Mice homozygous for the Tgfatm1Ard mutation develop normally, are of normal size, weight, and health, and are fertile. They display a pronounced waviness in the coat and whiskers, and dramatic derangement of the hair follicles. Older homozygous mice occasionally show some corneal inflammation that may be the result of a defect in wound healing.
The Tgfatm1Ard mutant strain was developed in the laboratory of Dr. Ashley Dunn at the Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch. The 129-derived E14 ES cell line was used. The C57BL/6J strain was generated by backcrossing mice carrying the Tgfatm1Ard mutation 5 or more times to C57BL/6J mice.
|Allele Name||targeted mutation 1, Ashley R Dunn|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Tgfalpha -|
|Gene Symbol and Name||Tgfa, transforming growth factor alpha|
|Strain of Origin||129P2/OlaHsd|
|General Note||In conjunction with Fgf7tm1Efu, double homozygous mutant mice display no additional abnormalities nor increase in severity of phenotypes over individual homozygous mutants. (J:31155)|
|Molecular Note||A neomycin cassette was inserted into exon 3 of the gene.|
|Mutations Made By|| |
Dr. Ashley Dunn, Ludwig Institute for Cancer Research
The Tgfatm1Ard strain is maintained by mating homozygous siblings. Homozygous mice may be ordered. Corneas of homozygous mutants are often scarred from injuries that do not heal normally. Expected coat color from breeding:Black
When using the TGFα - mouse strain in a publication, please cite the originating article(s) and include JAX stock #002219 in your Materials and Methods section.