodes are comparable to those of wildtype. They do have an impaired response to polyclonal T cell activators in the absence of additional IL2, deficits in their helper function and a reduction in natural killer cell activity. There is significant pre-weaning and post-weaning loss of homozygotes on the C3H/HeJCrlBR and C57BL/6J genetic backgrounds. In addition, homozygous mice develop inflammatory bowel disease between 6 and 15 weeks of age and reportedly die within 10-25 weeks under conventional housing conditions. Homozygotes on the C57BL/6J genetic background show an atrophied pancreas with apparently intact islets. Homozygotes on the BALB/c genetic background do not develop inflammatory bowel disease symptoms but rather die 3-5 weeks postnatally of a form of hemolytic anemia. For a more detailed description please refer to the JAX Notes Fall 1996 article.
Control Suggestions
Selected References
- Schorle H; Holtschke T; Hunig T; Schimpl A; Horak I. 1991. Development and function of T cells in mice rendered interleukin-2 deficient by gene targeting. Nature 352(6336):621-4. PubMed: 1830926MGI: J:39989
Il2tm1Hor
Allele Symbol: Il2tm1Hor
| Allele Name | targeted mutation 1, Ivan Horak |
|---|---|
| Allele Type | Targeted (Null/Knockout) |
| Allele Synonym(s) | IL-2 KO; IL-2-; IL-2null; Il2- |
| Gene Symbol and Name | Il2, interleukin 2 |
| Gene Synonym(s) | IL-2; Il-2; TCGF; lymphokine |
| Strain of Origin | 129P2/OlaHsd |
| Chromosome | 3 |
| General Note | Phenotypic Similarity to Human Syndrome: Inflammatory Bowel Disease (J:15223).Phenotypic Similarity to Human Syndrome: Inflammatory Bowel Disease (J:51450) with B2m |
| Molecular Note | Insertion of a neomycin-resistance gene into the third exon introduced several stop codons in all reading frames. |
| Mutations Made By | Dr. Ivan Horak, Forschungs institut fur Molekulare |
Disease Terms
Research Areas By Genotype
This mouse can be used to support research in many areas including:
- Immunology, Inflammation and Autoimmunity Research
- Inflammation
- Inflammatory bowel disease
- Inflammation
Genotype: Il2tm1Hor related
- Cancer Research
- Growth Factors/Receptors/Cytokines
- Hematological Research
- Anemia, Iron Deficiency and Transport Defects
- hemolytic
- Immunological Defects
- Anemia, Iron Deficiency and Transport Defects
- Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
- hemolytic anemia
- Growth Factors/Receptors/Cytokines
- Autoimmunity
Mammalian Phenotype Terms by Genotype
Genotype: Il2tm1Hor/Il2tm1Hor
C3.129P2-Il2tm1Hor
hematopoietic system phenotype
- increased CD4-positive, alpha beta T cell number
- activated T cell numbers fail to decrease to normal levels after immunization with staphylococcal enterotoxin, indicating a problem with peripheral deletion (MGI Ref ID J:28924)
- increased CD8-positive, alpha-beta T cell number
- activated T cell numbers decrease with slower kinetics than wild-type mice after after immunization with staphylococcal enterotoxin (MGI Ref ID J:28924)
immune system phenotype
- increased CD4-positive, alpha beta T cell number
- activated T cell numbers fail to decrease to normal levels after immunization with staphylococcal enterotoxin, indicating a problem with peripheral deletion (MGI Ref ID J:28924)
- increased CD8-positive, alpha-beta T cell number
- activated T cell numbers decrease with slower kinetics than wild-type mice after after immunization with staphylococcal enterotoxin (MGI Ref ID J:28924)
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain
Genotype: Il2tm1Hor/Il2tm1Hor
B6.129P2-Il2tm1Hor/J
digestive/alimentary phenotype
- abnormal salivary gland duct morphology
- mild ductal changes in the salivary gland (MGI Ref ID J:125129)
- abnormal salivary gland morphology
- severe acinar gland destruction (MGI Ref ID J:125129)
- decreased salivation
- mice exhibit an increase in the lag time and a reduction of saliva production when treated with Pilocarpine, indicating loss of salivary gland function (MGI Ref ID J:125129)
- large intestinal inflammation
- salivary gland inflammation
- although more Gr-1+ cells are seen in the salivary glands, the absolute number of neutrophils is relatively small (MGI Ref ID J:125129)
- major population of infiltrating leukocytes are lymphocytes and to a much lower extent neutrophils (MGI Ref ID J:125129)
- salivary glands show presence of a large fraction of T cells and a ratio of CD4+/CD8+ of 1/2 (MGI Ref ID J:125129)
- severe inflammation in the salivary glands (MGI Ref ID J:125129)
immune system phenotype
- abnormal T cell activation
- both CD4+ and CD8+ T cells fail to increase in size and granularity after activation with anti-CD3/28 (MGI Ref ID J:190168)
- abnormal T cell proliferation
- DNA replication fails to increase in activated CD4+ and CD8+ T cells after activation with anti-CD3/28 (MGI Ref ID J:190168)
- abnormal circulating cytokine level
- (MGI Ref ID J:190168)
- abnormal response to transplant
- wild-type regulatory T cells fail to engraft when adoptively transferred into these mice (MGI Ref ID J:113547)
- decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number
- 4-fold (MGI Ref ID J:131774)
- decreased circulating interferon-gamma level
- after activation with anti-CD3/28 (MGI Ref ID J:190168)
- decreased circulating interleukin-13 level
- after activation with anti-CD3/28 (MGI Ref ID J:190168)
- decreased circulating interleukin-4 level
- after activation with anti-CD3/28 (MGI Ref ID J:190168)
- decreased regulatory T cell number
- there is a 2 to 5 fold reduction in the percentage of CD25-postive CD4 T cells in both the thymus and lymph nodes (MGI Ref ID J:113547)
- enlarged lymph nodes
- increased cellularity of lymph nodes compared to wild-type mice, but lower than what is observed in Il2rbtm1Mak mice (MGI Ref ID J:113547)
- increased CD4-positive, alpha beta T cell number
- 3-fold (MGI Ref ID J:131774)
- increased splenocyte number
- (MGI Ref ID J:131774)
- increased susceptibility to autoimmune disorder
- lacrimal gland inflammation
- severe inflammation in the lacrimal glands (MGI Ref ID J:125129)
- large intestinal inflammation
- salivary gland inflammation
- although more Gr-1+ cells are seen in the salivary glands, the absolute number of neutrophils is relatively small (MGI Ref ID J:125129)
- major population of infiltrating leukocytes are lymphocytes and to a much lower extent neutrophils (MGI Ref ID J:125129)
- salivary glands show presence of a large fraction of T cells and a ratio of CD4+/CD8+ of 1/2 (MGI Ref ID J:125129)
- severe inflammation in the salivary glands (MGI Ref ID J:125129)
endocrine/exocrine gland phenotype
- abnormal salivary gland duct morphology
- mild ductal changes in the salivary gland (MGI Ref ID J:125129)
- abnormal salivary gland morphology
- severe acinar gland destruction (MGI Ref ID J:125129)
- decreased salivation
- mice exhibit an increase in the lag time and a reduction of saliva production when treated with Pilocarpine, indicating loss of salivary gland function (MGI Ref ID J:125129)
- lacrimal gland inflammation
- severe inflammation in the lacrimal glands (MGI Ref ID J:125129)
- salivary gland inflammation
- although more Gr-1+ cells are seen in the salivary glands, the absolute number of neutrophils is relatively small (MGI Ref ID J:125129)
- major population of infiltrating leukocytes are lymphocytes and to a much lower extent neutrophils (MGI Ref ID J:125129)
- salivary glands show presence of a large fraction of T cells and a ratio of CD4+/CD8+ of 1/2 (MGI Ref ID J:125129)
- severe inflammation in the salivary glands (MGI Ref ID J:125129)
cellular phenotype
- abnormal cellular respiration
- fail to switch from oxidative phosphorylation to aerobic glycolysis after 5 days of tamoxifen treatment and activation with anti-CD3/28 (MGI Ref ID J:190168)
vision/eye phenotype
- lacrimal gland inflammation
- severe inflammation in the lacrimal glands (MGI Ref ID J:125129)
homeostasis/metabolism phenotype
- abnormal circulating cytokine level
- (MGI Ref ID J:190168)
- decreased circulating interferon-gamma level
- after activation with anti-CD3/28 (MGI Ref ID J:190168)
- decreased circulating interleukin-13 level
- after activation with anti-CD3/28 (MGI Ref ID J:190168)
- decreased circulating interleukin-4 level
- after activation with anti-CD3/28 (MGI Ref ID J:190168)
- decreased salivation
- mice exhibit an increase in the lag time and a reduction of saliva production when treated with Pilocarpine, indicating loss of salivary gland function (MGI Ref ID J:125129)
hematopoietic system phenotype
- abnormal T cell activation
- both CD4+ and CD8+ T cells fail to increase in size and granularity after activation with anti-CD3/28 (MGI Ref ID J:190168)
- abnormal T cell proliferation
- DNA replication fails to increase in activated CD4+ and CD8+ T cells after activation with anti-CD3/28 (MGI Ref ID J:190168)
- decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number
- 4-fold (MGI Ref ID J:131774)
- decreased regulatory T cell number
- there is a 2 to 5 fold reduction in the percentage of CD25-postive CD4 T cells in both the thymus and lymph nodes (MGI Ref ID J:113547)
- increased CD4-positive, alpha beta T cell number
- 3-fold (MGI Ref ID J:131774)
- increased splenocyte number
- (MGI Ref ID J:131774)
Genotype: Il2tm1Hor/Il2tm1Hor
B6.129P2-Il2tm1Hor
immune system phenotype
- immune system phenotype
- bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) do not develop a wasting autoimmune disease compared to the lethal disease that develops with Il2ratm1Dw bone marrow chimerasmarrow chimeras (MGI Ref ID J:125748)
- increased CD4-positive, alpha beta T cell number
- activated T cell numbers fail to decrease to normal levels after immunization with staphylococcal enterotoxin, indicating a problem with peripheral deletion (MGI Ref ID J:28924)
- increased CD8-positive, alpha-beta T cell number
- activated T cell numbers decrease with slower kinetics than wild-type mice after after immunization with staphylococcal enterotoxin (MGI Ref ID J:28924)
- increased T cell number
- (MGI Ref ID J:125748)
- increased T cell proliferation
- T cells continue to respond to antigen instead of going into anergy during secondary stimulation with antigens (MGI Ref ID J:28924)
- increased activated T cell number
- higher numbers due to less sensitivity to Fas-mediated cell death (apoptosis) (MGI Ref ID J:28924)
hematopoietic system phenotype
- increased CD4-positive, alpha beta T cell number
- activated T cell numbers fail to decrease to normal levels after immunization with staphylococcal enterotoxin, indicating a problem with peripheral deletion (MGI Ref ID J:28924)
- increased CD8-positive, alpha-beta T cell number
- activated T cell numbers decrease with slower kinetics than wild-type mice after after immunization with staphylococcal enterotoxin (MGI Ref ID J:28924)
- increased T cell number
- (MGI Ref ID J:125748)
- increased T cell proliferation
- T cells continue to respond to antigen instead of going into anergy during secondary stimulation with antigens (MGI Ref ID J:28924)
- increased activated T cell number
- higher numbers due to less sensitivity to Fas-mediated cell death (apoptosis) (MGI Ref ID J:28924)
Genotype: Il2tm1Hor/Il2tm1Hor
involves: 129P2/OlaHsd * C57BL/6J
immune system phenotype
- increased T-helper 17 cell number
- 30-fold difference in absolute numbers of mesenteric Th17 cells compared with wild-type mice (MGI Ref ID J:120176)
- greater proportion of IL-17-positive cells (most of which CD4 positive) in peripheral lymph nodes (MGI Ref ID J:120176)
- increase in the percentage of IL-17-producing cells in culturing CD4+ T cells in vitro under Th17-polarizing conditions (MGI Ref ID J:120176)
- increased circulating interleukin-17 level
- elevated serum IL-17 concentration at 3 months old (MGI Ref ID J:120176)
homeostasis/metabolism phenotype
- increased circulating interleukin-17 level
- elevated serum IL-17 concentration at 3 months old (MGI Ref ID J:120176)
hematopoietic system phenotype
- increased T-helper 17 cell number
- 30-fold difference in absolute numbers of mesenteric Th17 cells compared with wild-type mice (MGI Ref ID J:120176)
- greater proportion of IL-17-positive cells (most of which CD4 positive) in peripheral lymph nodes (MGI Ref ID J:120176)
- increase in the percentage of IL-17-producing cells in culturing CD4+ T cells in vitro under Th17-polarizing conditions (MGI Ref ID J:120176)
Genotype: Il2tm1Hor/Il2tm1Hor
C.129P2-Il2tm1Hor
mortality/aging
- premature death
- Background Sensitivity - mice all die by 5 weeks of age, compared to death by 25 weeks of age for mice homozygous for the same mutant locus on a B6.129P2 background (MGI Ref ID J:29799)
- mice all die by 5 weeks of age, compared to death by 25 weeks of age for mice with the same mutant locus on a mixed genetic background (MGI Ref ID J:29799)
immune system phenotype
- abnormal lymphocyte morphology
- (MGI Ref ID J:29799)
- abnormal regulatory T cell physiology
- there is a rapid loss of adoptively transferred regulatory T cells (MGI Ref ID J:112603)
- abnormal spleen morphology
- (MGI Ref ID J:29799)
- enlarged lymph nodes
- (MGI Ref ID J:29799)
- heart inflammation
- moderate to severe inflammation noted upon necropsy (MGI Ref ID J:29799)
- increased B cell proliferation
- faster proliferation as measured by BrdU incorporation (MGI Ref ID J:29799)
- increased IgG level
- increased level of IgG secreting B cells from spleen and lymph nodes (MGI Ref ID J:29799)
- increased T cell proliferation
- increased germinal center B cell number
- (MGI Ref ID J:29799)
- increased inflammatory response
- (MGI Ref ID J:29799)
- increased plasma cell number
- seen in the spleen by 15 days of age (MGI Ref ID J:29799)
- increased spleen red pulp amount
- (MGI Ref ID J:29799)
- increased susceptibility to autoimmune hemolytic anemia
- Background Sensitivity - cause of death for mice on this genetic background, as opposed to death caused by inflammatory bowel disease for homozygous mice on a B6.129P2 genetic background (MGI Ref ID J:29799)
- liver inflammation
- (MGI Ref ID J:29799)
- lung inflammation
- moderate to severe inflammation noted upon necropsy (MGI Ref ID J:29799)
- pancreas inflammation
- moderate to severe inflammation noted upon necropsy (MGI Ref ID J:29799)
- spleen hyperplasia
- 15 day old mice exhibit hyperplasia of the white pulp (MGI Ref ID J:29799)
- vasculitis
- moderate to severe inflammation noted in the major thoracic blood vessels upon necropsy (MGI Ref ID J:29799)
hematopoietic system phenotype
- abnormal bone marrow cell number
- invasion of T cells occur, with a concurrent drop in B cells and other nucleated cells (MGI Ref ID J:29799)
- abnormal lymphocyte morphology
- (MGI Ref ID J:29799)
- abnormal regulatory T cell physiology
- there is a rapid loss of adoptively transferred regulatory T cells (MGI Ref ID J:112603)
- abnormal reticulocyte morphology
- marked reduction concurrent with T cell invasion of marrow (MGI Ref ID J:29799)
- abnormal spleen morphology
- (MGI Ref ID J:29799)
- decreased hematocrit
- (MGI Ref ID J:29799)
- increased B cell proliferation
- faster proliferation as measured by BrdU incorporation (MGI Ref ID J:29799)
- increased IgG level
- increased level of IgG secreting B cells from spleen and lymph nodes (MGI Ref ID J:29799)
- increased T cell proliferation
- increased germinal center B cell number
- (MGI Ref ID J:29799)
- increased mean corpuscular hemoglobin
- about twice normal levels, this indicates a hyperchromic anemia (MGI Ref ID J:29799)
- increased plasma cell number
- seen in the spleen by 15 days of age (MGI Ref ID J:29799)
- increased spleen red pulp amount
- (MGI Ref ID J:29799)
- increased susceptibility to autoimmune hemolytic anemia
- Background Sensitivity - cause of death for mice on this genetic background, as opposed to death caused by inflammatory bowel disease for homozygous mice on a B6.129P2 genetic background (MGI Ref ID J:29799)
- low mean erythrocyte cell number
- (MGI Ref ID J:29799)
- spleen hyperplasia
- 15 day old mice exhibit hyperplasia of the white pulp (MGI Ref ID J:29799)
homeostasis/metabolism phenotype
- increased circulating bilirubin level
- (MGI Ref ID J:29799)
cardiovascular system phenotype
- heart inflammation
- moderate to severe inflammation noted upon necropsy (MGI Ref ID J:29799)
- vasculitis
- moderate to severe inflammation noted in the major thoracic blood vessels upon necropsy (MGI Ref ID J:29799)
endocrine/exocrine gland phenotype
- pancreas inflammation
- moderate to severe inflammation noted upon necropsy (MGI Ref ID J:29799)
liver/biliary system phenotype
- liver inflammation
- (MGI Ref ID J:29799)
respiratory system phenotype
- lung inflammation
- moderate to severe inflammation noted upon necropsy (MGI Ref ID J:29799)
Genotype: Il2tm1Hor/Il2tm1Hor
involves: 129P2/OlaHsd * C57BL/6
mortality/aging
- premature death
cellular phenotype
- decreased splenocyte proliferation
- poor proliferative response to anti-CD3 antibody is partially rescued by addition of cytokines including IL-2, IL-4, IL-7 (MGI Ref ID J:79241)
- proliferative response of splenocytes to anti-CD3 antibody is only 16% of wt controls (MGI Ref ID J:79241)
- there is reduced proliferation to allogenic leukocytes (MGI Ref ID J:79241)
digestive/alimentary phenotype
- abnormal colon goblet cell morphology
- abnormal intestinal epithelium morphology
- chronic diarrhea
- all mice that do not die within the first 9 weeks after birth exhibit diarrhea (MGI Ref ID J:15223)
- colitis
- all mice that do not die within the first 9 weeks after birth develop an inflammatory bowel disease that is similar to the human disease ulcerative colitis (MGI Ref ID J:15223)
- infiltrating lymphocytes sometimes form nodules (MGI Ref ID J:15223)
- mice bred in a germ-free facility do not display any histopathological signs of colitis (MGI Ref ID J:15223)
- mice bred under specific pathogen free conditions do not develop any clinical signs of colitis but histological and immunological examinations show the beginning of inflammatory processes in mice that are 17-20 weeks of age (MGI Ref ID J:15223)
- mice kept under a specific pathogen free environment develop colitis when immunized with an antigen that activates CD4 T cells (MGI Ref ID J:37220)
- occurs in all mice that survive past 9 weeks of age, with thickening of large intestinal wall (MGI Ref ID J:29999)
- crypts of Lieberkuhn abscesses
- frequently occurres in the large intestine (MGI Ref ID J:15223)
- diarrhea
- occurs in mice 24 weeks of age (MGI Ref ID J:29999)
- intestinal ulcer
- ulcers occur in the large intestine, with pronounced thickening of the bowel wall (MGI Ref ID J:15223)
- rectal hemorrhage
- rarely observed (MGI Ref ID J:29999)
- rectal prolapse
immune system phenotype
- abnormal CD4-positive, alpha-beta T cell physiology
- however, IgM levels are in vivo are normal (MGI Ref ID J:39989)
- increased cytotoxic effectiveness of CD4 T cells isolated from the colon (MGI Ref ID J:39981)
- there is an impaired ability to induce Ig class switching in B cells after infection with vesicular stomatitis virus (MGI Ref ID J:15573)
- unable to induce IgM secretion from activated B cells in an in vitro assay (MGI Ref ID J:39989)
- abnormal CD8 positive, alpha-beta intraepithelial T cell morphology
- abnormal T-helper 1 cell differentiation
- 10 fold greater amounts of IFN gamma were made by thymocytes from immunized mutant mice as measured by an in vitro assay (MGI Ref ID J:37220)
- however, treatment with an IL-12 neutralizing antibody normalized Th1cytokine levels (MGI Ref ID J:37220)
- low levels of the Th2 cytokine IL-4 were made compared to the large level made by wt thymocytes (MGI Ref ID J:37220)
- abnormal class switch recombination
- there is delayed class switching from IgM to IgG after infection with vesicular stomatitis virus (MGI Ref ID J:15573)
- abnormal cytotoxic T cell physiology
- abnormal gamma-delta intraepithelial T cell morphology
- decreased percentage of these cells are found in the colon epithelium (MGI Ref ID J:15223)
- abnormal level of surface class II molecules
- expression occurs in high number of colon epithelial cells (MGI Ref ID J:15223)
- abnormal lymphocyte cell number
- (MGI Ref ID J:15223)
- abnormal lymphocyte morphology
- decreased apoptosis in thymus upon injection with anti-CD3 antibody (MGI Ref ID J:37220)
- abnormal lymphocyte physiology
- (MGI Ref ID J:15223)
- abnormal thymocyte activation
- however, treatment with an IL-12 neutralizing antibody normalized the percentage of activated thymocytes (MGI Ref ID J:37220)
- in vitro proliferation is reduced in presence of concanavalin A or anti-CD3 antibody (MGI Ref ID J:39989)
- increased activation of thymocytes 7 days after immunization with a CD4 T cell activating antigen, as measured by expression of CD69 and Icam1 (MGI Ref ID J:37220)
- colitis
- all mice that do not die within the first 9 weeks after birth develop an inflammatory bowel disease that is similar to the human disease ulcerative colitis (MGI Ref ID J:15223)
- infiltrating lymphocytes sometimes form nodules (MGI Ref ID J:15223)
- mice bred in a germ-free facility do not display any histopathological signs of colitis (MGI Ref ID J:15223)
- mice bred under specific pathogen free conditions do not develop any clinical signs of colitis but histological and immunological examinations show the beginning of inflammatory processes in mice that are 17-20 weeks of age (MGI Ref ID J:15223)
- mice kept under a specific pathogen free environment develop colitis when immunized with an antigen that activates CD4 T cells (MGI Ref ID J:37220)
- occurs in all mice that survive past 9 weeks of age, with thickening of large intestinal wall (MGI Ref ID J:29999)
- decreased T cell proliferation
- (MGI Ref ID J:26198)
- decreased cytotoxic T cell cytolysis
- footpad swelling is reduced 6-10 days after infection with lymphocytic choriomeningitis virus (MGI Ref ID J:26861)
- no target cell killing by previously stimulated T cells in a chromium release assay (MGI Ref ID J:15573)
- there is a three fold reduction of target cell killing by primary T cells in a chromium release assay (MGI Ref ID J:15573)
- there is reduced killing of allogeneic tumor cells in an in vitro assay (MGI Ref ID J:26198)
- decreased double-negative T cell number
- there is a drastic reduction in the colon (MGI Ref ID J:15223)
- decreased double-positive T cell number
- percentage of these cells is decreased (47.0% vs 86.2%) in the thymus when mice are immunized with a CD4 T cell activating antigen (MGI Ref ID J:37220)
- decreased splenocyte proliferation
- poor proliferative response to anti-CD3 antibody is partially rescued by addition of cytokines including IL-2, IL-4, IL-7 (MGI Ref ID J:79241)
- proliferative response of splenocytes to anti-CD3 antibody is only 16% of wt controls (MGI Ref ID J:79241)
- there is reduced proliferation to allogenic leukocytes (MGI Ref ID J:79241)
- enlarged lymph nodes
- (MGI Ref ID J:15223)
- enlarged mesenteric lymph nodes
- increased 3-10 fold in size as compared to control littermates (MGI Ref ID J:29999)
- enlarged spleen
- occurs in the 50% of mice that die prematurely (MGI Ref ID J:15223)
- impaired natural killer cell mediated cytotoxicity
- 3 to 9 fold reduction in killing of NK susceptible target cells as measured by chromium release assay (MGI Ref ID J:15573)
- increased B cell proliferation
- B cells from lymph nodes have a larger size and incorporate more BrdU, indicating a faster proliferation rate (MGI Ref ID J:16662)
- increased IgA level
- drastic elevation in secretion by B cells found in the colon (MGI Ref ID J:15223)
- increased IgE level
- levels are 10 fold higher than those observed in wild-type mice (MGI Ref ID J:16662)
- increased IgG1 level
- increased IgG2a level
- levels of IgG2a are also elevated (MGI Ref ID J:16662)
- increased IgG2b level
- levels of IgG2b are also elevated (MGI Ref ID J:16662)
- increased T cell proliferation
- increased double-negative T cell number
- percentage of these cells is increased (11.2% vs 3.7%) in the thymus when mice are immunized with a CD4 T cell activating antigen (MGI Ref ID J:37220)
- increased length of allograft survival
- graft rejection of allogeneic pancreatic islet cells is delayed by 11 days compared to controls (MGI Ref ID J:26198)
- increased pre-B cell number
- of cells in the bone marrow (MGI Ref ID J:16662)
- increased pro-B cell number
- of cells in the bone marrow (MGI Ref ID J:16662)
- increased single-positive T cell number
- increased susceptibility to viral infection
- despite reduced cytotoxic T cell activity, mice still clear lymphocytic choriomeningitis virus within nine days of infection (MGI Ref ID J:26861)
- lymph node hyperplasia
- (MGI Ref ID J:16662)
growth/size/body region phenotype
- cachexia
- weight loss resulting from intestinal inflammation is observed by 24 weeks (MGI Ref ID J:29999)
hematopoietic system phenotype
- abnormal CD4-positive, alpha-beta T cell physiology
- however, IgM levels are in vivo are normal (MGI Ref ID J:39989)
- increased cytotoxic effectiveness of CD4 T cells isolated from the colon (MGI Ref ID J:39981)
- there is an impaired ability to induce Ig class switching in B cells after infection with vesicular stomatitis virus (MGI Ref ID J:15573)
- unable to induce IgM secretion from activated B cells in an in vitro assay (MGI Ref ID J:39989)
- abnormal CD8 positive, alpha-beta intraepithelial T cell morphology
- abnormal T-helper 1 cell differentiation
- 10 fold greater amounts of IFN gamma were made by thymocytes from immunized mutant mice as measured by an in vitro assay (MGI Ref ID J:37220)
- however, treatment with an IL-12 neutralizing antibody normalized Th1cytokine levels (MGI Ref ID J:37220)
- low levels of the Th2 cytokine IL-4 were made compared to the large level made by wt thymocytes (MGI Ref ID J:37220)
- abnormal class switch recombination
- there is delayed class switching from IgM to IgG after infection with vesicular stomatitis virus (MGI Ref ID J:15573)
- abnormal cytotoxic T cell physiology
- abnormal gamma-delta intraepithelial T cell morphology
- decreased percentage of these cells are found in the colon epithelium (MGI Ref ID J:15223)
- abnormal level of surface class II molecules
- expression occurs in high number of colon epithelial cells (MGI Ref ID J:15223)
- abnormal lymphocyte cell number
- (MGI Ref ID J:15223)
- abnormal lymphocyte morphology
- decreased apoptosis in thymus upon injection with anti-CD3 antibody (MGI Ref ID J:37220)
- abnormal lymphocyte physiology
- (MGI Ref ID J:15223)
- abnormal thymocyte activation
- however, treatment with an IL-12 neutralizing antibody normalized the percentage of activated thymocytes (MGI Ref ID J:37220)
- in vitro proliferation is reduced in presence of concanavalin A or anti-CD3 antibody (MGI Ref ID J:39989)
- increased activation of thymocytes 7 days after immunization with a CD4 T cell activating antigen, as measured by expression of CD69 and Icam1 (MGI Ref ID J:37220)
- anemia
- decreased T cell proliferation
- (MGI Ref ID J:26198)
- decreased cytotoxic T cell cytolysis
- footpad swelling is reduced 6-10 days after infection with lymphocytic choriomeningitis virus (MGI Ref ID J:26861)
- no target cell killing by previously stimulated T cells in a chromium release assay (MGI Ref ID J:15573)
- there is a three fold reduction of target cell killing by primary T cells in a chromium release assay (MGI Ref ID J:15573)
- there is reduced killing of allogeneic tumor cells in an in vitro assay (MGI Ref ID J:26198)
- decreased double-negative T cell number
- there is a drastic reduction in the colon (MGI Ref ID J:15223)
- decreased double-positive T cell number
- percentage of these cells is decreased (47.0% vs 86.2%) in the thymus when mice are immunized with a CD4 T cell activating antigen (MGI Ref ID J:37220)
- decreased splenocyte proliferation
- poor proliferative response to anti-CD3 antibody is partially rescued by addition of cytokines including IL-2, IL-4, IL-7 (MGI Ref ID J:79241)
- proliferative response of splenocytes to anti-CD3 antibody is only 16% of wt controls (MGI Ref ID J:79241)
- there is reduced proliferation to allogenic leukocytes (MGI Ref ID J:79241)
- enlarged spleen
- occurs in the 50% of mice that die prematurely (MGI Ref ID J:15223)
- impaired natural killer cell mediated cytotoxicity
- 3 to 9 fold reduction in killing of NK susceptible target cells as measured by chromium release assay (MGI Ref ID J:15573)
- increased B cell proliferation
- B cells from lymph nodes have a larger size and incorporate more BrdU, indicating a faster proliferation rate (MGI Ref ID J:16662)
- increased IgA level
- drastic elevation in secretion by B cells found in the colon (MGI Ref ID J:15223)
- increased IgE level
- levels are 10 fold higher than those observed in wild-type mice (MGI Ref ID J:16662)
- increased IgG1 level
- increased IgG2a level
- levels of IgG2a are also elevated (MGI Ref ID J:16662)
- increased IgG2b level
- levels of IgG2b are also elevated (MGI Ref ID J:16662)
- increased T cell proliferation
- increased double-negative T cell number
- percentage of these cells is increased (11.2% vs 3.7%) in the thymus when mice are immunized with a CD4 T cell activating antigen (MGI Ref ID J:37220)
- increased pre-B cell number
- of cells in the bone marrow (MGI Ref ID J:16662)
- increased pro-B cell number
- of cells in the bone marrow (MGI Ref ID J:16662)
- increased single-positive T cell number
endocrine/exocrine gland phenotype
- abnormal colon goblet cell morphology
- abnormal thymocyte activation
- however, treatment with an IL-12 neutralizing antibody normalized the percentage of activated thymocytes (MGI Ref ID J:37220)
- in vitro proliferation is reduced in presence of concanavalin A or anti-CD3 antibody (MGI Ref ID J:39989)
- increased activation of thymocytes 7 days after immunization with a CD4 T cell activating antigen, as measured by expression of CD69 and Icam1 (MGI Ref ID J:37220)
- crypts of Lieberkuhn abscesses
- frequently occurres in the large intestine (MGI Ref ID J:15223)
behavior/neurological phenotype
- hunched posture
- occurs in mice 24 weeks of age (MGI Ref ID J:29999)
cardiovascular system phenotype
- rectal hemorrhage
- rarely observed (MGI Ref ID J:29999)
homeostasis/metabolism phenotype
- amyloidosis
- predominately in liver, spleen, and kidneys (MGI Ref ID J:15223)
Genotype: Il2tm1Hor/Il2tm1Hor
involves: 129P2/OlaHsd
reproductive system phenotype
- reproductive system phenotype
- there are no abnormalities of the placenta, the mesometrial triangle, and differentiation of granulated metrial gland cells (aka uterine NK cells) in the uteri of pregnant mice at day 14 of gestation (MGI Ref ID J:134589)
immune system phenotype
- increased regulatory T cell number
- of CD25lo regulatory T cells (MGI Ref ID J:208318)
hematopoietic system phenotype
- increased regulatory T cell number
- of CD25lo regulatory T cells (MGI Ref ID J:208318)
References
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Additional References
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