Historically, the 129 inbred mice are known for the high incidence of spontaneous testicular teratomas, though the incidence differs between substrains. (1-3% in 129 parental substrains; 30% in teratoma substrains.) More recently 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. There is major genetic variation within the 129 "family", which has led to an update of the nomenclature and a division of the substrains into three major groups: parental substrains (129P), steel substrains (129S) and "teratoma" substrains (129T).
The teratoma family of 129 sublines was derived from an outcross of the parental 129/Sv to a hybrid (WC x C57BL/6) to introduce the dominant spotting mutation (KitW). After 8 generations of backcrossing to 129/Sv a higher incidence of testicular teratomas was observed. The subline became the 129T1/Sv-Oca2+ Tyrc-ch Dnd1Ter/J substrain (Stock No. 000091). In 1992 E.M. Simpson selected against the ter allele to establish the present substrain which was transferred to the Jackson Laboratory in 1993.
Investigators using 129 substrains for targeted mutagenesis should be careful in the selection of the appropriate 129 substrain to match the embryonic stem cell line. For a complete history of the numerous 129 substrains, see Simpson, et al., 1997.
Derived from strain 129T1/Sv-Oca2+ Tyrc-ch Dnd1Ter/J (Stock No. 000091) and selected for the wild type of the Dnd1Ter by E.M. Simpson in 1992.
|Allele Name||white-bellied agouti|
|Allele Synonym(s)||AL light-bellied agouti|
|Gene Symbol and Name||a, nonagouti|
|Strain of Origin||Not Specified|
|Molecular Note||Molecular cloning demonstrated that the location of an exon, 1A, responsible for the ventral-specific light (white) belly is within a 3.1-kb element that is duplicated in the opposite orientation 15-kb upstream producing an interrupted palindrome approximately 22 kb in length.|
|Allele Synonym(s)||Disc1129S6; Disc1delta6|
|Gene Symbol and Name||Disc1, disrupted in schizophrenia 1|
|Strain of Origin||various|
|General Note||This deletion appears in multiple strains of the 129 superfamily, 101/RI, BTBR T+ tf/J, LP/J, FVB/NJ, SJL/J, SWR/J and DDY/JclSidSeyFrkJ (J:111837, J:195189).|
|Molecular Note||A 25 bp deletion in exon 6 causes a frame shift in the reading frame, resulting in 13 novel amino acids and a premature stop codon in exon 7.|
|Allele Synonym(s)||cch; cr|
|Gene Symbol and Name||Tyr, tyrosinase|
|Strain of Origin||fancier's stock|
|Molecular Note||The mutation in the chinchilla allele was found to be a G-to-A point mutation that results in an amino acid change at position 482 or 464 from alanine to threonine (p.A482T for pre-protein, p.A464T for mature protein).|
When using the 129T2/SvEmsJ mouse strain in a publication, please include JAX stock #002065 in your Materials and Methods section.
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