Overview

Also Known As:dense incisors

These mice carry a spontaneous mutation at the 4930453N24Rik locus characterized by arrested eruption of incisors.

Genetic overview

Genetic Background	Generation

a

Allele Type	Gene Symbol	Gene Name
Spontaneous	a	nonagouti

Allele Type	Gene Symbol	Gene Name
Spontaneous	4930453N24Rik	RIKEN cDNA 4930453N24 gene

Research Applications

Dermatology Research
Developmental Biology Research
Internal/Organ Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for the dense incisors mutation have normal initial eruption of incisors through the gingiva, but further eruption is arrested. As a result continued dentin formation gradually occludes the pulp chambers resulting in dense teeth and an anterior open bite. The molars erupt normally. Homozygotes also have a smaller than normal body size, smaller than normal ear pinna, and pigment dilution that is more pronounced in the ventral fur than the dorsal fur and causes white feet and a white tail tip. The white feet and tail tip are not distinguishing features of this mutation, rather the lack of incisor eruption at 10 days of age, small ear pinnae, and dilution of the ventral fur pigment at 20 days of age are distinguishing features on this background. Most homozygotes die before 5 months of age, and this is not altered by the addition of powdered food to the bottom of the cage. Homozygotes are not successful breeders and although female homozygotes have become pregnant they have failed to deliver. When outcrossed to CAST/EiJ and intercrossed the homozygotes have a more variable phenotype, with half displaying lower incisors at 10 days of age despite having the proportionate dwarf and diluted ventral coat color phenotypes. The diluted ventral coat color is more severe in the segregating CAST/EiJ background.

Development

The dense incisors mutation (din) arose spontaneously in strain C3H/HeJ at F181 in 1984 at the Jackson Laboratory. It was sibling mated and ovaries from a homozygous female were transplanted into a B6C3Fe-a/a F1 host and mated to a C57BL/6J male. The offspring were sibling mated and an ovary transplant was made in the next generation and crossed to the B6C3Fe-a/a hybrid. The mutation was maintained on this hybrid background using the cross intercross system with ovary transplantation. It was cryopreserved in 1992 by mating din/+ males to din/+ females at N19.

Control Suggestions

Untyped from the colony

Additional Information

Considerations for Choosing Controls

Genetics

a

Allele Symbol: a

Allele Name	nonagouti
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	a, nonagouti
Gene Synonym(s)
Strain of Origin	old mutant of the mouse fancy
Chromosome	2
General Note	Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039
Molecular Note	Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for a^t-2Gso and A^w-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.

4930453N24Rik^din

Allele Symbol: 4930453N24Rik^din

Allele Name	dense incisors
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	4930453N24Rik, RIKEN cDNA 4930453N24 gene
Gene Synonym(s)
Strain of Origin	C3H/HeJ
Chromosome	16
Molecular Note	A T-to-A point mutation at postition 729 of the cDNA (NM_026273) results in premature truncation at position 243 of the encoded protein (p.C243*).

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: 4930453N24Rik^din related

Dermatology Research
- Color and White Spotting Defects
Developmental Biology Research
- Skeletal Defects
- Growth Defects
  - Growth Defects (homozygous)
- Craniofacial and Palate Defects
Internal/Organ Research
- Skeleton
  - teeth

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: 4930453N24Rik^din/4930453N24Rik^din
B6C3Fe a/a-4930453N24Rik/J

craniofacial phenotype

abnormal dentin development
- continued dentin formation after arrested tooth eruption occludes pulp chamber creating a dense incisor

abnormal dentin morphology
- incisors show increased dentin thickness in older mice

abnormal incisor morphology
- continued dentin formation after arrested incisor eruption occludes pulp chamber creating a dense incisor

failure of tooth eruption
- incisors begin to erupt but cease erupting after initial penetration of the oral epithelium
- Normal - molar euption is unaffected

malocclusion
- failure of continued incisor eruption produces an open bite anteriorly, rendering these teeth useless for chewing and gnawing

small ears

hearing/vestibular/ear phenotype

small ears

integument phenotype

abnormal coat/hair pigmentation
- the ventral coat is lighter resembling a^t or A^w;white feet and tail tip observed; yellow hair absent from ears

irregular coat pigmentation
- abnormal dorsoventral pigment pattern resembles a^t and A^w

mortality/aging

premature death
- most die before 5 months of age

pigmentation phenotype

abnormal coat/hair pigmentation
- the ventral coat is lighter resembling a^t or A^w;white feet and tail tip observed; yellow hair absent from ears

irregular coat pigmentation
- abnormal dorsoventral pigment pattern resembles a^t and A^w

skeleton phenotype

abnormal dentin development
- continued dentin formation after arrested tooth eruption occludes pulp chamber creating a dense incisor

abnormal dentin morphology
- incisors show increased dentin thickness in older mice

abnormal incisor morphology
- continued dentin formation after arrested incisor eruption occludes pulp chamber creating a dense incisor

failure of tooth eruption
- incisors begin to erupt but cease erupting after initial penetration of the oral epithelium
- Normal - molar euption is unaffected

malocclusion
- failure of continued incisor eruption produces an open bite anteriorly, rendering these teeth useless for chewing and gnawing

growth/size/body region phenotype

abnormal dentin development
- continued dentin formation after arrested tooth eruption occludes pulp chamber creating a dense incisor

abnormal dentin morphology
- incisors show increased dentin thickness in older mice

abnormal incisor morphology
- continued dentin formation after arrested incisor eruption occludes pulp chamber creating a dense incisor

failure of tooth eruption
- incisors begin to erupt but cease erupting after initial penetration of the oral epithelium
- Normal - molar euption is unaffected

malocclusion
- failure of continued incisor eruption produces an open bite anteriorly, rendering these teeth useless for chewing and gnawing

postnatal growth retardation
- mean body weight increasingly differs from normal littermates after the first postnatal week and is not caused by abnormal dentition

small ears

References

Additional - a related

Additional - 4930453N24Rik^din related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Appearance
- black-gray underfur and white ear
  Related Genotype: a/a din/din
  
  black
  Related Genotype: a/a din/+ or a/a +/?
Citation
When using the dense incisors mouse strain in a publication, please cite the originating article(s) and include JAX stock #002018 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous, Heterozygous or wildtype for din, 1 pair minimum

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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