These Tgfb1 knock-out mice exhibit early postnatal death and a reduced number of expected homozygous births.
IMR Colony, The Jackson Laboratory
It has been reported that mice homozygous for Tgfb1tm1Doe mutation develop normally but die approximately two to three weeks after birth. It was also reported that this strain shows a lower than expected number of homozygous births (~14% from heterozygous matings on a mixed genetic background) suggesting that in utero death may occur (See Husbandry). Homozygous mice usually die within a few days of the onset of a wasting syndrome. Death is due to a massive inflammatory cell response and tissue necrosis. A multifocal mixed inflammatory cell infiltration occurs into numerous organs, particularly the heart and stomach. Heterozygous mice do not differ from normal wildtype littermates. This strain may provide a model for human immune and inflammatory diseases such as autoimmune disease, transplant rejection and graft versus host reactions.
The observed recovery of homozygous mutants from heterozygote x heterozygote matings is 3.8% on the C57BL/6J genetic background. Homozygotes usually die by two to three weeks age. The literature suggests that a mixed or outbred genetic background can enhance the viability of homozygous knockout animals.
The Tgfb1tm1Doe mutant strain was developed in the laboratory of Dr. Tom Doetschman at the University of Cincinnati College of Medicine. The vector targeted neo insertion into exon 6 of the Tgfb1 gene. the 129-derived D3 ES cell line was used. The C57BL/6J strain was generated by backcrossing mice carrying the Tgfb1tm1Doe mutation 10 or more times to C57BL/6J inbred mice.
|Allele Name||targeted mutation 1, Thomas Doetschman|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Tgfb1-; TGFbeta1|
|Gene Symbol and Name||Tgfb1, transforming growth factor, beta 1|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin resistance cassette was inserted into exon 6 of the gene, disrupting the production of a mature peptide.|
|Mutations Made By|| |
Thomas Doetschman, University of Arizona
This Tgfb1tm1Doe strain is maintained by mating heterozygous mice to normal wildtype siblings. Heterozygous mice and normal wildtype siblings may be ordered. Our observed recovery of homozygous mutants from heterozygote x heterozygote matings is 3.8% on the C57BL/6J genetic background. Homozygotes usually die by two to three weeks age and therefore are not available. Expected coat color from breeding:Black
When using the TGF-β1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002220 in your Materials and Methods section.