These IL-2 knock-out mice exhibit an impaired response to polyclonal T cell activators, deficits in their helper function, and a reduction in natural killer cell activity.
Dr. Ivan Horak, Forschungs institut fur Molekulare
Genetic Background | Generation |
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000659 C3H/HeJ |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il2 | interleukin 2 |
Homozygous mutant mice for the Il2tm1Hor targeted mutation show no apparent deficit in thymocyte differentiation or selection, types and numbers of T cells from spleens and lymph nodes are comparable to those of wildtype. They do have an impaired response to polyclonal T cell activators in the absence of additional IL2, deficits in their helper function and a reduction in natural killer cell activity. There is significant pre-weaning and post-weaning loss of homozygotes on the BALB/c, C3H/HeJCrlBR and C57BL/6J genetic backgrounds. In addition, homozygous mice develop inflammatory bowel disease between 6 and 15 weeks of age and reportedly die within 10-25 weeks under conventional housing conditions. Homozygotes on the C57BL/6J genetic background show an atrophied pancreas with apparently intact islets. Homozygotes on the BALB/c genetic background do not develop inflammatory bowel disease symptoms but rather die 3-5 weeks postnatally of a form of hemolytic anemia. For a more detailed description please refer to the JAX Notes Fall 1996 article.
Allele Name | targeted mutation 1, Ivan Horak |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-2-; Il2-; IL-2 KO; IL-2null |
Gene Symbol and Name | Il2, interleukin 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 3 |
Molecular Note | Insertion of a neomycin-resistance gene into the third exon introduced several stop codons in all reading frames. |
Mutations Made By | Dr. Ivan Horak, Forschungs institut fur Molekulare |
When maintaining a live colony, heterozygous mice may be bred to wildtype siblings, or to C3H/HeJ inbred mice (Stock No. 000659).
When using the IL-2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002228 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Il2<tm1Hor> |
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