B6;C3Sn-Pde6b⁺ eyl5/Boc

Stock number: 002133
Research areas: Developmental Biology Research, Mouse/Human Gene Homologs, Sensorineural Research

Description

Eyeless 5 homozygotes have a high rate of anophthalmia and those with intact globes have microphthalmia and white corneal opacity.

Detailed description

Eyeless 5 homozygotes have a high rate of anophthalmia and those with intact globes have microphthalmia and white corneal opacity. The genetic lesion underlying this spontaneous mutation has not yet been identified. This strain lacks the Pde6b^rd1 allele and carries the Y Chromosome from C3H/HeSn.

Development

The eyeless 5 mutation arose spontaneously in C3H/HeSn-ocd/J and a mutant male was crossed to a C57BL/6J female and their offspring were maintained by sibling intercrossing, selecting for Pde6b⁺ from C57BL/6J and eyl5 and breeding both to homozygosity. At generation N1F26 a breeding depression forced another outcross of a male homozygote to a female C57BL/6J followed again by sibling intercrossing to generate mice homozygous for both eyl5 and Pde6b⁺. Because of the pedigree, this strain happens to carry the Y Chromosome from C3H/HeSn. Sperm was cryopreserved from males homozygous for both eyl5 and Pde6b⁺.

Allele

Symbol: Pde6b⁺
Name: wild type
MGI accession ID: MGI:2430003
Generation method: Not Applicable
Marker symbol: Pde6b
Marker name: phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Marker synonyms: CSNB3; CSNBAD2; GMP-PDEbeta; nmf137; PDEB; r; rd; rd1; rd10; RP40
Chromosome: 5
Strain of origin: Not Applicable
Expressed genes: Pde6b,phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide,mouse, laboratory
Site of expression: Retina.

Allele

Symbol: eyl5
Name: eyeless 5
MGI accession ID: MGI:6359900
Generation method: Spontaneous
Attributes: Not Specified
Marker symbol: eyl5
Marker name: eyeless 5
Chromosome: 12
Strain of origin: C3H/HeSn-ocd/J
Molecular note: This spontaneous heritable phenotype was mapped to Chromosome 12 between D12Mit60 and D12Mit54.