B10.D1-H2^q/SgJ

Stock number: 002024
Product name: B10.Q
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

In this congenic strain, the H2 complex derived from DBA/1 (H2^q) was introgressed onto the C57BL/10SnSg background. Mice congenic for H2 haplotypes are widely used in immunologic research and often vary in immune response and pathogen susceptibility from the recipient strain.

Detailed description

In this congenic strain, the H2 complex derived from DBA/1 (H2^q) was introgressed onto the C57BL/10SnSg background. H2^q occurs in the AU, BUB, FVB and SWR inbred strains. The H2^q haplotype is q at the class I (K,D) loci and the class II loci (A, E) and b at T18. T18^b is a natural deletion of the T18 gene. Mice congenic for H2 haplotypes are widely used in immunologic research and often vary in immune response and pathogen susceptibility from the recipient strain.

Development

The DBA/1 H2 complex (H2^q) was introgressed onto the C57BL/10SnSg background for 11 generations with selection for H2^q at each generation.

Allele

Symbol: H2^q
Name: q variant
MGI accession ID: MGI:3579324
Generation method: Not Applicable
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Chromosome: 17
Strain of origin: Not Applicable
General note: The q variant has been observed in the following strains: DBA/1, DBA/1J, BUB/BnJ, STOLI/Lw, SWR/J

Genbank ID for this allele: M14827

Allele

Symbol: Tyk2^E775K
Name: E775K
MGI accession ID: MGI:3706656
Generation method: Spontaneous
Marker symbol: Tyk2
Marker name: tyrosine kinase 2
Chromosome: 9
Strain of origin: B10.D1-H2^q/SgJ
Molecular note: This allele corresponds to a substitution of G to A at coding nucleotide 2335 (transcript NM_018793), resulting in an amino acid substitution that replaces glutamic acid with lysine at position 779 (p.E779K). The missense mutation occurs at highly conserved APE motif of the Jak homology 2 (JH2) domain. Although mRNA transcript is detectable in spleen, anti-Tyk2 specific antibody cannot detect protein either in the activated or inactivated form.