C57BL/6-Col1a1^Mov13/J

Stock number: 002197
Product name: Mov-13
Research areas: Cardiovascular Research, Dermatology Research, Developmental Biology Research, Reproductive Biology Research

Description

These Mov-13 mice carry a single copy of the Moloney murine leukemia virus (MMuLV) stably integrated into intron 1 of the mouse Col1a1 (collagen, type I, alpha 1; also called Mov-13) gene. Heterozygotes can develop severe generalized lymphosarcoma at an early age.

Detailed description

These Mov-13 mice carry a single copy of the Moloney murine leukemia virus (MMuLV) stably integrated into intron 1 of the mouse (collagen, type I, alpha 1; also called Mov-13) gene, just 19 base pairs downstream of the intron/exon boundary. The insertion creates a complete transcriptional block in most cell types. Mice activate infectious virus during embryogenesis, leading to a distinct pattern of virus expression in all tissues of the adult.

When maintained as a live colony at The Jackson Laboratory, we noted significant casualties in heterozygotes due to severe generalized lymphosarcoma. Lymph nodes become enlarged, as are the liver and spleen. Kidneys and lungs may also be affected. Homozygous mice suffer from arrested development at embryonic day 12 and die at about embryonic day 13-14.

Heterozygous females are generally limited to producing a single litter (if any), thus the donating laboratory maintained their line by crossing heterozygous males with inbred/wildtype females.

Development

Full-length Moloney murine leukemia virus (MMuLV) DNA was injected into C57BL/6 embryos and a single copy was stably integrated intointron 1 of the mouse Col1a1 gene, just 19 base pairs downstream of the intron/exon boundary. This integration blocks transcription of the targeted gene in most cell types.

Allele

Symbol: Col1a1^Mov13
Name: Moloney leukemia virus 13
MGI accession ID: MGI:1856671
Generation method: Transgenic
Marker symbol: Col1a1
Marker name: collagen, type I, alpha 1
Chromosome: 11
Strain of origin: C57BL/6
Expressed genes: Emv13,endogenous ecotropic MuLV 13,mouse, laboratory
Molecular note: Experimental integration of a proviral genome occurred in the first intron 19 base pairs downstream of the intron/exon boundary, and leads to complete transcriptional block in most cell types, except for odontoblasts and some osteoblasts.
General note: A yeast artificial chromosome (YAC) 150 kb long that spans the Col1a1 gene has been introduced into fibroblasts carrying the Col1a1^Mov13 mutation. The YAC complements the mutant gene, conferring normal collagen RNA expression (J:722). It can also be introduced transgenically into the germ line (J:4495).