In this congenic strain, the H2 complex derived from DBA/1 (H2q) was introgressed onto the C57BL/10SnSg background. Mice congenic for H2 haplotypes are widely used in immunologic research and often vary in immune response and pathogen susceptibility from the recipient strain.
The Jackson Laboratory
This strain is homozygous for a naturally occurring missense mutation in Tyk2 resulting in a loss of protein function. The mutation is associated with lymphocyte hyporesponsiveness to interleukin 12. Disruption of the IL12 signaling pathway results in an increased susceptibility to intracellular pathogens such as Toxoplasma gondii and a resistance to autoimmune disease such as collagen induced arthritis.
In this congenic strain, the H2 complex derived from DBA/1 (H2q) was introgressed onto the C57BL/10SnSg background. H2q occurs in the AU, BUB, FVB and SWR inbred strains. The H2q haplotype is q at the class I (K,D) loci and the class II loci (A, E) and b at T18. T18b is a natural deletion of the T18 gene. Mice congenic for H2 haplotypes are widely used in immunologic research and often vary in immune response and pathogen susceptibility from the recipient strain.
The DBA/1 H2 complex (H2q) was introgressed onto the C57BL/10SnSg background for 11 generations with selection for H2q at each generation.
|Allele Name||q variant|
|Allele Type||Not Applicable|
|Gene Symbol and Name||H2, histocompatibility-2, MHC|
|Strain of Origin||Not Applicable|
|General Note||The q variant has been observed in the following strains: DBA/1, DBA/1J, BUB/BnJ, STOLI/Lw, SWR/J |
Genbank ID for this allele: M14827
|Gene Symbol and Name||Tyk2, tyrosine kinase 2|
|Strain of Origin||B10.D1-H2q/SgJ|
|Molecular Note||This allele corresponds to a substitution of G to A at coding nucleotide 2335 (transcript NM_018793), resulting in an amino acid substitution that replaces glutamic acid with lysine at position 779 (p.E779K). The missense mutation occurs at highly conserved APE motif of the Jak homology 2 (JH2) domain. Although mRNA transcript is detectable in spleen, anti-Tyk2 specific antibody cannot detect protein either in the activated or inactivated form.|
The Jackson Laboratory has not developed a genotyping protocol for H2 haplotypes. The following reference may helpful: Saha BK. J Immunol Meth 1996 Jul 17;194(1):77-83. (PMID: 8690943). Alternatively, haplotype-specific antibodies can be used with FACS.
When using the B10.Q mouse strain in a publication, please cite the originating article(s) and include JAX stock #002024 in your Materials and Methods section.