Mouse Strain Datasheet - 002101
Mouse Strain Datasheet - 002101
Trp53 knockout mutant mice develop tumors at three to six months of age. They are suitable for use in applications related to the study of familial breast cancers such as Li-Fraumeni syndrome as well as research of lung, brain and bone tumors, lymphoma and leukemia, and other rare cancers.
IMR Colony, The Jackson Laboratory
| Genetic Background | Generation |
|---|---|
|
000664 C57BL/6J |
N5F6pF1+N1F13
|
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Targeted (Null/Knockout) | Trp53 | transformation related protein 53 |
Starting at:
| $231.00 Domestic price for female 4-week |
| 462.00 Domestic price for breeder pair |
A neomycin cassette replaces exons 2-6 (including the start codon) of the transformation related protein 53 gene (Trp53). Mice homozygous for the mutation show no visible phenotype but most develop tumors (principally lymphomas and sarcomas) at three to six months of age. Heterozygous mice develop tumors at about 10 months of age. These mice model some of the features of human Li-Fraumeni syndrome, a form of familial breast cancer with mutations in TRP53. Homozygous mice may produce a litter before succumbing to tumors.
For the C57BL/6-J congenic Trp53tm1Tyj mouse line (Stock No. 002101), it is the experience of The Jackson Laboratory that homozygous males have expected Mendelian survival rates at wean age, but homozygous females have significantly reduced survival by wean age. Specifically, breeding heterozygous females to heterozygous males results in only ~3.5% homozygous females by wean age (expected Mendelian 12.5%). Additionally, breeding heterozygous females to homozygous males results in only ~6.8% homozygous females by wean age (expected Mendelian 25%).
The Trp53tm1Tyj mutant strain was developed in the laboratory of Dr. Tyler Jacks at the Center for Cancer Research at the Massachusetts Institute of Technology. The 129-derived D3 ES cell line was used. The C57BL/6J strain was produced by backcrossing the Trp53tm1Tyj mutation at least five times to C57BL/6J inbred mice.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 8, was segregating with 129.
| Allele Name | targeted mutation 1, Tyler Jacks |
|---|---|
| Allele Type | Targeted (Null/Knockout) |
| Allele Synonym(s) | targeted mutation 1, Tyler Jacks; Trp53tm1Tyj |
| Gene Symbol and Name | Trp53, transformation related protein 53 |
| Gene Synonym(s) | BCC7; LFS1; p44; P53; TRP53; p53; BMFS5 |
| Site of Expression | Normal Trp53 expression is widespread. |
| Strain of Origin | 129S2/SvPas |
| Chromosome | 11 |
| General Note | This mutant allele was produced by a targeted neo insertion into the Trp53 locus. Homozygotes show no visible phenotype but develop tumors at 3-6 months of age. Heterozygotes develop tumors at 10 months of age. These mice model some of the features of human Li-Fraumeni syndrome (OMIM 151623), a form of familial breast cancer with mutations in TRP53 (J:16022)(J:16023) A specific human mutation found in hepatocellular carcinomas caused by hepatitis B infection or by aflatoxin exposure has been created in a mouse model, resulting in a similar gene product (J:27363). |
| Molecular Note | A neomycin cassette replaced 40% of the coding sequences beginning with exon 2 (upstream of the translation start site) and extending into exon 6. |
| Mutations Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology |
This Trp53tm1Tyj strain is maintained by mating heterozygous mice together or by mating heterozygous females with homozygous males.
For the C57BL/6-J congenic Trp53tm1Tyj mouse line (Stock No. 002101), it is the experience of The Jackson Laboratory that homozygous males have expected Mendelian survival rates at wean age, but homozygous females have significantly reduced survival by wean age. Specifically, breeding heterozygous females to heterozygous males results in only ~3.5% homozygous females by wean age (expected Mendelian 12.5%). Additionally, breeding heterozygous females to homozygous males results in only ~6.8% homozygous females by wean age (expected Mendelian 25%).
When using the p53 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002101 in your Materials and Methods section.
| Service | Genotype | Price |
|---|---|---|
| Heterozygous or Wildtype for |
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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