Mice homozygous for this mutation are viable and fertile although about 65% of homozygous mice die by 4 weeks of age unless maintained on allopurinol. Homozygous mice show severe hyperuricemia and urate nephropathy. These mice may serve as animal models for hyperuricemia and its related nephropathy in humans.
Dr. Allan Bradley, Baylor College of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Uox | urate oxidase |
Mice homozygous for this mutation are viable and fertile although about 65% of homozygous mice die by 4 weeks of age unless maintained on allopurinol. Homozygous mice show severe hyperuricemia and urate nephropathy. These mice may serve as animal models for hyperuricemia and its related nephropathy in humans.
Exon 3 of the Uox gene was disrupted using a vector containing the neo resistance gene. The 129-derived AB1 ES cell line was used.
Allele Name | targeted mutation 1, Baylor College of Medicine |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Uox, urate oxidase |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 3 |
Molecular Note | The insertion of a neomycin cassette into exon 3 disrupted the open reading frame at codon 107. Western blot analysis revealed an absence of encoded protein in liver extracts from homozygous mutant mice. Spectrographic analysis showing a lack of urate oxidase activity in liver extracts, confirmed functional ablation in homozygous mutant mice. |
Mutations Made By | Dr. Allan Bradley, Baylor College of Medicine |
When held in a live colony, this strain is maintained by homozygous sibling matings. The survival rate of homozygous mice can be improved with the administration of an Allopurinol therapy protocol.
When using the B6;129S7-Uoxtm1Bay/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002223 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Uox<tm1Bay> |
Frozen Mouse Embryo | B6;129S7-Uox<tm1Bay>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Uox<tm1Bay>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Uox<tm1Bay>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S7-Uox<tm1Bay>/J | $3373.50 |
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