Mice homozygous for the Tcrbtm1Mom targeted mutation are deficient in alpha beta T-cell receptor. Alpha beta thymocyte differentiation is blocked at an earlier stage than the Tcratm1Mom strain. Mice may develop inflammatory bowel disease beginning at ~4-6 months of age.
Peter Mombaerts, Max Planck Research Unit for NeurogeneticsRead More +
Mice homozygous for the Tcrbtm1Mom targeted mutation are viable and fertile. Mice are deficient in alpha beta T-cell receptor. The total number of cells in the thymus is ~8% that of wildtype; CD4+CD8+ cells ~6% of wildtype. The proportion of CD4-CD8- (IL2 receptor positive) cells increases to about 50% of the total cell number. Alpha beta thymocyte differentiation is blocked at an earlier stage than the Tcratm1Mom strain. There is normal differentiation of gamma delta thymocytes. Mice may develop inflammatory bowel disease beginning at ~4-6 months of age.
The Tcrbtm1Mom mutant was developed by Dr. Peter Mombaerts in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology. A targeting replacement vector was used which resulted in a 15-kb deletion encompassing the Tcrb locus from J-beta-1.3 to downstream of C-beta-2. The mutant contains the Pgk-neo gene in reverse transcriptional orientation. The 129-derived E14 ES cell line was used.
|Allele Name||targeted mutation 1, Peter Mombaerts|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CBeta-; TCR-Cbeta-; TCRb-; TCRbeta-; beta-|
|Gene Symbol and Name||Tcrb, T cell receptor beta chain|
|Gene Synonym(s)||TCRbeta; Tib; Tib|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||15kb of sequence was replaced with a PGK-neo cassette. The deletion included one of the two D gene segments, ten of the twelve J gene segments, and both constant gene segments.|
|Mutations Made By|| |
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
This Tcrbtm1Mom strain is maintained by mating homozygous siblings. This strain should be housed under conditions similar to Prkdcscid/Prkdcscid mice or any other immunodeficient strain. It has been cryopreserved and is currently only available from the frozen embryo repository.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
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