129T2/SvEmsJ

Stock number: 002065
Strain synonyms: 129T2
Research areas: Cancer Research, Dermatology Research, Developmental Biology Research, Mouse/Human Gene Homologs, Reproductive Biology Research, Research Tools

Description

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This is a substrain of the 129 family that does not carry the teratoma gene (Dnd1^Ter).

Detailed description

Historically, the 129 inbred mice are known for the high incidence of spontaneous testicular teratomas, though the incidence differs between substrains. (1-3% in 129 parental substrains; 30% in teratoma substrains.) More recently 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. There is major genetic variation within the 129 "family", which has led to an update of the nomenclature and a division of the substrains into three major groups: parental substrains (129P), steel substrains (129S) and "teratoma" substrains (129T).

The teratoma family of 129 sublines was derived from an outcross of the parental 129/Sv to a hybrid (WC x C57BL/6) to introduce the dominant spotting mutation (Kit^W). After 8 generations of backcrossing to 129/Sv a higher incidence of testicular teratomas was observed. The subline became the 129T1/Sv-Oca2⁺ Tyr^c-ch Dnd1^Ter/J substrain (Stock No. 000091). In 1992 E.M. Simpson selected against the ter allele to establish the present substrain which was transferred to the Jackson Laboratory in 1993.

Investigators using 129 substrains for targeted mutagenesis should be careful in the selection of the appropriate 129 substrain to match the embryonic stem cell line. For a complete history of the numerous 129 substrains, see Simpson, et al., 1997.

Development

Derived from strain 129T1/Sv-Oca2⁺ Tyr^c-ch Dnd1^Ter/J (Stock No. 000091) and selected for the wild type of the Dnd1^Ter by E.M. Simpson in 1992.

Allele

Symbol: Disc1^del
Name: deletion
MGI accession ID: MGI:3623217
Generation method: Spontaneous
Synonyms: Disc1^129S6; Disc1^delta6
Marker symbol: Disc1
Marker name: disrupted in schizophrenia 1
Marker synonyms: C1orf136; SCZD9
Chromosome: 8
Strain of origin: various
Molecular note: A 25 bp deletion in exon 6 causes a frame shift in the reading frame, resulting in 13 novel amino acids and a premature stop codon in exon 7.
General note: This deletion appears in multiple strains of the 129 superfamily, 101/RI, BTBR T⁺ tf/J, LP/J, FVB/NJ, SJL/J, SWR/J and DDY/JclSidSeyFrkJ (J:111837, J:195189).

Allele

Symbol: A^w
Name: white-bellied agouti
MGI accession ID: MGI:1855936
Generation method: Spontaneous
Synonyms: A^L light-bellied agouti
Marker symbol: a
Marker name: nonagouti
Marker synonyms: agouti; agouti signal protein; AGSW; AGTI; AGTIL; As; ASP; SHEP9
Chromosome: 2
Strain of origin: Not Specified
Molecular note: Molecular cloning demonstrated that the location of an exon, 1A, responsible for the ventral-specific light (white) belly is within a 3.1-kb element that is duplicated in the opposite orientation 15-kb upstream producing an interrupted palindrome approximately 22 kb in length.

Allele

Symbol: Tyr^c-ch
Name: chinchilla
MGI accession ID: MGI:1855977
Generation method: Spontaneous
Synonyms: c^ch; c^r
Marker symbol: Tyr
Marker name: tyrosinase
Marker synonyms: ATN; CMM8; Oca1; OCA1A; OCAIA; SHEP3; skc35
Chromosome: 7
Strain of origin: fancier's stock
Molecular note: The mutation in the chinchilla allele was found to be a G-to-A point mutation that results in an amino acid change at position 482 or 464 from alanine to threonine (p.A482T for pre-protein, p.A464T for mature protein).