In this congenic strain, the H2 complex derived from DBA/1 (H2q) was introgressed onto the C57BL/10SnSg background. Mice congenic for H2 haplotypes are widely used in immunologic research and often vary in immune response and pathogen susceptibility from the recipient strain.
The Jackson Laboratory
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | H2 | histocompatibility-2, MHC |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Tyk2 | tyrosine kinase 2 |
This strain is homozygous for a naturally occurring missense mutation in Tyk2 resulting in a loss of protein function. The mutation is associated with lymphocyte hyporesponsiveness to interleukin 12. Disruption of the IL12 signaling pathway results in an increased susceptibility to intracellular pathogens such as Toxoplasma gondii and a resistance to autoimmune disease such as collagen induced arthritis.
In this congenic strain, the H2 complex derived from DBA/1 (H2q) was introgressed onto the C57BL/10SnSg background. H2q occurs in the AU, BUB, FVB and SWR inbred strains. The H2q haplotype is q at the class I (K,D) loci and the class II loci (A, E) and b at T18. T18b is a natural deletion of the T18 gene. Mice congenic for H2 haplotypes are widely used in immunologic research and often vary in immune response and pathogen susceptibility from the recipient strain.
The DBA/1 H2 complex (H2q) was introgressed onto the C57BL/10SnSg background for 11 generations with selection for H2q at each generation.
Allele Name | q variant |
---|---|
Allele Type | Not Applicable |
Allele Synonym(s) | |
Gene Symbol and Name | H2, histocompatibility-2, MHC |
Gene Synonym(s) | |
Strain of Origin | Not Applicable |
Chromosome | 17 |
General Note | The q variant has been observed in the following strains: DBA/1, DBA/1J, BUB/BnJ, STOLI/Lw, SWR/J Genbank ID for this allele: M14827 |
Allele Name | E775K |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | Tyk2A |
Gene Symbol and Name | Tyk2, tyrosine kinase 2 |
Gene Synonym(s) | |
Strain of Origin | B10.D1-H2q/SgJ |
Chromosome | 9 |
Molecular Note | This allele corresponds to a substitution of G to A at coding nucleotide 2335 (transcript NM_018793), resulting in an amino acid substitution that replaces glutamic acid with lysine at position 779 (p.E779K). The missense mutation occurs at highly conserved APE motif of the Jak homology 2 (JH2) domain. Although mRNA transcript is detectable in spleen, anti-Tyk2 specific antibody cannot detect protein either in the activated or inactivated form. |
rs50413633
The Jackson Laboratory has not developed a genotyping protocol for H2 haplotypes. The following reference may helpful: Saha BK. J Immunol Meth 1996 Jul 17;194(1):77-83. (PMID: 8690943). Alternatively, haplotype-specific antibodies can be used with FACS.
When using the B10.Q mouse strain in a publication, please cite the originating article(s) and include JAX stock #002024 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous, 1 pair minimum |
Frozen Mouse Embryo | B10.D1-H2<q>/SgJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B10.D1-H2<q>/SgJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B10.D1-H2<q>/SgJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B10.D1-H2<q>/SgJ Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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